| prx-13 | PeRoXisome assembly factor | Knockdown of prx-13 increases mean and maximum lifespan by 17% (28%!) and 11% respectively. prx-13 knockdown from hatching does no effect [22509016]. | Nematode |
| prx-11 | PeRoXisome assembly factor | RNAi against prx-11 increases mean and maximum lifespan 11% [22509016]. | Nematode |
| prx-5 | PeRoXisome assembly factor | Postdevelopmental RNAi kockdown of prx-5 in the enhanced RNAi strain eri-1(mg366) increases mean lifespan by 30% [17411345]. Knockdown of prx-5 initiated on the first day of adulthood increases mean and maximum lifespan by 8% (6%!) and 11% respectively. prx-5 knockdown from hatching has no effect on lifespan or slightly shortened lifespan [22509016]. This might indicate that prx-5 is important during development but may exert postedevelopmental detrimental effects on longevity [22509016]. | Nematode |
| F18F11.1 | — | F18F11.1 knockdown extends mean lifespan by 17% and 11%, respectively [22509016]. | Nematode |
| bub-3 | yeast BUB homolog | RNAi against bub-3 increases mean and maximum lifespan by 6-7% and 7%, respectively [18059442]. | Nematode |
| fzy-1 | FiZzY (CDC20 protein family) homolog | RNAi against fzy-1 increases mean and maximum lifespan by 17-32% and 14%, respectively [18059442]. | Nematode |
| R03D7.4 | — | RNAi against R03D7.4 increases mean and maximum lifespan by 9-17% and 7%, respectively [18059442]. | Nematode |
| ncbp-2 | Nuclear Cap-Binding Protein | RNAi against ncbp-2 decreases mean and maximum lifespan by 23-25% and 7%, respectively [18059442]. | Nematode |
| mrpl-47 | Mitochondrial Ribosomal Protein, Large | RNAi against mrpl-47 extends mean and maximum lifespan by 18% and 50%, respectively [12447374]. This lifespan extension is not suppressed by daf-16 mutation (mgDF74). mrpl-47 knockdown results in lower ATP content and oxygen consumption [12447374]. | Nematode |
| F13G3.7 | — | RNAi against F13G3.7 increases mean and maximum lifespan by 25% and 50%, respectively. Lifespan extension by F13G3.7 RNAi is dependent on daf-16 [12447374]. | Nematode |
| wah-1 | Worm AIF (apoptosis inducing factor) Homolog | wah-1 RNAi in the adulthood extends the lifespan [New longevity regulators]. RNAi against wah-1 started after the animal reaches the late L4 stage extends mean lifespan by 7-8% [22103665]. | Nematode |
| ucr-1 | Ubiquinol-Cytochrome c oxidoReductase complex | RNAi against ucr-1 increases mean lifespan by 20% [17608836]. ucr-1 RNAi started after the animal reached the late L4 stage increase mean lifespan by 8-14% [22103665]. | Nematode |
| F09F7.4 | NEK (NEver in mitosis Kinase) Like | F09F7.4 RNAi in the adulthood extends the lifespan [New longevity regulators]. RNAi against F09F7.4 started after the animal reached the late L4 stage increases mean lifespan by 6-12% [22103665]. | Nematode |
| rrt-1 | aRginyl aa-tRNA syntheTase | rrt-1 RNAi in the adulthood extends the lifespan [New longevity regulators]. Kockdown of rrt-1 via RNAi started after the animal reached the late L4 stage increases lifespan by 15-27% [22103665]. | Nematode |
| eft-2 | Elongation FacTor | Post-developmental RNA interference reduces mean lifespan by 15% [17673661]. Knockdown of eft-2 started after the animal reached the late L4 stage increases mean lifespan by 6-27% and 8%, respectively [22103665]. | Nematode |
| mdh-1 | Malate DeHydrogenase | RNAi against mdh-1 decreases median lifespan by 10% in wild type animals and by 16% in daf-2 mutants [18006689]. mdh-1 RNAi started after the animal reached the late L4 stage increases mean and maximum lifespan by 4-27% and 9% [22103665]. | Nematode |
| daf-8 | abnormal DAuer Formation | daf-8 mutation in adults increases mean lifespan by 9-31% but it did not increase maximum lifespan [17900898]. | Nematode |
| daf-14 | abnormal DAuer Formation | daf-14(m77) mutation increases mean lifespan by 21-44% and maximum lifespan by 29% [17900898]. | Nematode |
| daf-7 | abnormal DAuer Formation | daf-7 mutation does not significantly change lifespan [8247153]. Mutations in daf-7 cause up to 50% mean and maximum life-extension. This effect is dependent upon daf-3 and on daf-16 but independent of daf-2. daf-7(e1372) increases mean and maximum lifespan by 13-39% and 55%, respectively. daf-7(m62) increases mean and maximum lifespan by 20-29% and 29% [17900898]. RNAi against daf-7 only in the adulthood increases mean and maximum lifespan by 26 and 7%, respectively [17900898]. | Nematode |
| ragc-1 | RAs-related GTP binding protein C homolog | ragc-1 RNAi only during adulthood increases mean, median and maximum lifespan by 13-33, 21 and 33-37% respectively, but failed so in skn-1 or daf-16 mutant (with and without FUdR). Interference with TORC1 by ragc-1 RNAi increases heat resistance in daf-16 and skn-1 mutants, but not not in skn-1;daf-16 double mutant. ragc-1 RNAi increases lifespan in a strain in which RNAi is restricted to the intestine (VP288). RNAi of ragc-1 decreases overall mRNA translation but increases autophagy (measured by LGG-1 puncta) [22560223]. | Nematode |
| raga-1 | RAs-related GTP-binding protein A | raga-1 RNAi only during adulthood increases mean, median, 75th %ile and maximum lifespan by 9-42, 26-28, 16-52 and 35%, respectively, but failed so in skn-1 or daf-16 mutant (with and without FUdR). Lifespan of the skn-1;daf-16 mutant was unaffected by raga-1 RNAi. Knockdown of raga-1 dramatically increases stress tolerance in an skn-1 but not daf-16-dependent manner [22560223]. | Nematode |
| rict-1 | RICT0r (Rapamycin-Insensitive Companion of TOR) homolog | C. elegans with mutations in the TORC2 complex gene rict-1 (Rictor) grow slowly and have small body size, and live slightly longer than wild-type when maintained on ârichâ food such as the RNAi feeding strain HT115 and at elevuated temperature (25 degree Celsius) [Soukas et al., 2009 in (Robida-Stubbs et al., 2012)]. rict-1 RNAi at 20 degree Celsius in the adulthood increases mean, median, 75th %ile and maximum lifespan by 12-42, 22-29, 13-32 and 28-54%, respectively, dependent on skn-1. daf-16 is not required for lifespan to be increased by rict-1 RNAi, or when TORC1 and TORC2 are blocked by ragc-1;rict-1 RNAi. rict-1 RNAi extends mean, median, 75th %ile and maximum lifespan in the intestine-specific RNA stains VPS288 by 18, 19, 18 and 16%, respectively [22560223]. | Nematode |
| rsks-1 | RSK-pSeventy (RSK-p70 kinase) homolog | RNA interference reducing S6K (rsks-1) mRNA levels by about two-fold extends mean lifespan 13-47% and maximum lifespan by up to 60%. Deletion mutants also live longer. A life-extending effect was also observed in daf-16 mutants and sir-2.1 mutants but not in daf-2 mutants. TOR RNA interference further extends lifespan of rsks-1 mutants [17266679]. DAF-16 reduces expression of rsks-1 [15253933;22560223]. Autophagy is reduced by knockdown of S6 kinase RSKS-1 [22560223], as it increases translation downstream of TORC1 but also promotes autophagy [15296714]. | Nematode |
| hcf-1 | Host Cell Factor-1 | hcf-1 is conserved protein [12826401], ubiquitously expression in C. elegans under normal culture conditions and predominantly nuclear [7876203]. HCF-1 forms a complex with DAF-16. hcf-1 inactivation by mutation cause a daf-16-dependent lifespan extension of up to 40% and heightened resistance to specific stimuli. The hcf-1(ok559) mutation increases mean and maximum lifespan by 10-37 and 29%, while the strong hcf-1(pk924) mutation extends mean and maximum lifespan by 29-31 and 53-88%, respectively. In the absence of hcf-1 there is a greater enrichment of DAF-16 at its target gene promoters and more robust DAF-16-mediated regulation of selective transcriptional targets. hcf-1 mutation extends lifespan of glp-1(e2141) mutants which lack germline cells, [18828672].
SKN-1 contributes to the enhanced oxidative stress resistance incurred by hcf-1 mutation. HCF-1 prevents the nuclear accumulation of SKN-1 and represses the transcriptional activation of SKN-1 specifically at target genes involved in cellular detoxification [22568582]. | Nematode |
| nac-3 | NADC (Na+-coupled dicarboxylate transporter) family | RNA interference knockdown of nac-3 leads to an 15% increase in average lifespan. | Nematode |
