Authors: Tsuda M; Sugiura T; Ishii T; Ishii N; Aigaki T
Abstract: Oxidative stress is thought to promote aging by damaging various cellular components. We investigated the phenotypic effects of mutation in the sdhC gene encoding succinate dehydrogenase C in Drosophila by using transgenic flies expressing a dominant-negative form, SdhC(I71E). Expression of SdhC(I71E) significantly reduced the mean lifespan by 22% compared to that of control flies. The amount of protein carbonyl was significantly increased, suggesting that a high level of oxidative stress was induced in these flies. The transgenic flies should serve as a model for studying the biological processes associated with increased levels of oxidative stress and mitochondrial dysfunction in Drosophila.
Keywords: Animals; Bacterial Proteins/genetics/*metabolism; Caenorhabditis elegans Proteins/*metabolism; Drosophila/*physiology; Longevity/*physiology; Oxidative Stress/*physiology; Succinate Dehydrogenase/*metabolism
Journal: Biochemical and biophysical research communications Volume: 363 Issue: 2 Pages: 342-6 Date: Sept. 15, 2007 PMID: 17854771 |
Tsuda M, Sugiura T, Ishii T, Ishii N, Aigaki T (2007) A mev-1-like dominant-negative SdhC increases oxidative stress and reduces lifespan in Drosophila. Biochemical and biophysical research communications 363: 342-6.
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