| CLN3 overexpression | Overexpression shortens chronological lifespan together with age-dependent increases in genome instability and apoptosis. While around 80% of wild-type cells are alive almost non CLN3 overexpressers are alive (under condition that avoids adaptive regrowth) [17710147]. | Yeast | — | — | — |
| concA treatment | The specific V-ATPase inhibitor concanatmycin A (concA) blocks VMA1 or VPH2 overexpression mutations ability to produce normal, tubular mitochondria. Treatment of young cells causes vacuolar acidity and loss of mitochondrial depolarization. Loss of ΔΨ is followed by mitochondrial fragmentation and aggregation that resembles mitochondrial phenotypes present in aged cells [23172144]. | Yeast | — | — | — |
| COQ3 deletion | Deletion of COQ3 decreases chronological lifespan and renders cells respiratory deficient and sensitive to hydrogen peroxide [12586694]. | Yeast | — | — | — |
| CPR7 deletion | Deletion of CPR7 has no effect on lifespan replicative lifespan, but increases chronological lifespan [11361336] | Yeast | — | — | — |
| CRC1 deletion | Deletion of CRC1 increases replicative lifespan [16293764]. CRC1 deletion increases replicative lifespan by 25% in the alpha strain [19030232] | Yeast | +25 | — | — |
| CSR2 deletion | Deletion of CSR2 results in higher levels of intracellular reactive oxygen species, augmentation of scavenging enzymes and extends replicative as well as chronological lifespan [16336970]. | Yeast | — | — | — |
| CTA1 Deletion | Deletion of CTA1 increases chronological lifespan [21076178]. | Yeast | — | — | — |
| CTA1 overexpression | CTA1 overexpression partially suppresses the shortened chronological lifespan by ISC1 mutation [21707788]. | Yeast | — | — | — |
| CTF4 deletion | Deletion of CTF4 results in an approximately 75% reduced mean replicative lifespan [12024027]. | Yeast | — | — | — |
| CTF8 deletion | Deletion of CTF8 increases mean chronological lifespan by 15-92% (15, 18, 42, 45, 92) in diploid cells [21447998]. | Yeast | +15 to +92 | — | — |
| CTT1 deletion | Deletion of CTT1 confers longer chronological lifespan [21076178]. | Yeast | — | — | — |
| CTT1 mutation | Mutational inactivation of CTT1 increases chronological lifespan [20696905]. | Yeast | — | — | — |
| CTT1 overexpression | Overexpression of cytosolic catalase T CTT1 alone slightly shortens stationary phase survival in strain DBY746. Overexpression CTT1 in combination with SOD1 increases stationary phase survival by about 10% [12586694]. | Yeast | — | — | — |
| CUP9 deletion | Deletion of CUP9 increases replicative lifespan by 30% in the alpha and a strains [18340043]. Although CPU9 was identified as a potential long-lived mutant strain in a bar-code screen, the chronological lifespan of CUP9 deletion mutant is not significantly different from than of wild-type under starvation/extreme DR [20657825]. | Yeast | — | — | — |
| CYR1 mutation | The CDC35-1 allele of the adenylate cyclase CYR1 confers a 75% extension of replicative lifespan at 25 degree Celsius [11000115]. cyr1-1 mutation extends median chronological lifespan by 28-47% and is non-addative with lifespan extension conferred by overxpression of human MAPK1 [17662940]. | Yeast | +75 | +28 to 47 | — |
| CYR1 transposition | Transposon-mutagenized CYR1 increases resistance to oxidants and extends chronological lifespan by up to 90%. Stress-resistance transcription factors Msn2/Msn4 and protein kinase Rim15 are required for this lifespan extension [11292860]. | Yeast | +300 | — | — |
| CYT1 deletion | Deletion of CYT1 increases replicative lifespan by 15% in the alpha strain and decreases replicative lifespan by 20% in a strain. Deletion of CYT1 decreases replicative lifespan and cancels out replicative lifespan extension by HAP4 overexpression. Initially, it was shown that deletion of CYT1 also prevents lifespan extension by 0.5% glucose restriction [12124627], but later it was shown that either 0.5 or 0.05 % glucose restriction increases replicative lifespan of cyt1Delta cells [16311627]. | Yeast | — | — | — |
| CYT2 mutation | Mutation of CYT2 decreases replicative lifespan by 25% in the alpha strain [18340043; 19030232]. | Yeast | -25 | — | — |
| D-glucosamine treatment | Addition of 0.5 mg/ml D-glucosamine to the growth media suppresses the short replicative lifespan and temperature sensitive growth of mpt5 mutant, but fails to extend the lifespan of wild-type cells [11805047]. | Yeast | — | — | — |
| DAP2 deletion | DAP2 deletion decreases mean and maximum replicative lifespan under AL by 19 and 36%, respectively, and cancels out the lifespan extending effect of moderate DR [22912585]. | Yeast | -19 | — | -36 |
| Deletion of mitochondrial DNA | Activation of the retrograde response by deletion of mitchondrial DNA (rho0) extends mean and maximum replicative lifespan by 24 - 51% and 15 - 65%, respectively [10224252]. Lifespan extension associated with impaired mitochondria depends on a retrograde intracellular signalling involving at leas three transcription factors, which adjust nuclear gene expression an induce shift of metabolism from Krebs cycle to the glyoxylate cycle [Refs 41,42 i Lee et al., 2002]. | Yeast | +24 to +51 | — | +15 to +65 |
| Diazaborine | Treatment of wild-type cells with 15 microgram/ml diazaborine extends mean (24.7 -> 36.9) and maximum replicative lifespan [18423200]. | Yeast | — | — | — |
| DIF1 deletion | Deletion of DIF1 decreases replicative lifespan by 20% in the alpha strain [19030232]. | Yeast | -20 | — | — |
| DNA2 deletion | Mutants in DNA2 exhibit an accelerated ageing phenotype including extended cell cycle time, age-related transcriptional silencing defects, and nucleolar reorganization, which are all phenotypes of old wild-type cells. Lifespan of DNA2 mutants is extended by expression of an additional copy of SIR2 or by deletion of FOB1 and therefore the lifespan shortening partially suppressed. Three different alleles of DNA2 (dna2-1, dna2-2, and dna2-20) result in a significant shortened lifespan up to 85%. DNA2 mutation shorten the already short lifespan of SGS1 mutants [12024027]. | Yeast | — | — | — |
| DNL4 deletion | DNL4 deletion does not affect lifespan [10521401], although it renders cells defective for non-homologous end-joining [9242411] | Yeast | — | — | — |
GenAge
GenDR
