Symbol: top-1 Name: TOPoisomerase Entrez gene ID: 266847 Ensembl gene ID: M01E5.5 Species: Worm (Taxid: 6239) Functional description: DNA topoisomerase I; Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). [UniProt] Observation: RNA interference decreased median lifespan by 19% in wild type animals and 42% in daf-2 mutants. Assays: Classification: Aging Relevance Analysis/Source: Homologs Edit \ Update (Admin) | Delete |
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