Technologies
Affymetrix Genome-Wide Human SNP Array 6.0
PCR
Multilocus PCR
ELISA
AP-PCR
Illumina Infinium HD Human660W-Quad BeadChips; Illumina Infinium II HumanHap550-Duo BeadChips
SNPlex technology
PCR-RFLP
GWAS
PCR, gel electrophoresis
ABI PRISMw 3700 DNA Analyzer (Applied Biosystems)
N/A
Dynamic allele specific hybridization (DASH)
Applied Biosystems 3730 automated DNA sequencer
Taqman Real-Time PCR allelic discrimination assay
373A automated DNA sequencer
SNPlexTM Genotyping System and TaqMan1 SNP Genotyping Assays
PCR, ABIPRISM 310 Genetic Analyzer
"BigDye Terminator cycle ""sequencing kit and an ABI Prism 3700 DNA analyzer or by real-time
PCR and gel electrophoresis
TaqMan SNP genotyping assay
PCR, single-base extension (SBE)
Taqman, PSQ HS 96A Pyrosequencer
PCR, restriction enzyme digestion, gel electrophoresis
Two-dimensional gene scanning (TDGS)
"Illumina GoldenGate, Sequenom MassARRAY
Illumina Infinium HD Human660W-Quad BeadChips and Illumina OmniExpress BeadChips
PCR & gel electrophoresis
PCR-mediated site-directed mutagenesis
Illumina GoldenGate
PCR, TaqMan
Real time PCR on LightCycler system
Affymetrix HG-Focus arrays
RFLP-PCR, gel electrophoresis
PCR-RFLP, restriction digest, gel electrophoresis
"Genotyping data downloaded from the International Hap-
PCR, restriction digest, gel electrophoresis
Nanogen system
Illumina 550K array
PE310 DNA sequencer
Restriction fragment analysis
Illumina GoldenGate platform
Affymetrix 100K SNP GeneChip
SDS-PAGE, immunoblotting (genotype inferred from protein isoforms)
Arbitrarily primed polymerase chain reaction (AP-PCR) in combination with single-stranded DNA conformation polymorphism (SSCP)
isoelectric focusing of plasma.
PCR & sequencing
Reverse dot-blot (Murex Diagnostics Inno-Lipa DRB1)
PCR and restriction analysis
PCR & gel electropheresis
Illumina BeadChip 300K system
Allele-specific oligonucleotide hybridization
Restriction fragment length polymorphism
ARMS-PCR assay
deCODE Genetics 1100 microsatellite marker set
Amplification-refractory mutation system method
Multiplex PCR
PCR-based SNP genotyping (Applied Biosystems)
PCR-SSP (Genovision)
PCR, RFLP, gel electropheresis
SNPlex Genotyping System (Applied Biosystems)
3130xl Genetic Analyzer (Applied Biosystems)
Illumina BeadChips
Not described
Taqman Allelic Discrimination
PCR, gel electrophoresis, sequencing
Not reported
TagSNP genotyping, Sequenom MASSarray
RFLP-PCR
ABI Prism 310 Genetic Analyser
HumanHap BeadChip 317K duo system (Illumina)
Allele-specific PCR
PCR LightCycler (Roche)
Affymetrix Human SNP Array 6.0
Affymetrix 50K Human Gene Focused Array
Reanalysis of FHS and FHSO cohort data from dbgap. Three SNPs correlate with surivival (follow-up data).
ARMS-PCR
Applied Biosystems 7700 Sequence Detector
Microdroplet lymphocyte cytotoxity test
"Illumina
ABI Prism Linkage Mapping Set
SNPstream 48plex (Beckman Coulter)
Not given
llumina HumanLinkage-12 Genotyping BeadChip.
GeneChip Human Mapping 10K 2.0 Array (Affymetrix)
ABI SNaPshot kit
plasma electropheresis
Amplifluor
MT full genome sequencing
Kaspar and Taqman arrays
unknown (old paper)
Sequenom MassARRAY
PCR, Illumina GoldenGate Technology
Affymetrix 6.0 GeneChip Human Mapping 1 million SNP array set
Illumina 610 QuadChip, HumanHap300
BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems)
SCCP, GIBCO BRL, autoradiography, ABI Fluorescent Sequencing System, others
SNPlex technology (Applied Biosystems)
SAS system (Proc-LogXact 5), SAS FREQ procedure (version 8.2), Proc-StatXact (version 4.0.1)
KASPar (fluorescence-based competitive allele-specific PCR system assay), COMPARE2 software
PCR-based sequencing
sequence specific probes
Arlequin Software
PCR based probes
TaqMan1 SNP Genotyping Assay (Applied Biosystems)
Biometra T-gradient thermal-cycler
Sequencing
Two-dimensional gene scanning (TDGS) technology platform
PCR based method
Illumina Infinium HD Human660W-Quad BeadChips, Illumina OmniExpress BeadChips
Agilent SureSelect in-solution target capture, Agilent eArray, BWA version 0.5.9, Picard, Genome Analysis Toolkit (GATK), iPLEX MassArray assays, MassARRAY Typer, SNP & Variation suite version 7.6.11
Restrictive enzyme Xba I, Taq polymerase
Taq Polymerase (CenBiot-RS), restriction enzyme Hha I (Gibco)
Taq polymerase
SNPIex Genotyping System, TaqMan SNP Genotyping Assays, Infinium II Assay-HumanHap 317K duo BeadChip system
BigDye Terminator cycle sequencing kit, ABI Prism 3700 DNA analyzer, Sequencher, PSQ 96, SNP reagent kit, SNPAlyze
QIAamp Blood Kit (Qiagen)
Assay-by-Design, qPCR core kit, Sequenom MassARRAYtm
HhaI restriction enzyme (Promega)
Taq-polymerase
Wizard genomic DNA extraction kit (Promega)
Taq polymerase (Gibco)
Taq polymerase, HindIII enzyme, EZ DNA Methylation-Gold Kit, pGEM-T vector, LightCycler 480 Instrument II, LightCycler 480 Sybr Green I Master Kit, Statistica
LightCycler system (Roche Applied Sciences), GAUSS programming package
AB6100, UV-spectrophotometer, Nla III, capillary electrophoresis unit, Ultra Sensitive ELISA Kit, G*Power 3.03
HaploView software, QIAamp DNA Mini and Micro Kits, Illumina GoldenGate platform, R statistical environment
I'm not sure exactly. My best guess is some sort of protein analysis that allows to differentiate between different alleles of the gene
Peripheral blood lymphocytes using a micro-technique (Mittal et al. 1968)
PCR-VNTR (variable number of tandem repeats)
antigens were detected according to the standard microdroplet lymphocyte cytotoxity test
PCR, Amp-FLP
allele-specific PCR, long PCR
Oligonucleotide ligation assay
Allele classification was performed by using the same allele ladder in each experiment. The ladder was obtained by PCR amplification of reference DNA samples containing 3'APOB-VNTR alleles of known size.
PCR for segment, located in a repetitive Alu sequence in intron 16
PCR-RFLP_x000D_
CfoI Restriction site to PCR products, on 8% polyacrylamide gel with silver staining method
PCR and subsequent electrophoretic analysis on a 2 % agarose gel
PCR-RFLP. Amplicons were digested by HhaI and genotypes were determined after electrophoresis on a 10 % polyacrylamide gel stained with ethidium bromide
ABI Prism Linkage Mapping Set, Version 2 (Applied Biosystems), and True Allele PCR Premix (Applied Biosystems)
PCR products were electrophoresed in a 2% agarose gel. molecular weight markers Markers VI and XIV (Roche) were employed, together with ad hoc molecular weight standards comprising common and rare HRAS1 3'VNTR alleles whose size was verified by autom
MvaI restriction enzyme, loaded on 3% agarose.
PE ABI Prism 310 genetic analyzer (Perkin Elmer)
FokI restriction enzyme, loaded on 3% agarose.
HincII restriction enzyme, loaded on 3% agarose.
PCR-restriction fragment length polymorphism analysis
MspI Restriction analysis after PCR- amplification of the DNA regions containing the restriction sites
SstI Restriction analysis after PCR- amplification of the DNA regions containing the restriction sites
HincII Restriction analysis after PCR- amplification of the DNA regions containing the restriction sites
PCR and sequence specific primers as previously reported --Lio, D., Balistreri, C.R., Colonna-Romano, G., Motta, M., Fran- ceschi, C., Malaguarnera, M., Candore, G., Caruso, C., 2002. Association between the MHC class I gene HFE polymorphisms and lon
PCR and sequence specific primers as previously reported --Lio, D., Balistreri, C.R., Colonna-Romano, G., Motta, M., Fran- ceschi, C., Malaguarnera, M., Candore, G., Caruso, C., 2002. Association between the MHC class I gene HFE polymorphisms and lon
PCR and sequence specific primers as previously reported --Lio, D., Balistreri, C.R., Colonna-Romano, G., Motta, M., Fran- ceschi, C., Malaguarnera, M., Candore, G., Caruso, C., 2002. Association between the MHC class I gene HFE polymorphisms and lon
PCR products were separated by electrophoresis on a 1.5% agarose gel
Multilocul PCR
using the molecular weight (size: 22 bp) difference between the deleted and non-deleted forms of the 2DS4 gene, via gel electrophoresis on a 2% Metaphor agarose gel. non-deleted were confirmed as by employing a sequence specific oligonucleotide prob
Taqman Universal Master Mix (Applied Biosystems)
ABI 377 automatic sequencer (Applied Biosystems)
loaded on 3% agarose (presence/absence of PCR product)
Msp1 restriction enzyme, loaded on 3% agarose.
BstU restriction enzyme, loaded on 3% agarose.
PCR - loaded on 3% agarose. A fragment of 401 bp, or 417 bp if the 16 bp duplication is present
PCR restriction fragment length polymorphism. The fragments were separated on agarose gel.
Taqman-based allelic discrimination assay
Taqman- based allelic discrimination assay. ABI Prism 7700 (Applied Biosystems)
DNA sequencing using the BigDye Terminator cycle sequencing kit and an ABI Prism 3700 DNA analyzer or by real-timepyrophosphate DNA sequencing using a PSQ 96 system
Taqman Allelic Discrimination method on an Applied Biosystems 7700 Sequence Detector
Polymerase chain reaction sequence specific oligonucleotide (PCR-SSO) reverse dot blot using the Dynal RELI SSO system (Hoffman-La Roche Ltd. and Roche Molecular Systems, Inc., Alameda, CA)
PCR fragment amplified using NcoI restriction enzyme, loaded on 2% agarose
PCR and Hha I restriction enzyme digestion on 5% agarose gel and ethidium bromide staining
restriction fragment length polymorphism (RFLP)
A multilocus PCR
Specific PCR of YTHDF2 (TG)12-27 Polymorphism, RT PCR
Direct sequencing analysis of PCR fragments using BigDyeTerminatorTM protocol on an automated 3100ABI Prism Genetic Analyzer (Applied Biosystem, Foster City, CA)
MwoI restriction enzyme
Allele specific PCR products were detected by electrophoresis on 2% agarose
DYEnamic ET Terminator Cycle Sequencing Premix Kit (Amersham Pharmacia Biotech, Piscataway, NJ, USA) on an ABI Prism 3730xl Genetic Analyser (Applied Biosystems, Foster City, CA, USA)
ABI Prism 7000 Sequence Detection System
PCR amplification of short mtDNA fragments, followed by restriction enzyme analysis (RFLP) and confirmed by HVR-I and HVR-II sequencing
Taqman SNP Genotyping Assays (Applied Biosystems) on an automated_x000D_ platform
ABI Prism 7700 Sequence Detector (Applied Biosystems)
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
In 1st and 2nd Cohort, Two methods: template directed primer extension with fluorescence polarization detection (FP- TDI, AcycloPrime II detection kit, Perkin Elmer, Boston, MA, USA) (Hsu & Kwok, 2003) and SNPstream 48plex (Beckman Coulter, Fullerton
SNaPshot minisequencing reaction, PCR
genomic DNA was analyzed on the Illumina 370 CNV chip
polymerase chain reaction-restriction fragment length polymorphism reaction-restriction fragment length polymorphism (PCR-RFLP)
"VDR genotyping followed protocols described by de la Torre et al. (2008)" doi: 10.1086/525043. Followed the article: _x000D_http://jid.oxfordjournals.org/content/197/3/405/F3.expansion.html, Which led me to believe they used:_x000D_ ABI-PRISM 3100 G
"VDR genotyping followed protocols described by de la Torre et al. (2008)" doi: 10.1086/525043. Followed the article: _x000D_http://jid.oxfordjournals.org/content/197/3/405/F3.expansion.html, Which led me to believe they used:_x000D_ ABI-PRISM 3100 G
"VDR genotyping followed protocols described by de la Torre et al. (2008)" doi: 10.1086/525043. Followed the article: _x000D_http://jid.oxfordjournals.org/content/197/3/405/F3.expansion.html, Which led me to believe they used:_x000D_ ABI-PRISM 3100 G
"VDR genotyping followed protocols described by de la Torre et al. (2008)" doi: 10.1086/525043. Followed the article: _x000D_http://jid.oxfordjournals.org/content/197/3/405/F3.expansion.html, Which led me to believe they used:_x000D_ ABI-PRISM 3100 G
"VDR genotyping followed protocols described by de la Torre et al. (2008)" doi: 10.1086/525043. Followed the article: _x000D_http://jid.oxfordjournals.org/content/197/3/405/F3.expansion.html, Which led me to believe they used:_x000D_ ABI-PRISM 3100 G
genome-wide SNP data selected from the Illumina control database
RFLP
Hot start PCR
SNPlexTM Genotyping System (Applied Biosystems)
PCR-based restriction fragment length polymorphism (RFLP) analysis
ABI Prism 310 genetic analyzer; Applied Biosystems & replicared with low-density DNA microarray based on allele-specific probes
Genomic PCR_x000D_ and BclI-RFLP analysis. DdeI restriction enzyme.
Genomic PCR_x000D_ and BclI-RFLP analysis.
DdeI restriction enzyme.
TaqMan SNP genotyping assays
Must purchase article to view
PSQ HS 96A Pyrosequencer
Sequenced in ABI 3730 DNA analyser (Applied Biosystems)
allele-specific PCR-based KASPar SNP genotyping system (KBiosciences, Hoddesdon, UK) (Cuppen
genotyped on a Sequenom MassArray iPLEX platform
Protocol of BigDye v1.1 kit and an ABI 3130XL sequencing system (Applied Biosystems)
DNA sequencing
Each country by one of the following: Illumina Infinium HD Human660W-Quad BeadChips, Illumina HumanOmniExpress BeadChips, Illumina Infinium HD Human610-Quad BeadChips
Illumina 610 Quad Array
Immunobloting
Sentrix Array Matrices
Affymetrix 500K and 50K Mapping Arrays
Sequenom's chip-based matrix-assisted laser desorption/ionization time-of-flight MS and RT-PCR
Western Blotting
Version 3 Illumina Infinium II HumanHap550 SNP chip array, Illumina 370CNV, Affymetrix GeneChip SNP Array 6.0, Illumina 370CNV, Illumina 550K, Illumina 550K, Illumina Human1M-Duo, Affymetrix SNP Array 6.0, respectively.
Sequenom's chip-based DNA MASSARRAY
Illumina: 370 CNV chip v1.0, Human610-Quad v1.0, Human 1 M v1.0
Meta-analysis
Serological tests
ABI-PRISM 3100 G
P value reported is uncorrected, but association did remain significant after correcting for multiple testing. P value for association with age at death.
P value for association with age at death.
Illumina 300, Illumina 610
Finding confirmed in replication cohort.
Illumina HumanHap BeadChip 317K duo system