| p53 mutation | Globally lack of p53 decreases lifespan [16303568]. Loss of p53 activity slightly shortens the lifespan. Mutants that lack p53 survive well up to 50 days, but mortality rate increases relative to wild-type at later ages. p53 mutant animals are extremely sensitive to irradiation [12935877]. | Fly | — | — | — |
| Pi3K92E mutation | Heterozyogous mutation in Pi3K92E fails to extend lifespan [11292874] and it is recessive lethal. | Fly | — | — | — |
| Scgdelta deletion | Deletion of Scgdelta has detrimental effects on the flight muscles of adult animals and heart function. Median lifespan is reduced 15-30% [17855453]. | Fly | — | -15 to -30 | — |
| rut mutation | Two rutabaga mutants, rut1 and rut2080, have significantly shortened lifespans [17369827]. | Fly | — | — | — |
| mld heterozygous mutation | Female, but not male, heterozygous mutants display a 42% increase in mean lifespan at 29 degrees Celsius. DTS-3 +/- female adults exhibit a 50% reduced ecdysone titer and reduced fertility [12610309]. Female, but not male, heterozygoutes also exhibit a temperature-dependent increase in starvation resistance. | Fly | +42 | — | — |
| DLP mutation | DLP mutants have a 20% shorter mean lifespan and reduced female fertility [17933869]. | Fly | -20 | — | — |
| EcR mutation | Mutant heterozygotes in EcR live on mean 40%-50% longer than controls [12610309; reviewed in 12610294]. Homozygous mutants in EcR are inviable. The developmental time and weight of EcR+/- mutants is the same as control, but resistance to temperature, oxidative stress, and starvation is increased in heterozygotes [12610309]. | Fly | +40 to +50 | — | — |
| l(3)DTS3 mutation | Female, but not male, heterozygous mutants exhibit a 42% increase in mean lifespan [12610309]. | Fly | +42 | — | — |
| egm mutation | Mutation in egm confers resistance to oxidative stress and extends the lifespan [16434470]. | Fly | — | — | — |
| GLaz mutation | Loss-of-function mutation of GLaz which decreases its expression of GLaz results in shorter lifespan and decreased resistance to oxidative stress in males [16581513]. | Fly | — | — | — |
| hypomoprhic hep mutation | A hypomorphic allele of hep (hep1) laerlgy prevents lifespan extension caused by puc heterozygosity [14602080]. | Fly | — | — | — |
| Dcr-2 mutation | Median lifespan of homozyogous and transheterozyogous Dcr-2 mutants is reduced by 18-36% in males and by 27-36% in females. Dcr-2 loss changes the expression of mostly metabolic genes implicated in stress resistance and aging. Dcr-2 mutants are hypersensitive to oxidative, endoplasmic reticulum, starvation and cold stress as well as abnormal lipid and carbohydrate metabolism [21889502].
| Fly | -18 to -36 | — | — |
| InRE19/InRp5545 transheterozygous mutation | Mutations in InR (InRE19/InRp5545 transheterozygous) result in dwarf females with extended lifespan of up to 85% and dwarf males with reduced late age-specific mortality (although no significant change in lifespan) [11292875]. | Fly | +85 | — | — |
| InrGC25/InrE19 transheterozygous mutation | InrGC25/InrE19 transheterozygous animals are short-lived an exhibit an elevated rate of age-independent mortality [11292874]. | Fly | — | — | — |
| Ilp2/Ilp3/Ilp5 mutation | Ilp5 null mutants have a normal lifespan under AL and a normal DR response. Ilp2 Ilp3 Ilp5 triple null mutants fail to have a normal response to DR. Their response is right shifted, with mutants being shorter-lived compared to wild-type on low but longer-lived on high yeast concentrations [20195512]. | Fly | — | — | — |
| Ilp2 mutation | Ilp2 null mutants are significant longer-lived with a 8-13% longer median lifespan [20195512]. | Fly | — | +8 to +13 | — |
| Thor mutation | Null mutation in Thor (alias d4E-BP) causes a significant decrease in longevity (-25% median lifespan in males). foxo (alias dFOXO) and Thor null mutants are compromised in stress resistant. Stress resistance of foxo null mutants is rescued by Thor overexpression [16055649]. Thor null mutants cancel out DR-induced lifespan extension, because mutants exhibit a diminished change in lifespan when nutrient conditions were varied. Thor null mutants have a wild-type similar reduction in egg production upon DR [19804760]. | Fly | — | -25 | — |
| foxo mutation | foxo null mutants are highly and significantly shorter-lived than wild-type on all food dilutions apart from 0.1 SY and under starvation. foxo null mutants are not more sensitive to starvation than wild-type [18241326]. | Fly | — | — | — |
| Sir2 mutation | A decrease in Sir2 (alias dSir2) blocks the life-extending effect of caloric reduction or rpd3 mutations [15520384]. Sir2 mutation does not reduce lifespan under AL [15520384]. | Fly | — | — | — |
| Dominant negative Tor | Expression of a dominant-negative form of Tor extends lifespan [15186745]. Ubiquitious overexpression of dTOR with the da-GAL4 driver of UAS-dTOR(FRB) which contains the 11kDA FKB12-rapamycin binding domain led to a mean and maximum lifespan increase of 15% (24%) and 29% at 29°C and of 50% (26%) and 13% at 25°C, respectively [15186745].
Overexpression of the dominant-negative form of Tor specifically in the fat and muscle tissues is sufficient to extend the mean and maximum lifespan by 24 and 19%, respectively [15186745].
Overexpression of UAS-dTOR(WT) or UAS-dTOR(TED) prevents eclosion to adulthood [15186745]. | Fly | +15 to +50 | +13 to +29 | — |
| Dominant-negative S6k | Ubiquitous overexpression of a dominant-negative form of S6k (alias dS6K) increases mean lifespan by 22%. Overexpression of a dominant-negative form of S6k protects mutants from deleterious effects of rich food, as if mimicking the effect of DR [15186745]. | Fly | +22 | — | — |
| Constitutive active S6k overexpression | Overexpression of a constitutively active form of S6k (alias dS6K) decreases mean lifespan by 34% at 29°C [15186745]. | Fly | -34 | — | — |
| Mnt Mutation | A dMnt null allele results in flies with larger cells, increased weight, and decreased lifespan [16055719]. | Fly | — | — | — |
| mys Mutation | mys mutants exhibit ameliorated age-related declines in locomotor activity and an increase in mean lifespan of 20% [14570233]. | Fly | +20 | — | — |
| Heterozygous hypomorphic Rpd3 mutation | Males heterozygous for hypomorphic (partial loss-of-function) mutation of Rpd3 have a lifespan extension of 33%. Females heterozygous for a hypomorphic allele have a 52% increase in lifespan. Longevity increases to the same extent in wild-type under low-calorie diet and rpd3 mutants fed normal diet [12459580]. | Fly | +33 to 52 | — | — |
GenAge
GenDR
