| CYT1 deletion | Deletion of CYT1 increases replicative lifespan by 15% in the alpha strain and decreases replicative lifespan by 20% in a strain. Deletion of CYT1 decreases replicative lifespan and cancels out replicative lifespan extension by HAP4 overexpression. Initially, it was shown that deletion of CYT1 also prevents lifespan extension by 0.5% glucose restriction [12124627], but later it was shown that either 0.5 or 0.05 % glucose restriction increases replicative lifespan of cyt1Delta cells [16311627]. | Yeast | — | — | — |
| DNA2 deletion | Mutants in DNA2 exhibit an accelerated ageing phenotype including extended cell cycle time, age-related transcriptional silencing defects, and nucleolar reorganization, which are all phenotypes of old wild-type cells. Lifespan of DNA2 mutants is extended by expression of an additional copy of SIR2 or by deletion of FOB1 and therefore the lifespan shortening partially suppressed. Three different alleles of DNA2 (dna2-1, dna2-2, and dna2-20) result in a significant shortened lifespan up to 85%. DNA2 mutation shorten the already short lifespan of SGS1 mutants [12024027]. | Yeast | — | — | — |
| FOB1 deletion | Mutation in FOB1 extends replicative lifespan by 30-50% [10230397]. FOB1 mutation increases replicative lifespan by 25% in the alpha strain and by 10% in a strain [19030232]. FOB1 mutant exhibit an about 20% mean replicative lifespan increase [15722108]. Deletion of FOB1 causes extension in the short life span of the sir2 mutant by around 50% [10521401]. Mutation of the FOB1 gene slows the generation of rDNA circles and thus extends life span by approximately 30% in W303 and 50% in K2307 [10230397]. Even in cells lacking both Sir2 and Fob1, nicotinamide prevents the lifespan extension by DR [16311627]. | Yeast | — | — | — |
| HDA1 deletion | Deletion of HDA1 has no effect on longevity under AL, but acts synergistically with 0.1% glucose restriction to increase replicative lifespan [12213553]. Deletion of HDA1 leads to a slightly increased chronological lifespan [19801973]. Deletion of HDA1 has no effect on the wild-type lifespan in the short-lifespan of YSK771 strain, but suppresses the short-lifespan of SIR3 mutants [10512855]. Null mutation results in increased telomeric silencing and increased histone acetylation [8962081]. | Yeast | — | — | — |
| YKU70 deletion | Deletion fo YKU70 shortens lifespan, but does not accelerate the normal aging process [10521401]. YKU70 null mutants are defective for non-homologous end-joining [8754818] and for telomeric silencing [9635192]. | Yeast | — | — | — |
| YKU80 deletion | Deletion of YKU80 shortens replicative lifespan, but does not accelerate the normal aging process [10521401]. YKU80 null mutant is defective for non-homologous end-joining [8754818] and for telomere silincing [9501103; 9635192] | Yeast | — | — | — |
| HSC82 deletion | Deletion of HSC82 has no effect on replicative lifespan, but shortens chronological lifespan [11361336]. | Yeast | — | — | — |
| LAC1 deletion | Deletion of LAC1 the homolog of LAG1 [9872981] has no effect on replicative lifespan in strain W303R and PSY316 [N. Bishop, G. Liszt, and L. Guarente, unpublished]. LAC1 null mutant has no obvious growth defect [10198056], but is synthetically lethal with LAG1 mutation [9872981]. | Yeast | — | — | — |
| MSN2 MSN4 double mutation | Deletion of MSN2 and MSN4 extends replicative lifespan and is further extended by cyr1::mTn [14741356]. Deletion of MSN2 and MSN4 does not significantly decrease chronological lifespan under AL, but attenuates chronological lifespan extension by water starvation and 0.5% glucose restriction [18225956] as well as cancels out lifespan extension of cyr1::mTn [14741356] and decreases chronological lifespan extension of ras2 deletion mutant [12586694]. Simulatnous deletion of MSN2 and MSN4 has no effect on chronological lifespan, but prevents lifespan extension by RAS2 deletion [12586694]. msn2 msn4 has no effect on replicative lifespan in PSY316, and does not prevent lifespan extension by DR [11000115] or by high osmolarity [12391171]. | Yeast | — | — | — |
| RAD1 mutation | Deletion of RAD1 has no effect on replicative lifespan [10207108]. | Yeast | — | — | — |
| RAD7 deletion | Deletion of RAD7 has no effect on replicative lifespan in PSY316 [10207108].
Mutation in RAD7 results in decrease repair of the non-transcribed strand in rDNA [8604332]. | Yeast | — | — | — |
| RSR1 deletion | Deletion of RSR1 (alias BUD1) shortens replicative lifespan [9789734]. | Yeast | — | — | — |
| RTG2 deletion | RTG2 is required for replicative lifespan extension associated with the retrograde response, a pathway that signals the functional status of mitochondria to the nucleus to regulate the expression of several genes [11024000]. RTG2 is not required for replicative lifespan extension by DR [11024000].
RTG2 null mutants are not petite [8422683], but display various nutrient auxotrphies and alterations of carbohydrate metabolism [7727418]. | Yeast | — | — | — |
| SIM1 deletion | Disruption of SIM1 shortens mean (87.5%), but not maximum, lifespan without causing any other gross changes in cell cycle parameter or growth characteristics [8810036].
Cells bearing deletions in CLB1-4 are unable to undergo mitosis and normally arrest in G2. SIM1 disruption in clb1-4 mutant backgrounds will allow a second round of DNA synthesis without mitosis [8574583]. sim1delta;uth1delta double mutants exhibit various defects, including binucleated cells, benomyl sensitivity, heat shock sensitivity, inability to store glycogen, sensitivity to starvation and failure of spores to germinate [10612745]. | Yeast | — | — | — |
| SLT2 deletion | Deletion of SLT2 has no effect on replicative lifespan in W303 strain [12640455].
SLT2 deletion increases rDNA silencing and rDNA recombination and decreases silencing at the telomeres and HM loci [Ray et al., 2003] as well as results in decreased phosphorylation of Sir3 [12640455]. | Yeast | — | — | — |
| ACH1 deletion | ACH1 deletion cells accumulate a high amount of extracellular acetic acid and display a reduced mean and maximum chronological lifespan. Maximum lifespan is reduced by 32%. Lifespan shortening is completely abrogated by alleviating the acid stress either by a DR regimen that prevents acetic acid production or by transferring chronologically aging mutant cells to water. Deletion of ACH1 is accompanied by reactive oxygen species accumulation, severe mitochondrial damage, and an early insurgence of apoptosis [22754872]. | Yeast | — | — | -32 |
| YKL069W deletion | Deletion of YKL069W increases sensitivity to oxidative stress and decreases replicative lifespan [19049972]. | Yeast | — | — | — |
| HST1 deletion | Deletion of HST1 blocks the residual replicative lifespan extension by hxk2 mutant in a sir2;fob1;hst2 triple mutant background [16051752]. However, DR can increases the replicative lifespan to a similar extent in sir2;fob1;hst1;hst2 quadruple mutant cells as in sir2;fob1 double mutant cells under 0.5, 0.05 and 0.005% glucose conditions and even by hxk2 deletion mutant [16741098; 17129213]. | Yeast | — | — | — |
| PCK1 deletion | Loss of Pck1 activity blocks chronological lifespan extension caused by water starvation. Knockout of PCK1 dramatically reduces chronological lifespan in both water (extreme DR) and glucose-containing medium. Deletion of SIR2 does not alter the lifespan of PCK1 deletion mutant, pck1-K514R, and pck1-K514Q mutants [19303850]. | Yeast | — | — | — |
| GSH1 deletion | Deletion of GSH1 confers deficiency in glutathione biosynthesis and further increases chronological lifespan under 0.5% glucose restriction, but does not extend chronological lifespan under 2% glucose [18840459]. Therefore, GSH1 has a positive interaction with DR [18840459]. | Yeast | — | — | — |
| ERG3 deletion | Deletion of ERG3 decreases replicative lifespan under AL, cancels out replicative lifespan extension of 0.5% glucose DR and results under DR also into a shorter replicative lifespan than under AL [18690010]. | Yeast | — | — | — |
| SUR4 deletion | Deletion of SUR4 cancels out replicative lifespan extension of 0.5% glucose DR [18690010]. | Yeast | — | — | — |
| LCB4 deletion | Deletion of LCB4 increases replicative lifespan and cancels out replicative lifespan extension of 0.5% glucose DR [18690010]. | Yeast | — | — | — |
| NFU1 deletion | NFU1 mutation slightly shortens the chronological lifespan under AL and the chronological lifespan of NFU1 mutants is not extended by 0.5% glucose DR [20421943]. | Yeast | — | — | — |
| FET3 deletion | FET3 mutation slightly shortens chronological lifespan under AL. Its chronological lifespan is not extended by 0.5% glucose or amino-acid DR [20421943]. FET3 is one of several iron related genes that are up-regulated in response to increasing strength of glucose DR [18679056]. | Yeast | — | — | — |
GenAge
GenDR
