| ucp-4 overexpression | Overexpression reduces mean lifespan by 5%, has no significant effect on median lifespan, and slighlty increases maximum lifespan by 12% [22737090]. | Worm | -5 | — | +12 |
| mir-239 overexpression | Overexpressing mir-239 decreases mean and maximum lifespan by 13 and 17 - 33%, respectively [21129974]. | Worm | -13 | — | -17 to -33 |
| abu-11 overexpression | Overexpression of abu-11 extends mean lifespan by 9% to 28% [16256736]. | Worm | +9 to +28 | — | — |
| mir-246 overexpression | mir-246 overexpression increases mean and maximum lifespan by 6 and 5 - 14%, respectively [21129974]. | Worm | +6.3 | — | +4.8 to +14.3 |
| rps-27 RNAi | Knockdown of rps-27 by RNAi extends mean and maximum lifespan by 50 and 44%, respectively [19293945]. | Worm | +50 | — | +44 |
| sir-2.1 overexpression | sir-2.1 overexpression extends lifespan by about 50% and this lifespan extension depends on DAF-16 activity as it is suppressed by mutation in daf-16 and it does not synergize with daf-2 [11242085].
Overrexpression of sir-2.1 synergizes with TGF-beta mutation (daf-4 and daf-1) for dauer formation [11242085]. | Worm | +50 | — | — |
| Overexpression of constitutive nuclear SKN-1 | skn-1 transgenes that overexpress a constitutive nuclear form of SKN-1 in the intestine extend the mean lifespan by 5-21%, independently of DAF-16 [18358814]. | Worm | +5 to +21 | — | — |
| lys-1 overexpression | Overexpression of lys-1 increases mean and maximum lifespan by 5 and 26%, but has no significant effect on median lifespan [22737090]. | Worm | +5 | — | +26 |
| aakg-2 overexpression | Overexpression of aakg-2 extends mean, median, and maximum lifespan by 47, 45, and 35% [22737090]. | Worm | +47 | +45 | +35 |
| phi-44 RNAi | phi-44 RNAi leads to 46% mean and 50% maximum lifespan extension. Lifespan extension by phi-44 is not suppressed by daf-16.
phi-44 RNAi animals have lower ATP content and oxygene consumption [12447374]. | Worm | +46 | — | +50 |
| hsp-6 overexpression | Overexpression of hsp-6 from a muscle-specific promoter extends lifespan mean and maximum lifespan by 43 and 45% relatively to animals expressing GFP from the same promoter [11959102]. | Worm | +43 | — | +45 |
| ucp2 overexpression | Overexpression of ucp2 increases mean, median, and maximum lifespan by 42, 40, and 26%, which is non-additive with sDR [22737090]. | Worm | +42 | +40 | +26 |
| old-1 overexpression | Overexpression of old-1 in transgenic animals increases mean and maximum lifespan by 40-100% (average 65%) and 97%, respectively. old-1 overexpression of increases stress resistance (to heat by 20% and ultraviolet irradiation by 33%) without altering development or fertility. Effects of old-1 on lifespan and stress resistance is under regulation of daf-16 [9768365]. | Worm | +40 to +100 | — | +97 |
| jnk-1 overexpression | Overexpression of jnk-1 increases lifespan by 40% [15767565; 23097426].
JNK-1 overexpression extends the lifespan in a daf-16-dependent manner. JNK-1 overexpression does not extend the lifespan of animals unable to synthesize miRNAs, i.e. pash-1(mj100) [23097426]. | Worm | +40 | — | — |
| hsf-1 overexpression | Overexpression of hsf-1 extends the mean, median, and maximum lifespan by 37, 35, and 29% [22737090]. | Worm | +37 | +35 | +29 |
| age-1 RNAi | RNAi against age-1 extends lifespan by 30% [8700226; 8608934]. age-1 RNAi increases mean and maximum lifespan by 36-46% and 48-50% [12447374]. RNAi against age-1 increases mean lifespan by 83% [18828672]. age-1(mg44) zygotic null mutants have a mean (99%) and maximum (117%) lifespan extension [18828672]. | Worm | +36 to +99 | — | +48 to +117 |
| lmp-2 overexpression | Overexpression of lmp-2 increases mean, median and, maximum lifespan by 32, 35, and 41% [22737090]. | Worm | +32 | +35 | +41 |
| sod-1 overexpression | sod-1 overexpression increases mean, median, and maximum lifespan by 32, 25, and 35% [22737090]. | Worm | +32 | +25 | +35 |
| lars-2 mutation | A mutation that impairs mitochondrial function was associated with a longer lifespan. Mutation of lrs-2/lars-2(mg312) extends lifespan and is associated with impaired mitochondrial function. The recessive allele mg312 of lars-2 extends lifespan by 200% at 20 degree Celsius and 30% at 25 degree Celsius. Lifespan extension by mg312 was not dependent on daf-16(mgDf47). Homozygous lars-2(mg312) worms had multiple pleotropies like lower rates of growth, pumping and defecation as well as remain the size of early L4 worms and are sterile, with an arrested gonad that exhibited no germ-cell differentiation lars-2 is ubiquitously express, with prominent expression in body-wall muscle and neurons, with a mitochondrial subcellular localisation. Mitochondria of lars-2 are noticeably disorganized, swollen and sometimes fused. lars-2 animals have lower ATP content and oxygen consumption [12447374]. | Worm | +30 to +200 | — | — |
| SNCA overexpression | Transgenic lines overexpressing either human wild-type or mutant (A53T) forms of the SNCA (alpha-synclein) gene under a pan-neuronal promoter live on average about 25% longer, even in weak (m577) and strong (e1370) daf-2 mutant backgrounds, and exhibited decreased pharyngeal pumping and egg-laying. Wild-type SNCA crossed into eat-2(ad1113) does not significantly effect lifespan compared to that of the background strain. Pumping rate in wild-type SCNA and A53T SCNA overexpression mutants were less than control already at day 1 of adulthood. The attenuation of lifespan exptesion by SNCA overexpression by growing on thick bacterial lawns, suggests that DR may explain some fo the effects on lifespan. SCNA overexpression increases average lifespan by 21.3% (wild-type) and 16.3% (A53T) [16782295]. | Worm | +26 to +34 | — | +19 to +31 |
| lyz overexpression | Overexpression of lyz increases mean, median, and maximum lifespan by 26, 30 and 44% [22737090]. | Worm | +26 | +30 | +44 |
| INS overexpression | Expression of human insulin under an inducible heat shock promoter increases nematode lifespan by 25% and is also able to enhance the lifespan of daf-2 mutants [11274053]. | Worm | +25 | — | — |
| ins-1 overexpression | Increased dosage of ins-1 under its own promoter as well as a heat shock promoter increases lifespan by 25% and is also able to increase the lifespan of daf-2 mutants. Overexpression of ins-1 also causes an increase in dauer formation and can enhance the dauer formation of daf-2 mutants [11274053]. | Worm | +25 | — | — |
| sod1 overexpression | Overexpression of sod1 increases mean, median, and maximum lifespan by 21, 25, and 29% [22737090]. | Worm | +23 | +25 | +44 |
| daf-16 overexpression | Overexpression of wild-type DAF-16 modestly increases lifespan by 20% [11747825]. | Worm | +20 | — | — |
GenAge
GenDR
