| YNR066C deletion | Deletion of YNR066C decrease replicative lifespan by 50% in the alpha strain [19030232]. | Yeast | -50 | — | — |
| NPT1 deletion | NPT1 deletion decreases replicative lifespan by 50% [17482543] as well as chronological lifespan [17110466]. Deletion of NPT1 shortens the lifespan in W303R. Replicative lifespan extension of cdc25-10 mutation (assumed to act as a genetic DR-mimetic) is cancelled out by NPT1 deletion [11000115].
NPT1 mutation results in loss of telomere and rDNA silencing [10841563], an effect that is likely caused by a loss of SIR2 activty due to decreased NAD levels. Mutation of NPT1 is synthetical lethal with mutation of QPT1 [11000115]. | Yeast | -50 | — | — |
| CCS1 deletion | Deletion of CCS1 reduces replicative lifespan by 50% [17460215]. | Yeast | -50 | — | — |
| MPT5 deletion | Deletion of MPT5 shortens replicative lifespan by about 50% [9150138; 7859289]. MPT5 deletion decreases average chronological lifespan by 50%, which is rescued to the wild-type level by PKC1 overexpression [17172436].
MPT5 mutants are temperature sensitive [7845352], hypersensitive to mating pheromone [9154842], and null mutants exhibit increased silencing at telomeres and decreased rDNA silencing [9584615]. Deletion of MPT5 is synthetical lethal with mutation of either SWI4, SWI6, or CCR4 in an ssd1-d background [11805047]. MPT5 is required for relocalization of the SIR complex to the nucleolus in sir4-42 strain [7859289]. | Yeast | -50 | — | — |
| YHF1 deletion | Deletion of YFH1 decreases replicative lifespan by 50% [15130665]. | Yeast | -50 | — | — |
| YME1 deletion | Deletion of YME1 decreases chronological lifespan by 50% [17492370] as well as replicative lifespan by 45% in the alpha strain [18340043]. | Yeast | -45 to -50 | — | — |
| bar-1 mutation | BAR-1 may play a role in regulating daf-16 during dauer formation, particularly in conditions of oxidative stress as it directly interaction with DAF-16 and loss of bar-1 reduces activity of DAF-16 in dauer formation and lifespan. Deletion of bar-1 reduces mean (44%) and maximal (18%) lifespan, which is to a similar degree as seen to daf-16 mutants [15905404]. | Worm | -44 | — | -18 |
| ACO1 deletion | Deletion of ACO1 decreases mean chronological lifespan by 42 - 56% in diploid cells [21447998]. | Yeast | -42 to -56 | — | — |
| AKR1 deletion | Deletion of AKR1 decreases replicative lifespan by 40% in the alpha strain [18340043]. Replicative lifespan decreased by 50% in the alpha strain [19030232]. | Yeast | -40 to -50 | — | — |
| HAP5 deletion | Deletion of HAP5 shortens replicative lifespan by approximately 40%. This is not a premature aging phenotype as "old" HAP5 cells do not become premature sterile or exhibit other biomarkers of yeast aging [9271578]. HAP5 null mutants are unable to grow on a non-fermentable carbon source [7828851]. | Yeast | -40 | — | — |
| Msra knockout | Msra homozygous knockouts exhibit a 40% shorter lifespan than wild-type or heterozygotes (C57BL/6J). Msra -/- mice have enhanced sensitivity to oxidative stress, accumulatehigher levels of protein cabronyls, and demonstrate and atypical walking pattern [11606777]. | Mouse | -40 | — | — |
| RAD51 deletion | Rad51 mutations result in a 40% reduced mean replicative lifespan in strain PSY316 [10207108]. RAD51 is required for gene conversion, but not for repair of an HO-induced double-stranded break [8692957]. RAD51 deletion decreases formation of extrachromosomal rDNA circles [10207108]. | — | -40 | — | — |
| RIF deletion | Deletion of RIF1 decrease replicative lifespan by 40% [9275199]. RIF1 deletion increases telomere silencing and length [8319907; 1577274], and therefore likely recruits SIR2 from rDNA to the telomeres which result in lifespan shortening. The sir4-42 allele suppresses the short lifespan of a RIF1 mutant [9275199]. | — | -40 | — | — |
| SOD1 deletion | Deletion of SOD1 decreases replicative lifespan by 40% [17460215]. Cells with a deletion of SOD1 exhibit a profound defect in entry into and survival during stationary phase (i.e. chronological lifespan) in the W303-B strain [8647826; 10222047], which is suppressed by expression of human Bcl-2 [9199172]. Deletion of SOD1 shortens replicative lifespan by approximately 40%. The magnitude of the decrease in lifespan does not appear to dependent on oxygen concentration in the atmosphere [12020810]. Deletion of SOD1 shortens replicative lifespan [10547026] Deletion of SOD1 shortens replicative as well as chronological lifespan [10222047].
Hypersensitivity to oxygene and significantly decreased replicative lifespan of SOD1 deletion can be ameliorated by exogenous ascorbate. If acorbate's negative effects of auto-oxidation are prevented by exchange of medium, ascorbate prolongs mean and maximum replicative lifespan in the atmosphere of air and pure oxygene [15621721].
SOD1 deletion causes sensitivity to hyperoxia as well as methionine and lysine auxotrohies [9199172]. | Yeast | -40 | — | — |
| YBR225W deletion | Deletion of YBR225W decreases replicative lifespan by 40% in the alpha strain [19030232]. | Yeast | -40 | — | — |
| ARP1 deletion | Deletion of ARP1 decreases replicative lifespan by 40% in the alpha strain [18340043; 19030232]. | Yeast | -40 | — | — |
| Hsp22 transposition | Animals that do not express Hsp22 (due to a transposition into its transcriptional starting site) have a 40% decrease in lifespan, exhibit a 30% decrease in locomotor activity and are sensitive to mild stress [20036725]. | Fly | -40 | — | — |
| NTH2 deletion | Deletion of NTH2 shortens mean chronological lifespan by 39% (at 30 degree Celsus in BY4742) [22783207].
NTH2 mutant cells have elevated trehalose concentration before they enter the non-proliferative stationary growth phase which remained high during the stationary phase. NTH2 deletion cells have no altered ROS levels in pre-quiescent cells [22783207]. | Yeast | -39 | — | — |
| BCY1 deletion | Disruption in BCY1 by mutation results decreases mean and maximum replicative lifespan by 37 and 16% and is associated with increased PKA activity [8195187]. | Yeast | -37 | — | -16 |
| CHL1 deletion | CHL1 deletion mutant exhibits a shortened mean and maximum lifespan by 36 and 29%, respectively, as well as hypersensitivity to heat stress. CHL1 may modulate transcriptional silencing in the presence of Sir proteins [16182251]. | Yeast | -36 | — | -29 |
| GTR1 deletion | GTR1 deletion decreases mean and maximum replicative lifespan under AL by 36 and 51%, respectively, and cancels out the lifespan extending effect of DR [22912585]. | Yeast | -36 | — | -51 |
| YOL092W deletion | Deletion of YOL092W decreases mean and maximum replicative lifespan by 36 and 21%, respectively. Lifespan of YOL092Y deletion mutants is extended by 0.5% glucose restriction [22912585]. | Yeast | -36 | — | -21 |
| ERG5 deletion | Deletion of ERG5 decreases replicative lifespan by 35% in the a strain [18340043], but increases mean chronological lifespan by 26 - 116% (26, 40, 43, 62, 116) in diploid cells [21447998]. Deletion of ERG5 cancels out the replicative lifespan extension of 0.5% glucose restriction [18690010]. | Yeast | -35 to +116 | — | — |
| MTC5 deletion | Deletion of MTC5 decreases replicative lifespan by 35% in the alpha strain [19030232]. | Yeast | -35 | — | — |
| YDR132C deletion | Deletion of YDR132C decreases replicative lifespan by 35% in the alpha strain [19030232]. | Yeast | -35 | — | — |
GenAge
GenDR
