| SUN4 | — | Disruption of SUN4 shortens mean (87.5% of normal), but not maximum, replicative lifespan in BKY1-14c [Austriaco, N.R. (1996) âUTH1 and the Genetic Control of Aging in the Yeast, Saccharomyces cerevisiae.â Ph.D. Thesis, Massachusetts Institute of Technology; 8810036]
SUN4 mutation causes failure of daughter cells to completely detach and results in a multi-budded morphology [10683261]. | Budding yeast |
| STE12 | STErile 12 | Deletion of STE12 has no effect on replicative lifespan in strain PSY142 or BKY1-14c [7859289].
STE12 null mutants are sterile [6993497]. | Budding yeast |
| SLT2 | Suppression at Low Temperature 2 | Deletion of SLT2 has no effect on replicative lifespan in W303 strain [12640455].
SLT2 deletion increases rDNA silencing and rDNA recombination and decreases silencing at the telomeres and HM loci [Ray et al., 2003] as well as results in decreased phosphorylation of Sir3 [12640455]. | Budding yeast |
| SIM1 | Start Independent of Mitosis 1 | Disruption of SIM1 shortens mean (87.5%), but not maximum, lifespan without causing any other gross changes in cell cycle parameter or growth characteristics [8810036].
Cells bearing deletions in CLB1-4 are unable to undergo mitosis and normally arrest in G2. SIM1 disruption in clb1-4 mutant backgrounds will allow a second round of DNA synthesis without mitosis [8574583]. sim1delta;uth1delta double mutants exhibit various defects, including binucleated cells, benomyl sensitivity, heat shock sensitivity, inability to store glycogen, sensitivity to starvation and failure of spores to germinate [10612745]. | Budding yeast |
| RTG2 | ReTroGrade regulation 2 | RTG2 is required for replicative lifespan extension associated with the retrograde response, a pathway that signals the functional status of mitochondria to the nucleus to regulate the expression of several genes [11024000]. RTG2 is not required for replicative lifespan extension by DR [11024000].
RTG2 null mutants are not petite [8422683], but display various nutrient auxotrphies and alterations of carbohydrate metabolism [7727418]. | Budding yeast |
| RSR1 | RaS-Related 1 | Deletion of RSR1 (alias BUD1) shortens replicative lifespan [9789734]. | Budding yeast |
| RAD7 | RADiation sensitive 7 | Deletion of RAD7 has no effect on replicative lifespan in PSY316 [10207108].
Mutation in RAD7 results in decrease repair of the non-transcribed strand in rDNA [8604332]. | Budding yeast |
| RAD26 | RADiation sensitive 26 | Deletion of RAD26 has no effect on replicative lifespan in PSY316 [10207108]. | Budding yeast |
| RAD1 | RADiation sensitive 1 | Deletion of RAD1 has no effect on replicative lifespan [10207108]. | Budding yeast |
| PUF4 | PUmilio-homology domain Family 4 | Deletion of PUF4 has no effect on replicative lifespan in either uth4-14c (C-terminal truncation) or UTH4 background. However, PUF4 is required for lifespan extension by the semi-dominant Sir4-42 allele in the uth4-14c background [9150138].
PUF4 is required for nucleolar relocalization of Sir3 in a Sir4-42 background [9150138]. puf4;mpt5 double deletion strain has increased telomere silencing relative to the mpt5 single mutant [9651685]. | Budding yeast |
| POL1 | POLymerase 1 | Mutation of POL1 results in a 20-60% reduction in mean lifespan (in SS111) [12024027] | Budding yeast |
| NNT1 | Nicotinamide N-methylTransferase 1 | Deletion of NNT1 decreases mean and maximum lifespan by 9 and 19%. 0.5% glucose DR extends the mean and maximum lifespan of NNT1 deletion mutants by 35 and 40%. Overexpression of NNT1 by 5-fold extends mean and maximum replicative lifespan by 18 and 23%, which is approximately of the same magnitude as the lifespan extension obtained from DR. DR in NNT1 overexpression mutant fails to significantly affect the lifespan and only results in extended mean lifespan by 12% and reduced maximum lifespan by 11%. NNT1 overexpression increases rDNA silincing, whereas deletion decreases rDNA silencing. Overexpression of human nicotinamide N-methyltransferase also increases rDNA silencing [12736687]. | Budding yeast |
| NCA3 | Nuclear Control of ATPase 3 | Disruption in NCA3 shortens mean (87% of normal), nut not maximum replicative lifespan without causing any other gross changes in cell cycle parameters of growth characteristics [8810036].
In combination with an NCA2 disruption, NCA3 disruption causes a cryosensitive phenotype on non-fermentable carbon sources due to a defect in the F1-F0 ATP synthetase due to misbalancing of alternate spliceforms of mitochondrial mRNA encoding subunits 6 and 8 of the synthase [7586026]. | Budding yeast |
| HSC80 | — | Deletion of HSC82 has no effect on replicative lifespan, but shortens chronological lifespan [11361336]. | Budding yeast |
| HOG1 | High Osmolarity Glycerol response 1 | Deletion of HOG1 shortens replicative lifespan by 25% and prevents lifespan extension by high osmolarity [12391171]. HOG1 is required for many of the transcriptional responses to high osmolarity, including increased glycerol biosynthesis and MSN2/4-dependent stress response [10722658]. HOG1 deletion slightly decreases chronological lifespan and partially suppresses the premature aging phenotype and short lifespan of ISC1 deletion [22445853]. | Budding yeast |
| HAP5 | Heme Activator Protein 5 | Deletion of HAP5 shortens replicative lifespan by approximately 40%. This is not a premature aging phenotype as "old" HAP5 cells do not become premature sterile or exhibit other biomarkers of yeast aging [9271578]. HAP5 null mutants are unable to grow on a non-fermentable carbon source [7828851]. | Budding yeast |
| GPD1 | Glycerol-3-Phosphate Dehydrogenase 1 | GPD1 deletion shortens replicative lifespan by 25% and prevents lifespan extension by high osmolarity [12391171]. Transcripational regulation of GPD1 by osmotic stress requires HOG1 [8196651]. | Budding yeast |
| GAL83 | GALactose metabolism 83 | Deletion of GAL83 has no effect on replicative lifespan in S228C [10921902] and general GAL83 mutants have no obvious phenotype [10990457]. | Budding yeast |
| DNL4 | DNA Ligase 4 | DNL4 deletion does not affect lifespan [10521401], although it renders cells defective for non-homologous end-joining [9242411] | Budding yeast |
| CPR7 | Cyclosporin-sensitive Proline Rotamase 7 | Deletion of CPR7 has no effect on lifespan replicative lifespan, but increases chronological lifespan [11361336] | Budding yeast |
| COQ3 | COenzyme Q 3 | Deletion of COQ3 decreases chronological lifespan and renders cells respiratory deficient and sensitive to hydrogen peroxide [12586694]. | Budding yeast |
| CIT2 | CITrate synthase 2 | Deletion or overexpression of CIT2 has no effect on replicative lifespan. CIT2 is not required for the strain specific lifespan extension in petite cells [10224252], although it is transcribed up to 30-fold higher in petite cells relative to grande cells [1986232]. | Budding yeast |
| CDC7 | Cell Division Cycle 7 | Transient inactivation of CDC7 results in a shortened replicative lifespan [2698814]. CDC7 participates in silencing and RAS2 modulates its activity [1990268]. | Budding yeast |
| HPR1 | HyPerRecombination 1 | Deletion of HPR1 decreases replicative lifespan [11756539] | Budding yeast |
| OSH7 | OxySterol binding protein Homolog 7 | Overexpression of OSH7 extends mean replicative lifespan. PERG6-OSH7 does not extend the maximum lifespan significantly [Xia et al., unpublished].
Deletion of the CC domain of Osh7 (Perg6-OSH7-ORD) greatly shortens the lifespan. Deletion of the Osh7's CC domain decreases lifespan (Perg6-OSH7-ORD) shortens the lifespan [Tang et al., personal communication].
Osh7 interacts with the late endosome ATPase Vps4 by their C-terminal coiled-coil (CC) domain. | Budding yeast |
