NCA3, a nuclear gene involved in the mitochondrial expression of subunits 6 and 8 of the Fo-F1 ATP synthase of S. cerevisiae.

Authors: Pélissier P; Camougrand N; Velours G; Guérin M

Abstract: Respiratory-competent nuclear mutants have been isolated which presented a cryosensitive phenotype on a non-fermentative carbon source, due to a dysfunctioning of the mitochondrial F1-Fo ATP synthase which results from a relative defect in subunits 6 and 8 of the Fo sector. Both proteins are mtDNA-encoded, but the defect is due to the simultaneous presence of a mutation in two unlinked nuclear genes (NCA2 and NCA3, for Nuclear Control of ATPase) promoting a modification of the expression of the ATP8-ATP6 co-transcript (formerly denoted AAP1-OLI2). This co-transcript matures at a unique site to give two cotranscripts of 5.2 and 4.6 kb in length: in the mutant, the 5.2-kb co-transcript was greatly lowered. NCA3 was isolated from a wild-type yeast genomic library by genetic complementation. The level of the 5.2-kb transcript, like the synthesis of subunits 6 and 8, was partly restored in the transformed strain. A 1011-nucleotide ORF was identified that encodes an hydrophilic protein of 35417 Da. Disruption of chromosomal DNA within the reading frame promoted a dramatic decrease of the 5.2-kb mRNA but did not abolish the respiratory competence of a wild-type strain. NCA3 is located on chromosome IV and produces a single 1780-b transcript.

Keywords: Amino Acid Sequence; Base Sequence; Chromosome Mapping; Cloning, Molecular; Genes, Fungal; Mitochondria/*enzymology/*genetics/metabolism; Molecular Sequence Data; Mutation; Protein Biosynthesis; Proton-Translocating ATPases/*genetics; RNA, Messenger; Saccharomyces cerevisiae/*enzymology/*genetics; Sequence Analysis, DNA; Transcription, Genetic
Journal: Current genetics
Volume: 27
Issue: 5
Pages: 409-16
Date: April 1, 1995
PMID: 7586026
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Citation:

Pélissier P, Camougrand N, Velours G, Guérin M (1995) NCA3, a nuclear gene involved in the mitochondrial expression of subunits 6 and 8 of the Fo-F1 ATP synthase of S. cerevisiae. Current genetics 27: 409-16.



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