Authors: Ewbank JJ; Barnes TM; Lakowski B; Lussier M; Bussey H; Hekimi S
Abstract: Mutations in the Caenorhabditis elegans gene clk-1 affect biological timing and extend longevity. The gene clk-1 was identified, and the cloned gene complemented the clk-1 phenotypes and restored normal longevity. The CLK-1 protein was found to be conserved among eukaryotes, including humans, and structurally similar to the yeast metabolic regulator Cat5p (also called Coq7p). These proteins contain a tandem duplication of a core 82-residue domain. clk-1 complemented the phenotype of cat5/coq7 null mutants, demonstrating that clk-1 and CAT5/COQ7 share biochemical function and that clk-1 acts at the level of cellular physiology.Keywords: Amino Acid Sequence; Animals; Caenorhabditis elegans/*genetics/metabolism/*physiology; *Caenorhabditis elegans Proteins; Cell Aging/*genetics; Chromosome Mapping; Conserved Sequence; Exons; Fungal Proteins/chemistry/genetics/physiology; *Genes, Helminth; Genetic Complementation Test; Glycerol/metabolism; Helminth Proteins/chemistry/*genetics/physiology; Humans; Longevity/genetics; Mice; Molecular Sequence Data; Phenotype; Protein Structure, Secondary; Protein Structure, Tertiary; RNA Splicing; *Saccharomyces cerevisiae Proteins
Journal: Science (New York, N.Y.)
Date: Feb. 14, 1997
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Ewbank JJ, Barnes TM, Lakowski B, Lussier M, Bussey H, Hekimi S (1997) Structural and functional conservation of the Caenorhabditis elegans timing gene clk-1. Science (New York, N.Y.) 275: 980-3.