| nhr-62 Overexpression | Wild-type (N2) worms with extrachromosomal array dhEx627 (carrying a wild-type nhr-62) exhibit a significant increase in lifespan compared to wild-type (p<0.001) [Heestand et al. 2013]. | Worm | — | — | — |
| mir-71 overexpression | Gain-of-function of mir-71 increases lifespan [21129974]. Extra copies of mir-71 extend the lifespan with an increase in lifespan by 15 - 25% [22482727], | Worm | +15 to +25 | — | — |
| mir-246 overexpression | mir-246 overexpression increases mean and maximum lifespan by 6 and 5 - 14%, respectively [21129974]. | Worm | +6.3 | — | +4.8 to +14.3 |
| mir-239 overexpression | Overexpressing mir-239 decreases mean and maximum lifespan by 13 and 17 - 33%, respectively [21129974]. | Worm | -13 | — | -17 to -33 |
| lyz overexpression | Overexpression of lyz increases mean, median, and maximum lifespan by 26, 30 and 44% [22737090]. | Worm | +26 | +30 | +44 |
| lys-1 overexpression | Overexpression of lys-1 increases mean and maximum lifespan by 5 and 26%, but has no significant effect on median lifespan [22737090]. | Worm | +5 | — | +26 |
| lmp-2 overexpression | Overexpression of lmp-2 increases mean, median and, maximum lifespan by 32, 35, and 41% [22737090]. | Worm | +32 | +35 | +41 |
| lin-4 overexpression | Overexpressing lin-4 extends lifespan [16373574]. | Worm | — | — | — |
| lin-14 gain-of-function mutation | A gain-of-function mutation in lin-14 decreases lifespan [16373574]. | Worm | — | — | — |
| lars-2 mutation | A mutation that impairs mitochondrial function was associated with a longer lifespan. Mutation of lrs-2/lars-2(mg312) extends lifespan and is associated with impaired mitochondrial function. The recessive allele mg312 of lars-2 extends lifespan by 200% at 20 degree Celsius and 30% at 25 degree Celsius. Lifespan extension by mg312 was not dependent on daf-16(mgDf47). Homozygous lars-2(mg312) worms had multiple pleotropies like lower rates of growth, pumping and defecation as well as remain the size of early L4 worms and are sterile, with an arrested gonad that exhibited no germ-cell differentiation lars-2 is ubiquitously express, with prominent expression in body-wall muscle and neurons, with a mitochondrial subcellular localisation. Mitochondria of lars-2 are noticeably disorganized, swollen and sometimes fused. lars-2 animals have lower ATP content and oxygen consumption [12447374]. | Worm | +30 to +200 | — | — |
| jnk-1 overexpression | Overexpression of jnk-1 increases lifespan by 40% [15767565; 23097426].
JNK-1 overexpression extends the lifespan in a daf-16-dependent manner. JNK-1 overexpression does not extend the lifespan of animals unable to synthesize miRNAs, i.e. pash-1(mj100) [23097426]. | Worm | +40 | — | — |
| ins-1 overexpression | Increased dosage of ins-1 under its own promoter as well as a heat shock promoter increases lifespan by 25% and is also able to increase the lifespan of daf-2 mutants. Overexpression of ins-1 also causes an increase in dauer formation and can enhance the dauer formation of daf-2 mutants [11274053]. | Worm | +25 | — | — |
| INS overexpression | Expression of human insulin under an inducible heat shock promoter increases nematode lifespan by 25% and is also able to enhance the lifespan of daf-2 mutants [11274053]. | Worm | +25 | — | — |
| hsp-6 overexpression | Overexpression of hsp-6 from a muscle-specific promoter extends lifespan mean and maximum lifespan by 43 and 45% relatively to animals expressing GFP from the same promoter [11959102]. | Worm | +43 | — | +45 |
| hsp-16 overexpression | Overexpression of the hsp-16 loci enhances stress resistance and extends mean lifespan by 11% at 20 degree Celsius. Lifespan extension by hsp-16 overexpression requires daf-16 [12882326]. | Worm | +11 | — | — |
| hsf-1 overexpression | Overexpression of hsf-1 extends the mean, median, and maximum lifespan by 37, 35, and 29% [22737090]. | Worm | +37 | +35 | +29 |
| faah-1 overexpression | faah-1 overexpression reduces eicosapentaenoyl ethanolamide (EPEA), palmitoleyol ethanolamide, linoleyol ethanolamide, as well as arachidonoyl ethanolamide (AEA) levels, delays development, increases thermal stress resistance, and was associated with mean and maximum adult lifespan extension by 19 and 35%, respectively, in presence of abundant food but not under (two different protocols of) DR. Overexpression in pharynx was largely sufficient for this lifespan extension [21562563]. | Worm | +19 | — | +35 |
| daf-18 overexpression | Overexpression increases adult lifespan in individual tissues [16153634]. | Worm | — | — | — |
| daf-16 overexpression | Overexpression of wild-type DAF-16 modestly increases lifespan by 20% [11747825]. | Worm | +20 | — | — |
| cup-4 overexpression | cup-4 overexpession reduces oxidative stress resistance and shortens lifespan of wild-type under AL [19783783]. | Worm | — | — | — |
| cst-1 overexpression | Overexpression of cst-1 extends lifespan and delays aging in a daf-16-dependent manner [16751106]. | Worm | — | — | — |
| Coq7 overexpression | Transgenic overexpression of mouse Coq7 reverts the extended lifespan of clk-1 mutants [11511092]. | Worm | — | — | — |
| clk-1 overexpression | Overexpression of clk-1 shortens lifespan and is associated with increased mitochondrial activity [10202142]. | Worm | — | — | — |
| clk-1 overexpression | Overexpression of clk-1 shortens lifespan and is associated with increased mitochondrial activity [10202142]. | Worm | — | — | — |
| cbp-1 overxpression | Overexpression of cbp-1 does not significantly affect lifespan [19924292]. | Worm | — | — | — |
GenAge
GenDR
