Technologies

  • Affymetrix Genome-Wide Human SNP Array 6.0
  • PCR
  • Multilocus PCR
  • ELISA
  • AP-PCR
  • Illumina Infinium HD Human660W-Quad BeadChips; Illumina Infinium II HumanHap550-Duo BeadChips
  • SNPlex technology
  • PCR-RFLP
  • GWAS
  • PCR, gel electrophoresis
  • ABI PRISMw 3700 DNA Analyzer (Applied Biosystems)
  • N/A
  • Dynamic allele specific hybridization (DASH)
  • Applied Biosystems 3730 automated DNA sequencer
  • Taqman Real-Time PCR allelic discrimination assay
  • 373A automated DNA sequencer
  • SNPlexTM Genotyping System and TaqMan1 SNP Genotyping Assays
  • PCR, ABIPRISM 310 Genetic Analyzer
  • "BigDye Terminator cycle ""sequencing kit and an ABI Prism 3700 DNA analyzer or by real-time
  • PCR and gel electrophoresis
  • TaqMan SNP genotyping assay
  • PCR, single-base extension (SBE)
  • Taqman, PSQ HS 96A Pyrosequencer
  • PCR, restriction enzyme digestion, gel electrophoresis
  • Two-dimensional gene scanning (TDGS)
  • "Illumina GoldenGate, Sequenom MassARRAY
  • Illumina Infinium HD Human660W-Quad BeadChips and Illumina OmniExpress BeadChips
  • PCR & gel electrophoresis
  • PCR-mediated site-directed mutagenesis
  • Illumina GoldenGate
  • PCR, TaqMan
  • Real time PCR on LightCycler system
  • Affymetrix HG-Focus arrays
  • RFLP-PCR, gel electrophoresis
  • PCR-RFLP, restriction digest, gel electrophoresis
  • "Genotyping data downloaded from the International Hap-
  • PCR, restriction digest, gel electrophoresis
  • Nanogen system
  • Illumina 550K array
  • PE310 DNA sequencer
  • Restriction fragment analysis
  • Illumina GoldenGate platform
  • Affymetrix 100K SNP GeneChip
  • SDS-PAGE, immunoblotting (genotype inferred from protein isoforms)
  • Arbitrarily primed polymerase chain reaction (AP-PCR) in combination with single-stranded DNA conformation polymorphism (SSCP)
  • isoelectric focusing of plasma.
  • PCR & sequencing
  • Reverse dot-blot (Murex Diagnostics Inno-Lipa DRB1)
  • PCR and restriction analysis
  • PCR & gel electropheresis
  • Illumina BeadChip 300K system
  • Allele-specific oligonucleotide hybridization
  • Restriction fragment length polymorphism
  • ARMS-PCR assay
  • deCODE Genetics 1100 microsatellite marker set
  • Amplification-refractory mutation system method
  • Multiplex PCR
  • PCR-based SNP genotyping (Applied Biosystems)
  • PCR-SSP (Genovision)
  • PCR, RFLP, gel electropheresis
  • SNPlex Genotyping System (Applied Biosystems)
  • 3130xl Genetic Analyzer (Applied Biosystems)
  • Illumina BeadChips
  • Not described
  • Taqman Allelic Discrimination
  • PCR, gel electrophoresis, sequencing
  • Not reported
  • TagSNP genotyping, Sequenom MASSarray
  • RFLP-PCR
  • ABI Prism 310 Genetic Analyser
  • HumanHap BeadChip 317K duo system (Illumina)
  • Allele-specific PCR
  • PCR LightCycler (Roche)
  • Affymetrix Human SNP Array 6.0
  • Affymetrix 50K Human Gene Focused Array
  • Reanalysis of FHS and FHSO cohort data from dbgap. Three SNPs correlate with surivival (follow-up data).
  • ARMS-PCR
  • Applied Biosystems 7700 Sequence Detector
  • Microdroplet lymphocyte cytotoxity test
  • "Illumina
  • ABI Prism Linkage Mapping Set
  • SNPstream 48plex (Beckman Coulter)
  • Not given
  • llumina HumanLinkage-12 Genotyping BeadChip.
  • GeneChip Human Mapping 10K 2.0 Array (Affymetrix)
  • ABI SNaPshot kit
  • plasma electropheresis
  • Amplifluor
  • MT full genome sequencing
  • Kaspar and Taqman arrays
  • unknown (old paper)
  • Sequenom MassARRAY
  • PCR, Illumina GoldenGate Technology
  • Affymetrix 6.0 GeneChip Human Mapping 1 million SNP array set
  • Illumina 610 QuadChip, HumanHap300
  • BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems)
  • SCCP, GIBCO BRL, autoradiography, ABI Fluorescent Sequencing System, others
  • SNPlex technology (Applied Biosystems)
  • SAS system (Proc-LogXact 5), SAS FREQ procedure (version 8.2), Proc-StatXact (version 4.0.1)
  • KASPar (fluorescence-based competitive allele-specific PCR system assay), COMPARE2 software
  • PCR-based sequencing
  • sequence specific probes
  • Arlequin Software
  • PCR based probes
  • TaqMan1 SNP Genotyping Assay (Applied Biosystems)
  • Biometra T-gradient thermal-cycler
  • Sequencing
  • Two-dimensional gene scanning (TDGS) technology platform
  • PCR based method
  • Illumina Infinium HD Human660W-Quad BeadChips, Illumina OmniExpress BeadChips
  • Agilent SureSelect in-solution target capture, Agilent eArray, BWA version 0.5.9, Picard, Genome Analysis Toolkit (GATK), iPLEX MassArray assays, MassARRAY Typer, SNP & Variation suite version 7.6.11
  • Restrictive enzyme Xba I, Taq polymerase
  • Taq Polymerase (CenBiot-RS), restriction enzyme Hha I (Gibco)
  • Taq polymerase
  • SNPIex Genotyping System, TaqMan SNP Genotyping Assays, Infinium II Assay-HumanHap 317K duo BeadChip system
  • BigDye Terminator cycle sequencing kit, ABI Prism 3700 DNA analyzer, Sequencher, PSQ 96, SNP reagent kit, SNPAlyze
  • QIAamp Blood Kit (Qiagen)
  • Assay-by-Design, qPCR core kit, Sequenom MassARRAYtm
  • HhaI restriction enzyme (Promega)
  • Taq-polymerase
  • Wizard genomic DNA extraction kit (Promega)
  • Taq polymerase (Gibco)
  • Taq polymerase, HindIII enzyme, EZ DNA Methylation-Gold Kit, pGEM-T vector, LightCycler 480 Instrument II, LightCycler 480 Sybr Green I Master Kit, Statistica
  • LightCycler system (Roche Applied Sciences), GAUSS programming package
  • AB6100, UV-spectrophotometer, Nla III, capillary electrophoresis unit, Ultra Sensitive ELISA Kit, G*Power 3.03
  • HaploView software, QIAamp DNA Mini and Micro Kits, Illumina GoldenGate platform, R statistical environment
  • I'm not sure exactly. My best guess is some sort of protein analysis that allows to differentiate between different alleles of the gene
  • Peripheral blood lymphocytes using a micro-technique (Mittal et al. 1968)
  • PCR-VNTR (variable number of tandem repeats)
  • antigens were detected according to the standard microdroplet lymphocyte cytotoxity test
  • PCR, Amp-FLP
  • allele-specific PCR, long PCR
  • Oligonucleotide ligation assay
  • Allele classification was performed by using the same allele ladder in each experiment. The ladder was obtained by PCR amplification of reference DNA samples containing 3'APOB-VNTR alleles of known size.
  • PCR for segment, located in a repetitive Alu sequence in intron 16
  • PCR-RFLP_x000D_
  • CfoI Restriction site to PCR products, on 8% polyacrylamide gel with silver staining method
  • PCR and subsequent electrophoretic analysis on a 2 % agarose gel
  • PCR-RFLP. Amplicons were digested by HhaI and genotypes were determined after electrophoresis on a 10 % polyacrylamide gel stained with ethidium bromide
  • ABI Prism Linkage Mapping Set, Version 2 (Applied Biosystems), and True Allele PCR Premix (Applied Biosystems)
  • PCR products were electrophoresed in a 2% agarose gel. molecular weight markers Markers VI and XIV (Roche) were employed, together with ad hoc molecular weight standards comprising common and rare HRAS1 3'VNTR alleles whose size was verified by autom
  • MvaI restriction enzyme, loaded on 3% agarose.
  • PE ABI Prism 310 genetic analyzer (Perkin Elmer)
  • FokI restriction enzyme, loaded on 3% agarose.
  • HincII restriction enzyme, loaded on 3% agarose.
  • PCR-restriction fragment length polymorphism analysis
  • MspI Restriction analysis after PCR- amplification of the DNA regions containing the restriction sites
  • SstI Restriction analysis after PCR- amplification of the DNA regions containing the restriction sites
  • HincII Restriction analysis after PCR- amplification of the DNA regions containing the restriction sites
  • PCR and sequence specific primers as previously reported --Lio, D., Balistreri, C.R., Colonna-Romano, G., Motta, M., Fran- ceschi, C., Malaguarnera, M., Candore, G., Caruso, C., 2002. Association between the MHC class I gene HFE polymorphisms and lon
  • PCR and sequence specific primers as previously reported --Lio, D., Balistreri, C.R., Colonna-Romano, G., Motta, M., Fran- ceschi, C., Malaguarnera, M., Candore, G., Caruso, C., 2002. Association between the MHC class I gene HFE polymorphisms and lon
  • PCR and sequence specific primers as previously reported --Lio, D., Balistreri, C.R., Colonna-Romano, G., Motta, M., Fran- ceschi, C., Malaguarnera, M., Candore, G., Caruso, C., 2002. Association between the MHC class I gene HFE polymorphisms and lon
  • PCR products were separated by electrophoresis on a 1.5% agarose gel
  • Multilocul PCR
  • using the molecular weight (size: 22 bp) difference between the deleted and non-deleted forms of the 2DS4 gene, via gel electrophoresis on a 2% Metaphor agarose gel. non-deleted were confirmed as by employing a sequence specific oligonucleotide prob
  • Taqman Universal Master Mix (Applied Biosystems)
  • ABI 377 automatic sequencer (Applied Biosystems)
  • loaded on 3% agarose (presence/absence of PCR product)
  • Msp1 restriction enzyme, loaded on 3% agarose.
  • BstU restriction enzyme, loaded on 3% agarose.
  • PCR - loaded on 3% agarose. A fragment of 401 bp, or 417 bp if the 16 bp duplication is present
  • PCR restriction fragment length polymorphism. The fragments were separated on agarose gel.
  • Taqman-based allelic discrimination assay
  • Taqman- based allelic discrimination assay. ABI Prism 7700 (Applied Biosystems)
  • DNA sequencing using the BigDye Terminator cycle sequencing kit and an ABI Prism 3700 DNA analyzer or by real-timepyrophosphate DNA sequencing using a PSQ 96 system
  • Taqman Allelic Discrimination method on an Applied Biosystems 7700 Sequence Detector
  • Polymerase chain reaction sequence specific oligonucleotide (PCR-SSO) reverse dot blot using the Dynal RELI SSO system (Hoffman-La Roche Ltd. and Roche Molecular Systems, Inc., Alameda, CA)
  • PCR fragment amplified using NcoI restriction enzyme, loaded on 2% agarose
  • PCR and Hha I restriction enzyme digestion on 5% agarose gel and ethidium bromide staining
  • restriction fragment length polymorphism (RFLP)
  • A multilocus PCR
  • Specific PCR of YTHDF2 (TG)12-27 Polymorphism, RT PCR
  • Direct sequencing analysis of PCR fragments using BigDyeTerminatorTM protocol on an automated 3100ABI Prism Genetic Analyzer (Applied Biosystem, Foster City, CA)
  • MwoI restriction enzyme
  • Allele specific PCR products were detected by electrophoresis on 2% agarose
  • DYEnamic ET Terminator Cycle Sequencing Premix Kit (Amersham Pharmacia Biotech, Piscataway, NJ, USA) on an ABI Prism 3730xl Genetic Analyser (Applied Biosystems, Foster City, CA, USA)
  • ABI Prism 7000 Sequence Detection System
  • PCR amplification of short mtDNA fragments, followed by restriction enzyme analysis (RFLP) and confirmed by HVR-I and HVR-II sequencing
  • Taqman SNP Genotyping Assays (Applied Biosystems) on an automated_x000D_ platform
  • ABI Prism 7700 Sequence Detector (Applied Biosystems)
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
  • SNaPshot minisequencing reaction, PCR
  • genomic DNA was analyzed on the Illumina 370 CNV chip
  • polymerase chain reaction-restriction fragment length polymorphism reaction-restriction fragment length polymorphism (PCR-RFLP)
  • "VDR genotyping followed protocols described by de la Torre et al. (2008)" doi: 10.1086/525043. Followed the article: _x000D_http://jid.oxfordjournals.org/content/197/3/405/F3.expansion.html, Which led me to believe they used:_x000D_ ABI-PRISM 3100 G
  • "VDR genotyping followed protocols described by de la Torre et al. (2008)" doi: 10.1086/525043. Followed the article: _x000D_http://jid.oxfordjournals.org/content/197/3/405/F3.expansion.html, Which led me to believe they used:_x000D_ ABI-PRISM 3100 G
  • "VDR genotyping followed protocols described by de la Torre et al. (2008)" doi: 10.1086/525043. Followed the article: _x000D_http://jid.oxfordjournals.org/content/197/3/405/F3.expansion.html, Which led me to believe they used:_x000D_ ABI-PRISM 3100 G
  • "VDR genotyping followed protocols described by de la Torre et al. (2008)" doi: 10.1086/525043. Followed the article: _x000D_http://jid.oxfordjournals.org/content/197/3/405/F3.expansion.html, Which led me to believe they used:_x000D_ ABI-PRISM 3100 G
  • "VDR genotyping followed protocols described by de la Torre et al. (2008)" doi: 10.1086/525043. Followed the article: _x000D_http://jid.oxfordjournals.org/content/197/3/405/F3.expansion.html, Which led me to believe they used:_x000D_ ABI-PRISM 3100 G
  • genome-wide SNP data selected from the Illumina control database
  • RFLP
  • Hot start PCR
  • SNPlexTM Genotyping System (Applied Biosystems)
  • PCR-based restriction fragment length polymorphism (RFLP) analysis
  • ABI Prism 310 genetic analyzer; Applied Biosystems & replicared with low-density DNA microarray based on allele-specific probes
  • Genomic PCR_x000D_ and BclI-RFLP analysis. DdeI restriction enzyme.
  • Genomic PCR_x000D_ and BclI-RFLP analysis.
  • DdeI restriction enzyme.
  • TaqMan SNP genotyping assays
  • Must purchase article to view
  • PSQ HS 96A Pyrosequencer
  • Sequenced in ABI 3730 DNA analyser (Applied Biosystems)
  • allele-specific PCR-based KASPar SNP genotyping system (KBiosciences, Hoddesdon, UK) (Cuppen
  • genotyped on a Sequenom MassArray iPLEX platform
  • Protocol of BigDye v1.1 kit and an ABI 3130XL sequencing system (Applied Biosystems)
  • DNA sequencing
  • Each country by one of the following: Illumina Infinium HD Human660W-Quad BeadChips, Illumina HumanOmniExpress BeadChips, Illumina Infinium HD Human610-Quad BeadChips
  • Illumina 610 Quad Array
  • Immunobloting
  • Sentrix Array Matrices
  • Affymetrix 500K and 50K Mapping Arrays
  • Sequenom's chip-based matrix-assisted laser desorption/ionization time-of-flight MS and RT-PCR
  • Western Blotting
  • Version 3 Illumina Infinium II HumanHap550 SNP chip array, Illumina 370CNV, Affymetrix GeneChip SNP Array 6.0, Illumina 370CNV, Illumina 550K, Illumina 550K, Illumina Human1M-Duo, Affymetrix SNP Array 6.0, respectively.
  • Sequenom's chip-based DNA MASSARRAY
  • Illumina: 370 CNV chip v1.0, Human610-Quad v1.0, Human 1 M v1.0
  • Meta-analysis
  • Serological tests
  • ABI-PRISM 3100 G
  • P value reported is uncorrected, but association did remain significant after correcting for multiple testing. P value for association with age at death.
  • P value for association with age at death.
  • Illumina 300, Illumina 610
  • Finding confirmed in replication cohort.
  • Illumina HumanHap BeadChip 317K duo system