Interventions

  • name effect species mean median maximum
    SIM1 deletion Disruption of SIM1 shortens mean (87.5%), but not maximum, lifespan without causing any other gross changes in cell cycle parameter or growth characteristics [8810036]. Cells bearing deletions in CLB1-4 are unable to undergo mitosis and normally arrest in G2. SIM1 disruption in clb1-4 mutant backgrounds will allow a second round of DNA synthesis without mitosis [8574583]. sim1delta;uth1delta double mutants exhibit various defects, including binucleated cells, benomyl sensitivity, heat shock sensitivity, inability to store glycogen, sensitivity to starvation and failure of spores to germinate [10612745]. Yeast
    TCO89 deletion TCO89 deletion increases chronological lifespan, increases mitochondrial oxygen consumption, but decreases mitochondrial and cellular ROS in early stationary phase [21641548]. Deletion of TCO89 cancels out replicative lifespan extension by moderate DR [18690010]. Yeast
    TSA1 deletion Disruption of TSA1 shortens chronological lifespan [15129730]. Replicative lifespan extension by DR in sir2;fob1 double mutant is reduced by TSA1 deletion mutant. Wild-type cells require TSA1 to fully extend lifespan. Mutation in CDC35 (adenylate cyclase), a genetic mimetic of DR, is dependent on TSA1 to extend lifespan [21884982]. Yeast
    HPR1 deletion Deletion of HPR1 decreases replicative lifespan [11756539] Yeast
    VAM7 deletion VAM7 deletion decreases replicative lifespan under AL and blocked DR-mediated lifespan extension. Replicative lifespan decreases by 70% on DR in VAM7 deletion mutant [18690010]. Yeast
    NYV1 deletion Deletion of NYV1 cancels out replicative lifespan extension of 0.5% glucose restriction and results under DR also into a shorter replicative lifespan than under AL [18690010; 22622083]. Thus, NYV1 deletion blocks DR-lifespan prolongation [18690010]. Yeast
    SLT2 deletion Deletion of SLT2 has no effect on replicative lifespan in W303 strain [12640455]. SLT2 deletion increases rDNA silencing and rDNA recombination and decreases silencing at the telomeres and HM loci [Ray et al., 2003] as well as results in decreased phosphorylation of Sir3 [12640455]. Yeast
    LAT1 deletion Deleting LAT1 abolishes replicative lifespan extension induced by 0.5% and 0.05% glucose restriction [17200108]. Yeast
    CIT2 deletion Deletion of CIT2 has no effect on replicative lifespan [10224252]. Yeast
    ERG6 deletion Deletion of ERG6 cancels out replicative lifespan extension of 0.5% glucose DR and results under DR also into a shorter replicative lifespan than under AL [18690010]. Yeast
    COQ3 deletion Deletion of COQ3 decreases chronological lifespan and renders cells respiratory deficient and sensitive to hydrogen peroxide [12586694]. Yeast
    ATG17 deletion ATG17 deletion decreases replicative lifespan under AL and blocks DR-lifespan extension. ATG17 mutant's replicative lifespan decreases by 70% on DR [18690010]. Yeast
    CPR7 deletion Deletion of CPR7 has no effect on lifespan replicative lifespan, but increases chronological lifespan [11361336] Yeast
    ATG15 deletion Deletion of ATG15 does not affect the lifespan significantly on AL, while DR shortens replicative lifespan of ATG15 deletion mutant by 28% [18690010]. Yeast
    CTF4 deletion Deletion of CTF4 results in an approximately 75% reduced mean replicative lifespan [12024027]. Yeast
    CYT1 deletion Deletion of CYT1 increases replicative lifespan by 15% in the alpha strain and decreases replicative lifespan by 20% in a strain. Deletion of CYT1 decreases replicative lifespan and cancels out replicative lifespan extension by HAP4 overexpression. Initially, it was shown that deletion of CYT1 also prevents lifespan extension by 0.5% glucose restriction [12124627], but later it was shown that either 0.5 or 0.05 % glucose restriction increases replicative lifespan of cyt1Delta cells [16311627]. Yeast
    STE12 deletion Deletion of STE12 has no effect on replicative lifespan in strain PSY142 or BKY1-14c [7859289]. STE12 null mutants are sterile [6993497]. Yeast
    VAC14 deletion VAC14 mutants have a single vacuole and shortened lifespan on normal media [16293764]. Yeast
    OSH4 deletion Deletion of OSH4 decreases mean replicative lifespan by 18% [Xia et al., unpublished]. Yeast
    OSH6 deletion OSH6 deletion does not affect lifespan under normal conditions, but it abrogates the lifespan extension by 0.5% glucose restriction [Xia et al. unpublished]. Yeast
    VPS21 deletion Lack of VPS21 reduces lifespan under starvation conditions to a level similiar to that of wild-type cells incubated in synthetic complete medium and therefore blocked the lifespan-extending effect of DR [20657825]. Yeast
    AVO2 deletion Deletion of AVO2 extends chronological lifespan [21641548]. Yeast
    AGP1 deletion Deletion of AGP1 extends chronological lifespan [16418483]. Yeast
    OSH2 deletion Deletion of OSH2 decreases mean chronological lifespan [20657825]. Yeast
    FOB1 deletion Mutation in FOB1 extends replicative lifespan by 30-50% [10230397]. FOB1 mutation increases replicative lifespan by 25% in the alpha strain and by 10% in a strain [19030232]. FOB1 mutant exhibit an about 20% mean replicative lifespan increase [15722108]. Deletion of FOB1 causes extension in the short life span of the sir2 mutant by around 50% [10521401]. Mutation of the FOB1 gene slows the generation of rDNA circles and thus extends life span by approximately 30% in W303 and 50% in K2307 [10230397]. Even in cells lacking both Sir2 and Fob1, nicotinamide prevents the lifespan extension by DR [16311627]. Yeast
    Interventions are an extension of GenAge and GenDR.