The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity.
Authors: Vandamme, Julien; Lettier, Gaelle; Sidoli, Simone; Di Schiavi, Elia; Norregaard Jensen, Ole; Salcini, Anna Elisabetta Year: 2012 Journal: PLoS Genet Abstract: Epigenetic modifications influence gene expression and provide a unique mechanism for fine-tuning cellular differentiation and development in multicellular organisms. Here we report on the biological functions of UTX-1, the Caenorhabditis elegans homologue of mammalian UTX, a histone demethylase specific for H3K27me2/3. We demonstrate that utx-1 is an essential gene that is required for correct embryonic and postembryonic development. Consistent with its homology to UTX, UTX-1 regulates global levels of H3K27me2/3 in C. elegans. Surprisingly, we found that the catalytic activity is not required for the developmental function of this protein. Biochemical analysis identified UTX-1 as a component of a complex that includes SET-16(MLL), and genetic analysis indicates that the defects associated with loss of UTX-1 are likely mediated by compromised SET-16/UTX-1 complex activity. Taken together, these results demonstrate that UTX-1 is required for many aspects of nematode development; but, unexpectedly, this function is independent of its enzymatic activity. Reference
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Created on Nov. 5, 2012, 5:58 p.m. Not linked Integrated: False
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