| HAP5 deletion | Deletion of HAP5 shortens replicative lifespan by approximately 40%. This is not a premature aging phenotype as "old" HAP5 cells do not become premature sterile or exhibit other biomarkers of yeast aging [9271578]. HAP5 null mutants are unable to grow on a non-fermentable carbon source [7828851]. | Yeast | -40 | — | — |
| HDA1 deletion | Deletion of HDA1 has no effect on longevity under AL, but acts synergistically with 0.1% glucose restriction to increase replicative lifespan [12213553]. Deletion of HDA1 leads to a slightly increased chronological lifespan [19801973]. Deletion of HDA1 has no effect on the wild-type lifespan in the short-lifespan of YSK771 strain, but suppresses the short-lifespan of SIR3 mutants [10512855]. Null mutation results in increased telomeric silencing and increased histone acetylation [8962081]. | Yeast | — | — | — |
| YKU70 deletion | Deletion fo YKU70 shortens lifespan, but does not accelerate the normal aging process [10521401]. YKU70 null mutants are defective for non-homologous end-joining [8754818] and for telomeric silencing [9635192]. | Yeast | — | — | — |
| YKU80 deletion | Deletion of YKU80 shortens replicative lifespan, but does not accelerate the normal aging process [10521401]. YKU80 null mutant is defective for non-homologous end-joining [8754818] and for telomere silincing [9501103; 9635192] | Yeast | — | — | — |
| HOG1 deletion | Deletion of HOG1 shortens replicative lifespan by 25% and prevents lifespan extension by high osmolarity [12391171]. HOG1 deletion slightly decreases chronological lifespan and partially suppresses the premature aging phenotype and short lifespan of ISC1 deletion [22445853]. | Yeast | -25 | — | — |
| SRS2 deletion | Deletion of SRS2 shortens mean replicative lifespan by 50% [11290710]. Overexpression of SGS1 increases maximum, but not mean lifespan of SRS2 mutants [11861900]. Deletion of SRS2 is synthetical lethal in combination with deletion of SGS1 [11290710]. | Yeast | -50 | — | — |
| HSC82 deletion | Deletion of HSC82 has no effect on replicative lifespan, but shortens chronological lifespan [11361336]. | Yeast | — | — | — |
| HSP104 deletion | Deletion of HSP104 leads to a 14% [9851879] to 40% [17908928] reduction in mean replicative lifespan, therfore it is required for required for longevity. Exposure of cells to transient sub-lethal heat-stress extends mean lifespan by 12% while decreasing maximum lifespan by 14%. This effect does not occur in an HSP104 null mutant [9851879]. HSP104 null mutant is viable but displays reduced high temperature survival [8643570]. | Yeast | -14 to -40 | — | — |
| IDH2 deletion | Deletion of IDH2 increases the mean replicative lifespan by about 30% [16293764]. IDH2 deletion extends mean replicative lifespan by 20% in the alpha strain and in a strain [19030232; 18340043]. IDH2 deletion extends mean, median and maximum lifespan by 15, 19 and 15% [23167605]. | Yeast | +15.4 to +30 | +19.2 | +15.4 |
| LAC1 deletion | Deletion of LAC1 the homolog of LAG1 [9872981] has no effect on replicative lifespan in strain W303R and PSY316 [N. Bishop, G. Liszt, and L. Guarente, unpublished]. LAC1 null mutant has no obvious growth defect [10198056], but is synthetically lethal with LAG1 mutation [9872981]. | Yeast | — | — | — |
| LAG1 deletion | A gene deletion of LAG1 in haploid cells results in a pronounced increase (approximately 50%) in mean and in maximum replicative lifespan in the YPHDF-1A strain [8195187], but has no significant effect on lifespan in stains W303R or PSY316 (N. Bishop, G.Liszt, and L. Guarente, unpublished]. The LAG1 transcribed is preferentially expressed in young cells. LAG1 null mutant is viable and has no obvious phenotypes but shows delayed ER to Golgi transport when combined with DGT1 mutation [10198056] and is synthetical lethal with LAC1 deletion. | Yeast | +50 | — | +50 |
| LAG2 deletion | Deletion of LAG2 in haploid SP1 strain does not affect growth, but results in a 50% decrease in the mean and maximum replicative lifespan [8760941]. | Yeast | -50 | — | -50 |
| Mating type heterozygosity | Mating type heterozygosity results in an approximately 20% mean replicative lifespan reduction, which occurs in both haploid and diploid cells (W303R) [10521401] and is likely due to increased homologous recombination [8454201] and rDNA circle formation [10521401]. | Yeast | -20 | — | — |
| MPT5 deletion | Deletion of MPT5 shortens replicative lifespan by about 50% [9150138; 7859289]. MPT5 deletion decreases average chronological lifespan by 50%, which is rescued to the wild-type level by PKC1 overexpression [17172436].
MPT5 mutants are temperature sensitive [7845352], hypersensitive to mating pheromone [9154842], and null mutants exhibit increased silencing at telomeres and decreased rDNA silencing [9584615]. Deletion of MPT5 is synthetical lethal with mutation of either SWI4, SWI6, or CCR4 in an ssd1-d background [11805047]. MPT5 is required for relocalization of the SIR complex to the nucleolus in sir4-42 strain [7859289]. | Yeast | -50 | — | — |
| MSN2 MSN4 double mutation | Deletion of MSN2 and MSN4 extends replicative lifespan and is further extended by cyr1::mTn [14741356]. Deletion of MSN2 and MSN4 does not significantly decrease chronological lifespan under AL, but attenuates chronological lifespan extension by water starvation and 0.5% glucose restriction [18225956] as well as cancels out lifespan extension of cyr1::mTn [14741356] and decreases chronological lifespan extension of ras2 deletion mutant [12586694]. Simulatnous deletion of MSN2 and MSN4 has no effect on chronological lifespan, but prevents lifespan extension by RAS2 deletion [12586694]. msn2 msn4 has no effect on replicative lifespan in PSY316, and does not prevent lifespan extension by DR [11000115] or by high osmolarity [12391171]. | Yeast | — | — | — |
| NCA3 deletion | Disruption in NCA3 shortens mean (87% of normal), nut not maximum replicative lifespan without causing any other gross changes in cell cycle parameters of growth characteristics [8810036].
In combination with an NCA2 disruption, NCA3 disruption causes a cryosensitive phenotype on non-fermentable carbon sources due to a defect in the F1-F0 ATP synthetase due to misbalancing of alternate spliceforms of mitochondrial mRNA encoding subunits 6 and 8 of the synthase [7586026]. | Yeast | -87 | — | — |
| DNM1 deletion | Deletion of DNM1 extends significantly mean and maximum lifespan by 49 and 111% in FY10 strain and by 15 and 12% in BY4741 strain [17173038]. | Yeast | +15 to +49 | — | +12 to +111 |
| FIS1 deletion | Deletion of FIS1 prolongs significantly mean and maximum lifespan by 13 and 29% as well as improves the fitness of old mother cells (in BY4741) [17173038]. | Yeast | +13 | — | +29 |
| NMT1 mutation | nmt1-451D allele shortens mean and maximum replicative lifespan by 47 and 44% at 24 degree Celsius (permessive temperature). Mutants have decreased resistance to acute and gradual nutrient deprivation, even at the permissive temperature [10921902]. A NMT null mutant is lethal. | Yeast | -47 | — | -44 |
| NNT1 deletion | Deletion of NNT1 decreases mean and maximum lifespan by 9 and 19%. 0.5% glucose DR extends the mean and maximum lifespan of NNT1 deletion mutants by 35 and 40%. NNT1 deletion decreases rDNA silencing [12736687]. | Yeast | -9 | — | -19 |
| NPT1 deletion | NPT1 deletion decreases replicative lifespan by 50% [17482543] as well as chronological lifespan [17110466]. Deletion of NPT1 shortens the lifespan in W303R. Replicative lifespan extension of cdc25-10 mutation (assumed to act as a genetic DR-mimetic) is cancelled out by NPT1 deletion [11000115].
NPT1 mutation results in loss of telomere and rDNA silencing [10841563], an effect that is likely caused by a loss of SIR2 activty due to decreased NAD levels. Mutation of NPT1 is synthetical lethal with mutation of QPT1 [11000115]. | Yeast | -50 | — | — |
| Petite | Respiratory deficient petite cells in YPK9 strain have a replicative lifespan that is extended by 39%. This lifespan extension is strain-specific as Petite cells in W303 have lifespan identical to grande cells and petite cels in strains SP1-1 and A364A have a shorter lifespan than grande cells [10224252].
Respiratory defective petite cells display defective mitochondria and are unable to grow on glycerol. | Yeast | +39 | — | — |
| PHB1 deletion | Deletion of PHB1 results in a slight reduction in mean and maximum replicative lifespan and a defect in mitochondrial membrane potential. When both PHB1 and PHB2 genes are deleted, the mean replicative lifespan is reduced by one third (30%) that of the wild-type strain [9259555]. Deletion of PHB1 decreases replicative lifespan by 20% [12882345]. Phenotypic changes characteristic of aging cells (e.g. lengthening of cell cycle and specific morphological changes) suggests that PHB1;PHB2 double mutants undergo premature aging, not simply reduction of viability [9259555].
There is no reduction in stress resistance or bulk growth rate in PHB1 mutants. PHB1;PHB2 double mutant have a strong defect in mitochondrial potential, while PHB1 mutant have only a slight defect [9259555]. PHB1 deletion is synthetical lethal with mutation of outer mitochondrial membrane proteins, Mdm12, Mdm10, or Mmm1 [9632789]. | Yeast | -30 | — | — |
| PHB2 deletion | PHB2 deletion leads to a slight reduction in both mean and maximum replicative lifespan, and when both PHB1 and PHB2 genes are deleted, the mean replicative lifespan is reduced by 40% [9259555]. Deletion of PHB2 decreases replicative lifespan by 30% [12882345]. Phenotypic changes characteristic of aging cells (e.g. lengthening of cell cycle and specific morphological changes) suggests that PHB1;PHB2 double mutants undergo premature aging, not simply reduction of viability [9259555].
PHB2 mutants exhibit no reduction in stress resistance or bulk growth rate. PHB1;PHB2 double mutant have a strong defect in mitochondrial potential [9259555].
Prohibitin-dependent mutation pbd1 and pdb2 behave in a different manner and probaly affect different aspects of prohibitin function. pdb1 mutants slightly extended lifespan by 11%, whereas in contrast, the pdb2 mutation results in a shortening in both the mean- and the maximum-lifespan (by 28 and 17%, respectively). pdb1 mutation also reduces chronological lifespan. Reducing the expression of the PHB2 in the pbd mutants retards the rate of growth and affects replicative lifespan [16710639]. | Yeast | -30 | — | — |
| PNC1 deletion | Deletion of PNC1 shortens replicative lifespan approximately by 10% [12736687] and largely prevents replicative lifespan extension of 0.5% glucose restriction. 0.5% glucose restriction slightly extends median replicative lifespan (by 10 - 15%) but not maximum replicative lifespan in pnc1Delta [14724176]. PNC1 deletion decreases chronological lifespan [17110466]. | Yeast | -10 | — | — |
GenAge
GenDR
