Feeding Drosophila a biotin-deficient diet for multiple generations increases stress resistance and lifespan and alters gene expression and histone biotinylation patterns.

Authors: Smith EM; Hoi JT; Eissenberg JC; Shoemaker JD; Neckameyer WS; Ilvarsonn AM; Harshman LG; Schlegel VL; Zempleni J

Abstract: Energy restriction increases stress resistance and lifespan in Drosophila melanogaster and other species. The roles of individual nutrients in stress resistance and longevity are largely unknown. The vitamin biotin is a potential candidate for mediating these effects, given its known roles in stress signaling and gene regulation by epigenetic mechanisms, i.e. biotinylation of histones. Here, we tested the hypothesis that prolonged culture of Drosophila on biotin-deficient (BD) medium increases stress resistance and lifespan. Flies were fed a BD diet for multiple generations; controls were fed a biotin-normal diet. In some experiments, a third group of flies was fed a BD diet for 12 generations and then switched to control diets for 2 generations to eliminate potential effects of short-term biotin deficiency. Flies fed a BD diet exhibited a 30% increase in lifespan. This increase was associated with enhanced resistance to the DNA-damaging agent hydroxyurea and heat stress. Also, fertility increased significantly compared with biotin-normal controls. Biotinylation of histones was barely detectable in biotin-deprived flies, suggesting that epigenetic events might have contributed to effects of biotin deprivation.

Keywords: Animal Feed; Animals; Behavior, Animal/drug effects; Biotin/*deficiency/*pharmacology; Biotinylation; Body Composition/drug effects; Drosophila melanogaster/genetics/*metabolism; Female; Gene Expression Regulation/*drug effects; Histones/*metabolism; Hot Temperature; *Longevity; Male; Stress, Physiological/genetics/*metabolism; Transcription, Genetic/genetics
Journal: The Journal of nutrition
Volume: 137
Issue: 9
Pages: 2006-12
Date: Aug. 22, 2007
PMID: 17709434
Select reference article to upload


Citation:

Smith EM, Hoi JT, Eissenberg JC, Shoemaker JD, Neckameyer WS, Ilvarsonn AM, Harshman LG, Schlegel VL, Zempleni J (2007) Feeding Drosophila a biotin-deficient diet for multiple generations increases stress resistance and lifespan and alters gene expression and histone biotinylation patterns. The Journal of nutrition 137: 2006-12.


Study
Update (Admin) | Auto-Update

Comment on This Data Unit