| RAD27 deletion | Deletion of RAD27 results in signs of premature aging and approximately 60% reduction in mean replicative lifespan [12024027]. | Yeast | -60 | — | — |
| SUR4 deletion | Deletion of SUR4 cancels out replicative lifespan extension of 0.5% glucose DR [18690010]. | Yeast | — | — | — |
| RAS2 deletion | RAS2 deletion causes a 23% decrease in mean and a 30% decrease in maximum lifespan [8034612]. Deletion of RAS2 leads to a longer chronological lifespan [21076178]. Deletion of the RAS2 gene, which functions upstream of CYR1, doubles the mean chronological lifespan by a mechanism that requires Msn2/4 and Sod2 [12586694]. DR further extends chronological lifespan of ras2Delta [18225956]. | Yeast | -23 | — | -30 |
| LCB4 deletion | Deletion of LCB4 increases replicative lifespan and cancels out replicative lifespan extension of 0.5% glucose DR [18690010]. | Yeast | — | — | — |
| RAD52 deletion | Deletion in RAD52 causes a 75% reduction in mean replicative lifespan in PSY316 strain [10207108]. Similiar reduction of lifespan occurs in strains W3031-A and W303R [M. Baeberlein, M. McVey, and L. Guarente, unpublished].
RAD51 is required for formation of extrachromosomal rDNA circles [10207108], but not for replication fork pasuing nor DNA breakage with the rDNA [10693764]. | Yeast | -75 | — | — |
| RAD7 deletion | Deletion of RAD7 has no effect on replicative lifespan in PSY316 [10207108].
Mutation in RAD7 results in decrease repair of the non-transcribed strand in rDNA [8604332]. | Yeast | — | — | — |
| NFU1 deletion | NFU1 mutation slightly shortens the chronological lifespan under AL and the chronological lifespan of NFU1 mutants is not extended by 0.5% glucose DR [20421943]. | Yeast | — | — | — |
| RAD9 deletion | Deletion results in mutants with a 2-fold decrease in mean and maximum chronological lifespan under conditions of nutrient depletion [17710147]. Mutation of RAD9 shortens lifespan by 30% in DBY747 [7806576] and in strain W303 [11290710].
RAD9 mutation has no effect on telomeric silencing or length [10924458]. | Yeast | -30 | — | — |
| FET3 deletion | FET3 mutation slightly shortens chronological lifespan under AL. Its chronological lifespan is not extended by 0.5% glucose or amino-acid DR [20421943]. FET3 is one of several iron related genes that are up-regulated in response to increasing strength of glucose DR [18679056]. | Yeast | — | — | — |
| PDE2 Deletion | Deletion of PDE2 decreases mean replicative lifespan by 26% [11000115] and also results in a shorter chronological lifespan [21076178].
PDE2 null mutant are sensitive to oxidative stress [10394911]. | Yeast | -26 | — | — |
| RAS1 deletion | Deletion in RAS1 increases mean (23%) and maximum (29%) replicative lifespan (in SP1) [8034612]. RAS1 deletion increases replicative lifespan by 15% in the alpha strain [19030232]. However, deletion of RAS1 slightly shortens chronological lifespan (in SP1) [12586694]. | Yeast | +15 to +23 | — | +29 |
| ATG16 deletion | Under AL atg16 mutation shortens chronological, but not replicative lifespan. 0.5% glucose DR extends chronological lifespan of atg16 mutants, but amino-acid DR does not extend the short chronological lifespan of atg16 mutants (similar to several other autophagy mutants). ADE4 deletion in atg16 mutants results only in a partial extension of chronological lifespan by 0.5% glucose DR. The long chronological lifespan of tor1 mutants requires ATG16 [20421943]. | Yeast | — | — | — |
| ATG2 deletion | ATG2 deletion prevents chronological lifespan extension induced by amino-acid DR [20421943]. | Yeast | — | — | — |
| RIM15 deletion | RIM15 deletion results in 50% reduction of maximal chronological lifespan [11292860] and consistently decreases chronological lifespan under AL [21076178]. Rim15 is required for chronological lifespan extension caused by deficiency in RAS2, TOR1, or SCH9, as well as by 0.5% glucose restriction, but not by water starvation [18225956]. | Yeast | — | — | — |
| VPS30 deletion | VPS30 deletion prevents chronological lifespan extension induced by amino-acid DR [20421943]. | Yeast | — | — | — |
| HXT17 deletion | HXT17 mutation extends both replicative and chronological lifespan as well as cancels out DR-induced replicative and chronological lifespan extension. Mean and maximum replicative lifespan are extended by 27 and 49%, respectively [21584246]. | Yeast | +27 | — | +49 |
| SOD2 deletion | SOD2 deletion decreases replicative lifespan by 72% [17460215]. SOD2 deletion decreases chronological lifespan [21076178]. Deletion of SOD2 decreases chronological lifespan in wild-type and abolishes chronological lifespan extension in sch9Delta mutants as well as decreases chronological lifespan in cyr1:mTn mutants [12586694].
SOD2 deletion mutants are hypersensitive to oxygen and grow poorly in ethanol [10222047]. | Yeast | -72 | — | — |
| REI deletion | REI1 deletion increases mean replicative lifespan by about 40% [16293764] in the alpha and a strains [19030232]. | Yeast | +40 | — | — |
| GUP1 deletion | GUP1 deletion extends mean and maximum replicative lifespan by 32 and 30%, respectively, as well as chronological lifespan. DR-induced maximal replicative lifespan extension is not further increased by GUP1 deletion, while gup1 mutant displayed longer chronological lifespan under DR [21584246]. | Yeast | +32 | — | +30 |
| AIM4 deletion | AIM4 (alias SOY1) deletion increases chronological and replication lifespan, which is non-additive with DR. On AL mean and maximum replicative lifespan are extended by 63 and 69%, respectively. DR appears to decrease aim4-induced replication lifespan extension, indicating a negative interaction. aim4 mutation does not change DR-induced chronological lifespan extension [21584246]. | Yeast | +63 | — | +69 |
| HHF1 deletion | HHF1 deletion extends mean and maximum replicative by 45 and 69%, respectively, as well as chronological lifespan. Chronological lifespan extension by HHF1 deletion and DR is non-synergistic. DR appears to extend replicative lifespan more when combined with hhf1 mutation, whereas DR does not change hhf1-induced replicative lifespan extension, suggesting a positive interaction [21584246]. | Yeast | — | — | — |
| ROM2 deletion | Deletion of ROM2 increases mean replicative lifespan of the alpha strain by about 50% [16293764]. ROM deletion mutant replicative lifespan increases by 49% in the alpha strain and 16% in a strain. Deletion of ROM2 increases replicative lifespan by 38% in the alpha strain and by 29.3% in the a strain (34.2% in both) [19030232]. | Yeast | +16 to +50 | — | — |
| SFA1 deletion | Deletion of SFA1 alone has no effect on replicative lifespan. sfa1;yhb1 double mutant cancels out the ability of moderate DR to extend replicative lifespan, but not chronological lifespan. Yhb1 and Sfa1 may play redundant roles hb1 and Sfa1 may play redundant roles [21584246]. | Yeast | — | — | — |
| RPD3 deletion | Deletion of the histone deacetylase gene RPD3 extends lifespan by 41%, independently of an intact Sir silencing complex (in the short lived YSK661 strain) [10512855]. Deletion of RPD3 extends replicative lifespan and there was no additive effect by neither 0.1% glucose nor amino acid restriction [12213553].
RPD3 deletion increases rDNA silencing in a partially SIR2-dependent manner [10082585].
Its effects on chromatin functional state were evidenced by enhanced silencing at the three known heterochromatic regions in the genome, the silent mating type (HM), subtelomeric, and rDNA loci, which occurred even in the absence of SIR3 [10512855]. | Yeast | +41 | — | — |
| SIR3 deletion | Deletion of SIR3 shortens replicative lifespan by approximately 20% [10521401]. SIR3 mutants exhibit a loss of silencing at the silent mating loci [6098447; 3297920] and telomerease [1913809] and have a slighlty elevuated level of rDNA marker loss [10521401]. The lifespan reduction of SIR3 deletion is suppressed by preventing mating type heterozygosity and is therefore probably due to the simultaneous expression of a and alpha mating-type information, which indirectly causes an increase in rDNA recombination and likely increases the production of extrachromosomal rDNA circles [10521401]. Deletion of SIR3 itself has little effect on lifespan, although it markedly accelerates the increase in cell generation time that occurs during aging [10512855]. | Yeast | -30 | — | — |
GenAge
GenDR
