| p53 overexpression | Overexpression of wild-type Dmp53 during adult life has no significant effect on lifespan [16303568]. | Fly | — | — | — |
| Hsp70 locus overexpression | Overexpression of the Hsp70 locus (containing Hsp70Bb and Hsp70Bc) in transgenic flies extends lifespan as much as 7.9% [9363888]. | Fly | +7.9 | — | — |
| Atg8a overexpression | Enhanced expression of Atg8a in older fly brains extends average adult lifespan by 56% and promotes resistance to oxidative stress [18059160]. | Fly | +56 | — | — |
| Lazarillo supplementation | Extracellular forms of Laz have autocrine and paracrine protecting effects for oxidative stress-challanged Drosophila S2 cells. Local effects of GPI-linked Laz inside and outside the nervous system promote survival upon different stress forms, and extend lifespan and healthspan of the flies in a cell-type dependent manner. Ectopic enhancement of Laz expression increases mean, median, and maximum lifespan. Laz overexpression (via the use of a ubiquitous da-GAL4 driver) increases median lifepan by 28.3% (p < 0.0005). Overexpression of Laz specifically in muscles and brain (via GAL4109(2)80 driver) increases median lifespan by 43.5%. Laz overxpression in dopaminergic and serotenergic neurons and epidermis increases median lifespan bt 31.4% (p < 0.0005) [22846641]. | Fly | — | +28.3 to +43.5 | — |
| Sin3A overexpression | Overexpression of Sin3A increases mean and maximum lifespan by 13% and 3%, respectively [22366109]. | Fly | +13 | — | +3 |
| pex1 mutation | Mutation of the promoter region of pex1(S4868), results in lower expression levels and increases lifespan in males and females by 16% and 13%, respectively. pex1 mutation increases mean and maximum lifespan and males/females by 27-23/14-19% and 0-15%, respectively [22509016]. | Fly | +14 to +27 | — | 0 to +15 |
| CG30427 overexpression | Overexpression of CG30427 in males increases mean lifespan by 18% [22366109]. | Fly | +18 | — | — |
| rpr overexpression | Flies with ablated wings caused by overexpressing reaper (UAS-rpr) with a wing-specific Gal4 enhancer trap (1096-Gal4) exhibit only a 14% extension in lifespan compared to controls which exhibit a 61% extension upon DR [22768842]. | Fly | — | — | — |
| to overexpression | Overexpression of to adult neurons, pericerbral or abdonimal fat body increases male and female lifespan. to overexpression in the adult nervous system, head fat body and abdominal fat body results in 25, 20 and 12-18% increase of mean lifespan. on average the mean lifesapn is extended for males and females by 18 and 26%, while maximum lifespan of male and female is increased by 13 and 25% [20519778]. | Fly | +12 to +26 | — | +13 to +25 |
| PGRP-LF overexpression | Overexpression of PGRP-LF increases mean and maximum lifespan by 13% and 24% [22366109]. | Fly | +13 | — | +24 |
| ImpL2 overexpression | Lmp-L2 over-expression, ubiquitous or restricted to DILP-producing cells and/or gut and fat body, extends lifespan even if induced at adult onset [21108726]. Overexpression of ImpL2 increases mean and maximum lifespan by 15% and 23%, respectively. Lifespan is reduced when Impl2 is strongly over-expressed throughout the adult by the conditional GS driver, act-GS-GAL4 or da-GS-Gal3, while restricted over-expression of the ImpL2 in fat cells by using S106-GS-Gal4, which increases mRNA level about 6-fold extends lifespan in both sexes [22366109]. mRNA for Impl2 was strongly elevated in sterile, long-lived flies [18434551]. | Fly | +15 | — | +23 |
| Hsp23 overexpression | Overexpression of Hsp23 increases mean lifespan by more than 30% and increases the premortality phase [14734639]. | Fly | +30 | — | — |
| CG10383 overexpression | Overexpression of CG10383 in males increases mean and maximum lifespan by 12% and 8%, respectively [22366109]. | Fly | +12 | — | +8 |
| Akh overexpression | Overexpression of Akh in a ubiquitousness manner enhances fat metabolism (significant increase in triglyceride synthesis and breakdown under AL), spontaneous activity (148% on AL and 154% on DR), and lifespan on AL (33%). However, despite and increase in movement under DR, lifespan is not increased under a restricted diet [22768842]. | Fly | — | +33 | — |
| SIFR overexpression | Overexpression of SIFR in males extends mean and maximum lifespan by 23% and 3%, respectively [22366109]. | Fly | +23 | — | +3 |
| NF1 overexpression | Overexpression of NF1 increases mitochondrial respiration and reduced ROS production. It increases mean lifespan by 49% in males and 68% in females and maximum lifespan by 38% in males and 52% in females. It also improved reproductive fitness [17369827]. | Fly | +49 to +68 | — | +38 to +52 |
| sm overexpression | Overexpression of sm in males increases mean and maximum lifespan by 29% and and 16%, respectively [22366109]. | Fly | — | — | — |
| CG10916 overexpression | Overexpression of CG10916 in males increases mean and maximum lifespan by 27% and 26%, respectively [22366109]. | Fly | +27 | — | +26 |
| Cat overexpression | Simultaneous overexpression of catalase and Sod1 results in a one-third (i.e. 30%) lifespan extension, a slower rate of mortality acceleration, and a delayed loss in physical performance, but neither has any effect on lifespan alone [8108730]. Targeted overexpression of CAT in the mitochondrial matrix has no effect on lifespan at 25 degree Celsius, slightly shortens lifespan at 29 degree Celsius as is associated with increased resistance to endogenous hydrogen peroxide, paraquat, and cold stress [12521602]. | Fly | — | — | — |
| Cct1 overexpression | Overexpression of Cct1 from a doxycycline-inducible promoter results in a 6 - 8% increase in mean lifespan (in the PdL x rtTA; Oregon-R x rtTA strain) [12620118]. | Fly | +6 to +8 | — | — |
| cher overexpression | Overexpression of cher from a doxycycline-inducible promoter results in a 7 - 9% increase in mean lifespan (in PdL x rtTA; Oregon-R x rtTA) [12620118]. | Fly | +7 to +9 | — | — |
| CG13890 overexpression | Overexpression of CG13890 (DCI) throughout the whole body increases mean and median lifespan by 35 and 31%, but decreases maximum lifespan by 6%, increases stress resistant (to paraquat and starvation), consistently reduces the mortality rate across adult ages and reduces the lifespan extension of DR by 15% [22997544].
CG6783 overexpression increases the dFOXO nuclear localization in the fat-body. mRNA levels of dFOXO target genes l(2)efl and 4E-BP in the adult whole bodies increases in response to overexpression of CG6783 [22997544]. | Fly | +35 | +31 | +6 |
| fabp overexpression | Overexpression of fabp (CG6783) throughout the whole body increases mean, median and maximum lifespan by 77, 81 and 13%, increases stress resistant (to paraquat but not starvation), consistently reduces mortality rate across adult ages and reduces the lifespan extension of DR by 12% [22997544].
fabp overexpression increases the dFOXO nuclear localization in the fat-body. mRNA levels of dFOXO target genes l(2)efl and 4E-BP in the adult whole bodies increases in response to overexpression of fabp [22997544].
Females of the genotype Act-GS-Gal4 > UAS-CG6783 exhibit an increase in median lifespan compared to uninduced control in response to feeding with RU486-containing food from day 3 of adulthood (P < 0.0001). Mean lifespan is extended by 10, while maximum lifespan is decreased by 11% [22997544]. | Fly | +77 | +81 | +13 |
| gig overexpression | Overexpression of gig, also known as dTsc2, results in lifespan extension. Overexpression of dTsc2 increases mean lifespan by 20% and 12%, at 25°C and 29°C, respectively, and protects from deleterious effects of rich food, as if mimicking the effect of DR [15186745].
Overexpression of dTsc2 via a UAS promoter in the eye using the driver gmr-GAL4 or in the nervous system by using appI-GAL4 does not extend the lifespan. Using the drivers 24BGAL4 and PO188-GAL4, enhancer traps that are predominantly expressed in the muscle and fat results in mean lifespan extension of 27% and 37%, respectively, at 29°C [15186745]. Fat-specific drivers DJ634-GAL4 and PO163-GAL4 when used to overexpress dTsc2, also led to a mean lifespan extension of 22% and 31%, respectively, at 29°C [15186745]. | Fly | +20 to +31 | — | — |
| Constitutive active S6k overexpression | Overexpression of a constitutively active form of S6k (alias dS6K) decreases mean lifespan by 34% at 29°C [15186745]. | Fly | -34 | — | — |
GenAge
GenDR
