Authors: Olivieri F; Spazzafumo L; Santini G; Lazzarini R; Albertini MC; Rippo MR; Galeazzi R; Abbatecola AM; Marcheselli F; Monti D; Ostan R; Cevenini E; Antonicelli R; Franceschi C; Procopio AD
Abstract: Circulating microRNAs (miRs) have been investigated as diagnostic/prognostic biomarkers in human diseases. However, little is known about their expression throughout the aging process. Eleven healthy individuals aged 20, 80 and 100years underwent miR plasma profiling. The validation cohort consisted of 111 healthy adults (CTR) aged 20-105years and included 30 centenarians. In addition, 34 patients with cardiovascular disease (CVD) and 15 healthy centenarian offspring (CO) were enrolled. An exploratory factorial analysis grouped the miRs into three main factors: factor 1 primarily higher in 20-year-old subjects, but these differences did not reach statistical significance, factor 2 primarily higher in octogenarians and factor 3 primarily higher in centenarians. MiR-21, the most highly expressed miR of factors 2 and 3, was further validated, confirming the differences in the age groups. MiR-21 expression was higher in the CVD patients and lower in the CO compared to the age-matched CTR. MiR-21 was correlated with C-reactive protein and fibrinogen levels. TGF-beta signaling was the predicted common pathway targeted by miRs of factors 2 and 3. TGF-betaR2 mRNA, a validated miR-21 target, showed the highest expression in the leukocytes from a subset of the octogenarians. Our findings suggest that miR-21 may be a new biomarker of inflammation.
|
Journal: Mechanisms of ageing and development Volume: 133 Issue: 11-12 Pages: 675-85 Date: Oct. 9, 2012 PMID: 23041385 |
Olivieri F, Spazzafumo L, Santini G, Lazzarini R, Albertini MC, Rippo MR, Galeazzi R, Abbatecola AM, Marcheselli F, Monti D, Ostan R, Cevenini E, Antonicelli R, Franceschi C, Procopio AD (2012) Age-related differences in the expression of circulating microRNAs: miR-21 as a new circulating marker of inflammaging. Mechanisms of ageing and development 133: 675-85.
Comment on This Data Unit