| REI deletion | REI1 deletion increases mean replicative lifespan by about 40% [16293764] in the alpha and a strains [19030232]. | Yeast | +40 | — | — |
| GUP1 deletion | GUP1 deletion extends mean and maximum replicative lifespan by 32 and 30%, respectively, as well as chronological lifespan. DR-induced maximal replicative lifespan extension is not further increased by GUP1 deletion, while gup1 mutant displayed longer chronological lifespan under DR [21584246]. | Yeast | +32 | — | +30 |
| High Glucose | High glucose concentration decreases chronological lifespan [21076178]. | Yeast | — | — | — |
| AIM4 deletion | AIM4 (alias SOY1) deletion increases chronological and replication lifespan, which is non-additive with DR. On AL mean and maximum replicative lifespan are extended by 63 and 69%, respectively. DR appears to decrease aim4-induced replication lifespan extension, indicating a negative interaction. aim4 mutation does not change DR-induced chronological lifespan extension [21584246]. | Yeast | +63 | — | +69 |
| N-acetylcysteine Treatment | Treatment with N-acetylcysteine decreases chronological lifespan [21076178]. | Yeast | — | — | — |
| HHF1 deletion | HHF1 deletion extends mean and maximum replicative by 45 and 69%, respectively, as well as chronological lifespan. Chronological lifespan extension by HHF1 deletion and DR is non-synergistic. DR appears to extend replicative lifespan more when combined with hhf1 mutation, whereas DR does not change hhf1-induced replicative lifespan extension, suggesting a positive interaction [21584246]. | Yeast | — | — | — |
| ROM2 deletion | Deletion of ROM2 increases mean replicative lifespan of the alpha strain by about 50% [16293764]. ROM deletion mutant replicative lifespan increases by 49% in the alpha strain and 16% in a strain. Deletion of ROM2 increases replicative lifespan by 38% in the alpha strain and by 29.3% in the a strain (34.2% in both) [19030232]. | Yeast | +16 to +50 | — | — |
| SFA1 deletion | Deletion of SFA1 alone has no effect on replicative lifespan. sfa1;yhb1 double mutant cancels out the ability of moderate DR to extend replicative lifespan, but not chronological lifespan. Yhb1 and Sfa1 may play redundant roles hb1 and Sfa1 may play redundant roles [21584246]. | Yeast | — | — | — |
| RPD3 deletion | Deletion of the histone deacetylase gene RPD3 extends lifespan by 41%, independently of an intact Sir silencing complex (in the short lived YSK661 strain) [10512855]. Deletion of RPD3 extends replicative lifespan and there was no additive effect by neither 0.1% glucose nor amino acid restriction [12213553].
RPD3 deletion increases rDNA silencing in a partially SIR2-dependent manner [10082585].
Its effects on chromatin functional state were evidenced by enhanced silencing at the three known heterochromatic regions in the genome, the silent mating type (HM), subtelomeric, and rDNA loci, which occurred even in the absence of SIR3 [10512855]. | Yeast | +41 | — | — |
| YHB1 deletion | Deleting YHB1 partially abolished DR-induced replicative lifespan extension, whereas deleting SFA1 alone had no effect. sfa1;yhb1 double mutant cancels out the ability of moderate DR to extend replicative lifespan, but not chronological lifespan. Yhb1 and Sfa1 may play redundant roles [21584246]. | Yeast | — | — | — |
| SIR3 deletion | Deletion of SIR3 shortens replicative lifespan by approximately 20% [10521401]. SIR3 mutants exhibit a loss of silencing at the silent mating loci [6098447; 3297920] and telomerease [1913809] and have a slighlty elevuated level of rDNA marker loss [10521401]. The lifespan reduction of SIR3 deletion is suppressed by preventing mating type heterozygosity and is therefore probably due to the simultaneous expression of a and alpha mating-type information, which indirectly causes an increase in rDNA recombination and likely increases the production of extrachromosomal rDNA circles [10521401]. Deletion of SIR3 itself has little effect on lifespan, although it markedly accelerates the increase in cell generation time that occurs during aging [10512855]. | Yeast | -30 | — | — |
| SFA1 YHB1 double mutation | sfa1;yhb1 double mutant cancels out the ability of moderate DR to extend replicative lifespan, but not chronological lifespan. Indicating that NO homeostasis during DR-induced replicative lifespan extension is crucial. Deleting YHB1 partially abolished DR-induced replicative lifespan extension, whereas deleting SFA1 alone had no effect. Yhb1 and Sfa1 may play redundant roles [21584246]. | Yeast | — | — | — |
| RPL31A deletion | Deletion of RPL31A increases mean replicative lifespan by 45% [16293764]. Mean replicative lifespan increases by 35% in the alpha strain and 50% in a strain [19030232; 18423200]. Mean replicative lifespan of the RPL31A deletion mutant increases by 35% in the ORF collection and by 29% in the remade strain [22377630]. RPL31A deletion increases significantly replicative lifespan [17174052]. Deletion of RPL31A extends replicative lifespan and is not further extended by 0.05% glucose restriction [18423200]. | Yeast | +29 to +50 | — | — |
| VPS20 deletion | VPS20 deletion decreases mean and maximum replicative lifespan by 16% and 19%, respectively, and additionally cancels out the DR-induced replicative lifespan extension [22912585]. | Yeast | -16 | — | -19 |
| RPL6A deletion | Deletion of RPL6A decrease mean replicative lifespan by 25% in the alpha strain [18340043; 18423200], but increases mean replicative lifespan by 40% in the remade strain. Its deletion non-significantly increases mean replicative lifespan in the ORF collection [22377630]. | Yeast | -25 to +40 | — | — |
| FRE6 deletion | FRE6 deletion increases mean replicative lifespan by 14% and cancels out the lifespan extending effect of DR [22912585]. | Yeast | +14 | — | -2 |
| RPL6B deletion | Deletion of RPL6B significantly increases replicative lifespan [17174052]. Replicative lifespan increases by 15% in the alpha strain and 30-40% in a strain [19030232;18340043;18423200]. RPL6B deletion increases replicative lifespan by 30% [16293764]. | Yeast | +15 to +40 | — | — |
| RCR2 deletion | RCR2 deletion extends mean replicative lifespan by 18% and cancels out the lifespan extending effect of DR [22912585]. | Yeast | +18 | — | -4 |
| RSR1 deletion | Deletion of RSR1 (alias BUD1) shortens replicative lifespan [9789734]. | Yeast | — | — | — |
| YDL180W deletion | YDL180W deletion impairs DR-mediated replicative lifespan extension, but does not change lifespan on AL significantly [22912585]. | Yeast | +5 | — | -6 |
| BCY1 deletion | Disruption in BCY1 by mutation results decreases mean and maximum replicative lifespan by 37 and 16% and is associated with increased PKA activity [8195187]. | Yeast | -37 | — | -16 |
| RTG2 deletion | RTG2 is required for replicative lifespan extension associated with the retrograde response, a pathway that signals the functional status of mitochondria to the nucleus to regulate the expression of several genes [11024000]. RTG2 is not required for replicative lifespan extension by DR [11024000].
RTG2 null mutants are not petite [8422683], but display various nutrient auxotrphies and alterations of carbohydrate metabolism [7727418]. | Yeast | — | — | — |
| OPT2 deletion | OPT2 deletion increases mean and maximum replicative lifespan by 23 and 9%, respectively, and cancels out the lifespan-extending effect of DR [22912585]. | Yeast | +23 | — | +9 |
| RTG3 deletion | RTG3 deletion mutation causes an increase in mean replicative in lifespan by 55% increase at 2% glucose, suggesting that expression of genes regulated by Rtg1-Rtg3 has a negative effect on longevity in 2% glucose (in YPK9). A null mutant has 123% increased mean lifespan at 0.1% glucose relative to a wild-type strain at 2% glucose, indicating that reduced glucose and an RTG3 mutation have an additive effect on lifespan (in YPK9) [11024000].
RTG3 null mutant cannot grow on acetate medium and requires glutamate or aspartate for growth [9032238]. | Yeast | +55 | — | — |
| GTR1 deletion | GTR1 deletion decreases mean and maximum replicative lifespan under AL by 36 and 51%, respectively, and cancels out the lifespan extending effect of DR [22912585]. | Yeast | -36 | — | -51 |
GenAge
GenDR
