| HAC1 deletion | Deletion of HAC1 decreases mean, median and maximum replicative lifespan by 10, 8 and 5%, respectively [23167605]. | Yeast | +10.3 | +8.3 | +5.3 |
| Ilp2 mutation | Ilp2 null mutants are significant longer-lived with a 8-13% longer median lifespan [20195512]. | Fly | — | +8 to +13 | — |
| elo-2 RNAi | elo-1 RNAi in the adulthood extends lifespan by 8% [21386131]. | Worm | — | +8 | — |
| ubh-4 RNAi | RNAi of ubh-4 starting at L1 extends mean, 25%ile and median lifespan by 3, 8 and 8% [15998808]. | Worm | +3 | +8 | — |
| rnf-5 RNAi | RNAi of rnf-5 starting at L1 extends mean, 25%ile and median lifespan by 3, 8 and 8% [15998808]. | Worm | +3 | +8 | — |
| C18E9.4 RNAi | RNAi of C18E9.4 starting at L1 results in mean and median lifespan extension by about 4 and 8% [15998808]. | Worm | +4 | +8 | — |
| inx-14 RNAi | RNAi of inx-14 starting at L1 increases mean, 25%ile and median lifespan by 6, 8 and 14% [15998808]. | Worm | +6 | +8 | — |
| F35D2.3 RNAi | RNAi of F35D2.3 extends mean, 25%ile and median lifespan by 3, 8 and 8% [15998808]. | Worm | +3 | +8 | — |
| F40F8.5 RNAi | RNAi of F40F8.5 extends mean, 25%ile and median lifespan by 3, 8 and 8% [15998808]. | Worm | +3 | +8 | — |
| drr-1 RNAi | RNAi of drr-1 starting at L1 extends mean, 25%ile and median lifespan by 3, 8 and 8% [15998808]. drr-1 RNAi significantly extends mean lifespan of wild-type by 37 - 44%, of daf-16 mutants by 18%, and of daf-2 mutants by 14%, but does not extend significantly the lifespan of eat-2 mutants. drr-1 RNAi does not affect pumping, but similar to DR reduces and delays reproduction as well as causes a slender appearance. drr-1 mRNA is 2-fold downregulated in response to DR [16103914]. | Worm | +3 to +44 | +8 | — |
| spt-4 RNAi | RNAi of spt-4 starting at L1 extends mean, 75%ile and median lifespan by 4, 8 and 8% [15998808]. | Worm | +4 | +8 | — |
| mecr-1 RNAi | RNAi of mecr-1 extends mean, median and 25%ile lifespan by 6, 8 and 8% [15998808]. | Worm | +6 | +8 | — |
| snz mutation | Mutation in snz increases maximum lifespan of both sexes by up to 66%, while the median female lifespan is approximately 85% higher and that of males around 72% [18478054]. | Fly | — | +72 to +85 | +66 |
| MSRA overexpression | Animals engineered to overexpress bovine MSRA in the nervous system have an extended median lifespan by up to 70% (relative to parental control), increased resistance to oxidative stress, and delayed the onset of senescence-induced decline in activity levels and reproductive capacity [11867705]. | Fly | — | +70 | — |
| Resveratrol supplementation | A maximum dose of resveratrol increases the median lifespan by 56% [16461283]. | Fish | — | +56 | — |
| Orco mutation | Loss-of-function mutation in Orco (alias Or83b) results in olfactory defects, altered adult metabolism, enhanced stress resistance, and life-extension. Fully fed female homozygous Or83b null mutants exhibit a 56% increase in median lifespan and a 30% increase in maximum lifespan. Males are also significantly longer-lived, though to a smaller degree and maximum lifespan is not extended. Heterozygous mutants of both sexes show an intermediate longevity. Lifespan of homozygous Or83b null mutants is further increased by DR, but the relative increase in median and mean longevity is significantly greater when mutants were maintained in well-fed conditions [17272684]. | Fly | — | +56 | +30 |
| crtc-1 RNAi | RNAi of crtc-1 extended median lifespan by up to 53% [21331044]. | Worm | — | +53 | — |
| OSH6 overexpression | Elevation of OSH6 levels by an ERG6 promoter extends mean, median and maximum replicative lifespan by 39, 52 and 18% which is non-additive with 0.5% glucose restriction. It also extends the lifespan of NYV1 mutant [Geber et al., unpublished].
The long lifespan of Perg6-OSH6 is not further extended by deletion of TOR1 [22622083].
OSH6 overexpression decreases total cellular sterol content and reduces Lst8 protein levels. The CC domain of Osh6 is dispensable for longevity [Fusheng Tang, personal communication]. | Yeast | +39 | +52 | +18 |
| hep overexpression | Overexpression of hey significantly extends median (50%) and maximum (25%) lifespan [14602080]. | Fly | — | +50 | +25 |
| Rapamycin treatment | Treatment of Drosophila imago with rapamycin induces increases of median (by 5-6%) lifespan (p < 0.01) in males and females, respectively and increase of maximum lifespan (by 33%) in females (p < 0.01) [22661237].
Low dose of LY294002 (5 microM) slightly increase the median and maximum lifespan [20017609]. | Fly | — | +5 to +6 | +33 |
| Wortmannin treatment | Treatment of Drosophila imago with 0.5 micromolar wortmannin increases median (by 5%) and maximum (by 39%) lifespan in males (p < 0.001), but the lifespan differences in females were statistical insignificant (p > 0.05) [22661237].
Low dose of wortmannin (5 microM) slightly increase the median and maximum lifespan [20017609]. | Fly | — | +5 | +39 |
| Homozygous chico mutation | Mutation in chico extends mean, median, and maximum lifespan by 56%, 48%, and 42% in homozygotes. chico mutation produces dwarf, long-lived females at normal nutrition [11292874]. Wild-type and chico mutant females have similar peak lifespan under DR, but the food concentration at which these are achieved is shifted to higher amounts. chico mutation induces a state equivalent to submaximal, DR-induced slowing of aging [11951037]. Male chico homozygous have a shortened lifespan [11292874]. Female chico homozygous recessive mutants are sterile [11292874]. | Fly | +56 | +48 | +42 |
| aakg-2 overexpression | Overexpression of aakg-2 extends mean, median, and maximum lifespan by 47, 45, and 35% [22737090]. | Worm | +47 | +45 | +35 |
| RPS6B deletion | RPS6B deletion increases mean replicative lifespan by about 30% [16293764]. Deletion of RPS6B, but not of the RPS6A paralog increases replicative median lifespan robustly by 45% [17174052]. | Yeast | +30 | +45 | — |
| vps-37 RNAi | RNA interference of vps-37 decreases median lifespan by 44% in wild-type animals, 47% in a daf-2 background and 33% in daf-2/daf-16 double mutants [18006689]. | Worm | — | +44 | — |
GenAge
GenDR
