| SWI4 deletion | Deletion of SWI4 shortens replicative lifespan by approximately 90% [11805047]. SSD1-V partially suppresses the short lifespan of a swi4 mutant.
Mutation of swi4 results in slow growth and temperature sensitivity, both of which are suppressed by SSD1-V [11805047]. | Yeast | -90 | — | — |
| NNT1 deletion | Deletion of NNT1 decreases mean and maximum lifespan by 9 and 19%. 0.5% glucose DR extends the mean and maximum lifespan of NNT1 deletion mutants by 35 and 40%. NNT1 deletion decreases rDNA silencing [12736687]. | Yeast | -9 | — | -19 |
| NCA3 deletion | Disruption in NCA3 shortens mean (87% of normal), nut not maximum replicative lifespan without causing any other gross changes in cell cycle parameters of growth characteristics [8810036].
In combination with an NCA2 disruption, NCA3 disruption causes a cryosensitive phenotype on non-fermentable carbon sources due to a defect in the F1-F0 ATP synthetase due to misbalancing of alternate spliceforms of mitochondrial mRNA encoding subunits 6 and 8 of the synthase [7586026]. | Yeast | -87 | — | — |
| VMA2 deletion | VMA2 deletion mutants have a reduced ΔΨ and mitochondrial morphology similar to aged cells. The restoration of the vacuolar acidity in daughter cells requires V-ATPase activity as it is eliminated in VMA2 deletion mutant cells [23172144]. VMA2 deletion mutation decreases the mean replicative lifespan by 80% in the alpha strain [18340043]. Deletion of VMA2 decreases mean, median and maximum replicative lifespan by 84%, 84% and 70%, respectively. DR (0.5% glucose restriction) does not extend the replicative lifespan of VMA2 and shortens it even more [23172144]. | Yeast | -80 to -83.9 | -84.1 | -70.0 |
| CCR4 deletion | Deletion of CCR4 increases mean chronological lifespan by 20 - 41% (20, 33, 41) in diploid cells [21447998]. In W303R CCR4 deletion shortens replicative lifespan by approximately 80% and results in temperature sensitivity that is suppressed by SSD1-V. SSD1-V partially suppresses the short-lifespan of ccr4 mutant. CCR4 mutation is synthetically lethal in combination with deletion of MPT5 in the absence of SSD1-V [11805047]. | Yeast | -80 to +20 | — | — |
| SIP1 deletion | Deletion of SIP1 decreases replicative lifespan by 80%, without accompying aging biomarkers in S288C strain [10921902]. | Yeast | -80 | — | — |
| RAD52 deletion | Deletion in RAD52 causes a 75% reduction in mean replicative lifespan in PSY316 strain [10207108]. Similiar reduction of lifespan occurs in strains W3031-A and W303R [M. Baeberlein, M. McVey, and L. Guarente, unpublished].
RAD51 is required for formation of extrachromosomal rDNA circles [10207108], but not for replication fork pasuing nor DNA breakage with the rDNA [10693764]. | Yeast | -75 | — | — |
| TPS1 deletion | Deletion of TPS1 decreases intracellular trehalose concentration and shortens the mean chronological lifespan by 74% (at 30 degree Celsus in BY4742) [22783207]. | Yeast | -74 | — | — |
| SOD2 deletion | SOD2 deletion decreases replicative lifespan by 72% [17460215]. SOD2 deletion decreases chronological lifespan [21076178]. Deletion of SOD2 decreases chronological lifespan in wild-type and abolishes chronological lifespan extension in sch9Delta mutants as well as decreases chronological lifespan in cyr1:mTn mutants [12586694].
SOD2 deletion mutants are hypersensitive to oxygen and grow poorly in ethanol [10222047]. | Yeast | -72 | — | — |
| ATP1 deletion | Deletion of ATP1 increases chronological lifespan by up to 50% [17492370], but decreases replicative lifespan by 70% in the alpha strain [18340043]. | Yeast | -70 to +50 | — | — |
| Top3b knockout | Homozygous disruption of Top3b results in a normal development but a shorter lifespan (by approximately 70%) accompanied by lesions in multiple organs in C57BL/6 [11331780]. | Mouse | -70 | — | — |
| INM2 deletion | Deletion of INM2 decreases replicative lifespan by 70% in the alpha strain [19030232]. | Yeast | -70 | — | — |
| RAD50 deletion | RAD50 mutations result in a 70% reduced replicative lifespan [10207108]. | Yeast | -70 | — | — |
| TPS2 deletion | Deletion of TPS2 has no effect on replicative lifespan and does not prevent lifespan extension by high osmolarity [12391171]. Deletion of TPS2 decreases intracellular trehalose concentration and shortens mean chronological lifespan by 61% (at 30 degree Celsus in BY4742) [22783207].
TPS2 is required for trehalose biosynthesis in response to osmotic stress [8444170]. | Yeast | -61 | — | — |
| SIP2 deletion | Deletion of the N-myristoylprotein SIP2 results in reduced resistance to nutrient deprivation and a 60% shorter lifespan accompanied by signs of accelerated aging such as loss of silencing from telomeres and mating loci, redistribution of Sir3 to the nucleolus, progressive sterility, nucleolar fragmentation and enlargement, and accumulation of extrachromosomal rDNA [10921902].
SIP2 deletion increases replicative lifespan by 20% in the alpha strain [19030232].
SIP1 null mutation causes increase in the intracellular ATP and NAD+ levels in both young cells (generation 0-1) and older cells (generation 4), but the increase is greater in older cells [10921902]. | — | -60 to +20 | — | — |
| RAD27 deletion | Deletion of RAD27 results in signs of premature aging and approximately 60% reduction in mean replicative lifespan [12024027]. | Yeast | -60 | — | — |
| YGR071C deletion | Deletion of YGR071C decreases replicative lifespan by 60% in the alpha strain [19030232]. | Yeast | -60 | — | — |
| CLA4 deletion | Deletion of CLA4 decreases replicative lifespan by 60% in the alpha strain [18340043; 19030232]. | Yeast | -60 | — | — |
| VAM3 deletion | Deletion of VAM3 decreases mean replicative lifespan by 56% both on AL [19030232] and on moderate DR [18690010] (in BY4742/alpha strain). | Yeast | -56 | — | — |
| SRS2 deletion | Deletion of SRS2 shortens mean replicative lifespan by 50% [11290710]. Overexpression of SGS1 increases maximum, but not mean lifespan of SRS2 mutants [11861900]. Deletion of SRS2 is synthetical lethal in combination with deletion of SGS1 [11290710]. | Yeast | -50 | — | — |
| LAG2 deletion | Deletion of LAG2 in haploid SP1 strain does not affect growth, but results in a 50% decrease in the mean and maximum replicative lifespan [8760941]. | Yeast | -50 | — | -50 |
| RRG1 deletion | Deletion of RRG1 decreases replicative lifespan by 50% in the alpha strain [19030232]. | Yeast | -50 | — | — |
| GMC2 deletion | Deletion of GMC2 decreases replicative lifespan by 50% in the alpha strain [19030232]. | Yeast | -50 | — | — |
| YNR066C deletion | Deletion of YNR066C decrease replicative lifespan by 50% in the alpha strain [19030232]. | Yeast | -50 | — | — |
| NPT1 deletion | NPT1 deletion decreases replicative lifespan by 50% [17482543] as well as chronological lifespan [17110466]. Deletion of NPT1 shortens the lifespan in W303R. Replicative lifespan extension of cdc25-10 mutation (assumed to act as a genetic DR-mimetic) is cancelled out by NPT1 deletion [11000115].
NPT1 mutation results in loss of telomere and rDNA silencing [10841563], an effect that is likely caused by a loss of SIR2 activty due to decreased NAD levels. Mutation of NPT1 is synthetical lethal with mutation of QPT1 [11000115]. | Yeast | -50 | — | — |
GenAge
GenDR
