MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitin.

Authors: Liu T; Tang H; Lang Y; Liu M; Li X

Abstract: MicroRNAs (miRNAs) may function as oncogenes or tumor suppressors. Here, we show that miR-27a is up-regulated in human gastric adenocarcinoma. Suppression of miR-27a inhibits gastric cancer cell growth. Subsequently, prohibitin is identified as a potential miR-27a target, combining bioinformatics and microarray analysis. EGFP report experiment also confirms that the 3' untranslated region (3'UTR) of prohibitin carries the directly binding site of miR-27a. After knockdown of miR-27a in gastric cancer cells, mRNA level and protein level of prohibitin are both elevated. Down-regulation of prohibitin by miR-27a may explain why suppression of miR-27a can inhibit gastric cancer cell growth, further supporting that miR-27a functions as an oncogene.

Keywords: 3' Untranslated Regions; Adenocarcinoma/*metabolism; Binding Sites; Cell Line, Tumor; Cell Transformation, Neoplastic; Disease Progression; *Gene Expression Regulation, Neoplastic; Green Fluorescent Proteins/metabolism; Humans; MicroRNAs/*genetics/*physiology; Oncogenes; Repressor Proteins/*chemistry; Stomach Neoplasms/*metabolism
Journal: Cancer letters
Volume: 273
Issue: 2
Pages: 233-42
Date: Sept. 16, 2008
PMID: 18789835
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Citation:

Liu T, Tang H, Lang Y, Liu M, Li X (2009) MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitin. Cancer letters 273: 233-42.


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