| YGL235W | — | YGL235W increases replicative lifespan by 20% in the alpha strain [19030232]. | Budding yeast |
| TRM9 | TRna Methyltransferase 9 | TRM9 deletion almost triples mean chronological lifespan under starvation/extreme DR, increases heat resistance, but reduces resistance to acetic acid. Similar effect were present in the BY746 background in SDC medium [20657825]. | Budding yeast |
| IPT1 | InositolPhosphoTransferase 1 | Transposon-mediated mutation of IPT1 increases oxidative stress resistance and chronological lifespan by 40% [16527275]. IPT1 deletion decreases replicative lifespan by 30% in the alpha strain [19030232]. | Budding yeast |
| SCH9 | — | Transposon-mediated mutagenesis of SCH9, which encodes for a serine threonine kinase homologous to Akt/PKB, increases resistance to oxidants and thermal stress as well as extends chronological lifespan by 30%. SCH9 deletion increases chronological lifespan by up to threefold. Stress-resistance transcription factors Msn2/Msn4 and protein kinase Rim15 are required for this life-extension. Deletion of the mitochondrial antioxidant enzyme superoxide dismutase gene SOD2 prevents the increased chronological lifespan caused by SCH9 deletion [11292860]. Mutations that decrease the activity of the Ras/Cyr1/PKA pathway also extend longevity and increase stress resistance by activating transcription factors Msn2/Msn4 and Sod2 [12855292]. SCH9 deletion mutants exhibit more than 3-fold extension of chronological lifespan. By day 9 of medium depletion all the wild-type cells were dead while 50% sch9 mutants survived [17710147]. Deletion of SCH9 also increases resistance to heat shock and oxidative stress [11292860], and increases replicative lifespan by 18% (in DBY746) [12586694]. SCH9 deletion increases the replicative lifespan by 40% in the alpha strain [18340043] and increases mean chronological lifespan by 97 - 246% (97, 133, 154, 226, 246) in diploid cells [21447998]. Mutation or deletion of SCH9 increases resistance to oxidants and extends chronological lifespan [11292860; 16286010]. The extended lifespan of SCH9 deletion mutants is not further extended by low glucose DR and is independent of Sir2 [16293764]. Deletion of RIM15 or GIS1 reverses chronological lifespan extension associated with sch9Delta. Water restriction further increases chronological lifespan of sch9Delta [18225956]. Deletion of SCH9 results in a longer chronological lifespan [21076178]. | Budding yeast |
| TOR1 | Target Of Rapamycin 1 | TOR1 deletion extends mean and maximum replicative lifespan by 21 and 25% [16293764] as well as chronological lifespan [21076178]. This lifespan extension is independent of SIR2 and additive with deletion of FOB1 [16293764]. Deletion of TOR1 fails to increase the replicative lifespan of a sir2 mutant [20947565]. Deletion of TOR1 substantially extends chronological lifespan, increasing median survival almost 3-fold (wild-type 4.5 days, tor1 null 12 days), i.e. by 167%. By 21 days in culture, the vast majority of wild-type cells had died (>99.9%), whereas many tor1 null cells remained viable. Deletion of TOR1 also extends the chronological lifespan of the relatively short-lived BY4742 strain, one of the two haploid genetic backgrounds of the widely used Yeast Knockout Collection available from Open Biosystems. Deletion of TOR1 fails to extend chronological lifespan in Petite strains that are unable to respire [17403371]. TOR1 deletion increases replicative lifespan by 30% in the alpha strain and 20% in a strain [19030232]. TOR1 deletion mutant have and increased mean and maximum replicative lifespan by 21% and 6%, respectively [21931558]. Deletion of TOR1 extends replicative lifespan as well as chronological lifespan [21076178] and glucose restriction fails to further extend the long replicative lifespan of tor1Delta [16293764; 16418483; 18225956]. Water starvation (extreme DR) further extends chronological lifespan of tor1 mutants [18225956]. | Budding yeast |
| CDC6 | Cell Division Cycle | The CDC6-1 conditional allele results in an approximately 20% increase in mean replicative life span. This allele is defective for replicative initiation form the rDNA ARS at 27 degree Celsius, resulting in a reduced rate of extrachromosomal rDNA circle accumulation [9428525]. The cdc6-1 allele results in genomic instability at the permissive temperature [8552037]. | Budding yeast |
| CYR1 | CYclic AMP Requirement 1 | The CDC35-1 allele of the adenylate cyclase CYR1 confers a 75% extension of replicative lifespan at 25 degree Celsius [11000115]. Transposon-mutagenized CYR1 increases resistance to oxidants and extends chronological lifespan by up to 90%. Stress-resistance transcription factors Msn2/Msn4 and protein kinase Rim15 are required for this lifespan extension [11292860]. CYR1 mutation is assumed to act as genetic DR mimetic [11000115]. The CDC35-1 allele of the adenylate cyclase CYR1 confers a 75% extension of replicative lifespan at 25 degree Celsius [11000115]. cyr1-1 mutation extends median chronological lifespan by 28-47% and is non-addative with lifespan extension conferred by overxpression of human MAPK1 [17662940]. | Budding yeast |
| CDC25 | Cell Division Cycle 25 | The CDC25-10 allele extends mean and maximum replicative lifespan by 34% and 18%, respectively, at 30 degree Celsius. cdc25-10 mutants have an extended replicative lifespan under AL. Growth on 0.5% glucose restriction does not further extend replicative lifespan of cdc25-10 mutants. CDC25 null mutant is not viable. CDC25 appears to act in the same genetic pathway as SIR2 and NPT1 and is suggested to be genetic model of DR [11000115]. | Budding yeast |
| TCO89 | Tor Complex One | TCO89 deletion increases chronological lifespan, increases mitochondrial oxygen consumption, but decreases mitochondrial and cellular ROS in early stationary phase [21641548]. Deletion of TCO89 cancels out replicative lifespan extension by moderate DR [18690010]. | Budding yeast |
| SWH1 | — | SWH1 (alias OSH1) deletion mutants have an extended replicative lifespan (p=0.02) and DR does not increase the long lifespan of SWH1 deletion mutants [Xia et al. unpublished]. | Budding yeast |
| SIT4 | Suppressor of Initiation of Transcription 4 | SIT4 deletion slightly increases chronological lifespan and totally abolishes the lifespan shortening due to ISC1 deletion [21707788]. | Budding yeast |
| RTG3 | ReTroGrade regulation 3 | RTG3 deletion mutation causes an increase in mean replicative in lifespan by 55% increase at 2% glucose, suggesting that expression of genes regulated by Rtg1-Rtg3 has a negative effect on longevity in 2% glucose (in YPK9). A null mutant has 123% increases mean lifespan at 0.1% glucose relative to a wild-type strain at 2% glucose, indicating that reduced glucose and an RTG3 mutation have an additive effect on lifespan (in YPK9) [11024000].
RTG3 null mutant cannot grow on acetate medium and requires glutamate or aspartate for growth [9032238]. | Budding yeast |
| RPS6B | — | RPS6B deletion increases mean replicative lifespan by about 30% [16293764]. Deletion of RPS6B, but not of the RPS6A paralog increases replicative median lifespan robustly by 45% [17174052]. | Budding yeast |
| REI1 | Cytoplasmic pre-60S factor | REI1 deletion increases mean replicative lifespan by about 40% [16293764] in the alpha and a strains [19030232]. | Budding yeast |
| RCR2 | Resistance to Congo Red 2 | RCR2 deletion extends mean replicative lifespan by 18% and cancels out the lifespan extending effect of DR [22912585]. | Budding yeast |
| PKH2 | Pkb-activating Kinase Homolog 2 | PKH2 deletion increases replicative lifespan by 20% in the alpha strain and by 15% in the a strain [18340043]. Deletion of PKH2 increases chronological lifespan by 29% [22319457] to 34% [21447998] as well as by 19 - 54% (19, 24, 29, 54) in diploid cells [21447998]. PKH2 mutation extends both replicative and chronological lifespan as well as cancels out DR-induced replicative and chronological lifespan extension [21584246]. Mean and maximum replicative lifespan on AL is extended by 38 and 69%, respectively. | Budding yeast |
| CLN3 | CycLiN 3 | Overexpression shortens chronological lifespan together with age-dependent increases in genome instability and apoptosis. While around 80% of wild-type cells are alive almost non CLN3 overexpressers are alive (under condition that avoids adaptive regrowth) [17710147]. | Budding yeast |
| YDC1 | Yeast DihydroCeramidase 1 | Overexpression of YDC1 decreases chronological lifespan by 40% [19059240] | Budding yeast |
| SSD1 | Suppressor of SIT4 Deletion 1 | Overexpression of SSD1 (addition of a SSD1-V allele) increases replicative lifespan by 50%, independently of SIR2 and SIR2 further extends the lifespan, although SIR2 is necessary for SSD1-V cells to attain maximal lifespan [15126388]. SSD1-V also dramatically increases chronological lifespan with lifespan twice as long as ssd1-d cells [19570907]. Deletion of SSD1 increases replicative lifespan by 50% [Li et al., 2009].
Addition of SSD1-V allele to an ssd1-d strain suppresses the short lifespan of an MPT5 deletion mutant [11805047] and extend wild-type lifespan [Kaeberlein and Guarente, unpublished].
SSD1-V slightly extends the lifespan of swi4 and ccr4 mutant strains and suppresses the temperature sensitive growth phenotype of mpt5, ccr3, swi4, and swi6 single mutants [11805047]. SSD1-V also suppresses the synthetic lethality caused by deletion of MPT5 in combination with a mutation in SWI4, SWI6, or CCR4 [11805047]. SSD1-V suppresses mutations that affect cell wall stability [1545797; 8386319], RNA polymerase III activity [8510644], RNA splicing [10446233], and PKA activity [1848673; 8200529]. | Budding yeast |
| RAS2 | Ras-like protein 2 | Overexpression of RAS2 causes a 43% increase in mean and 18% increase in maximum lifespan as well as postpones the age-related increase in generation time. RAS2 deletion causes a 23% decrease in mean and a 30% decrease in maximum lifespan [8034612]. Deletion of RAS2 leads to a longer chronological lifespan [21076178]. Deletion of the RAS2 gene, which functions upstream of CYR1, doubles the mean chronological lifespan by a mechanism that requires Msn2/4 and Sod2 [12586694]. DR further extends chronological lifespan of ras2Delta [18225956]. | Budding yeast |
| NDE1 | NADH Dehydrogenase, External 1 | Overexpression of NDE1 and NDE2 increases intracellular NAD/NADH ratio by lowering NADH concentration and increases replicative lifespan by 20-25%. This lifespan extension is non-additive 0.5% glucose restriction [14724176]. Deletion of NDE1 extends chronological lifespan [16436509]. | Budding yeast |
| CTT1 | CaTalase T 1 | Overexpression of cytosolic catalase T CTT1 alone slightly shortens stationary phase survival in strain DBY746. Overexpression CTT1 in combination with SOD1 increases stationary phase survival by about 10% [12586694]. Mutational inactivation [20696905] or deletion [21076178] of CTT1 lead to a longer chronological lifespan. | Budding yeast |
| OPT2 | OligoPeptide Transporter 2 | OPT2 deletion increases mean and maximum replicative lifespan by 23 and 9%, respectively, and cancels out the lifespan-extending effect of DR [22912585]. | Budding yeast |
| UTH1 | yoUTH 1 | Mutation in UTH1 increases replicative lifespan by approximately 15% [7859289].
UTH1 mutant has enhanced stress resistance to cold stress [7859289], heat shock [10870102], starvation [7859289], hydrogen peroxide, but is sensitive to paraquat and oxygen [9799359]. | Budding yeast |
| FOB1 | FOrk Blocking less 1 | Mutation in FOB1 extends replicative lifespan by 30-50% [10230397]. FOB1 mutation increases replicative lifespan by 25% in the alpha strain and by 10% in a strain [19030232]. FOB1 mutant exhibit an about 20% mean replicative lifespan increase [15722108]. Deletion of FOB1 causes extension in the short life span of the sir2 mutant by around 50% [10521401]. Mutation of the FOB1 gene slows the generation of rDNA circles and thus extends lifespan by approximately 30% in W303 and 50% in K2307 [10230397]. Even in cells lacking both Sir2 and Fob1, nicotinamide prevents the lifespan extension by DR [16311627]. | Budding yeast |
