Authors: Shen L; Hu Y; Cai T; Lin X; Wang D
Abstract: In Caenorhabditis elegans, functional ttx-3, sra-11, ceh-10, and ceh-23 genes are required for the functions of AIY interneuron. Compared to wild-type N2, mutations in ttx-3 and ceh-10 significantly decreased lifespan, whereas mutations in sra-11 and ceh-23 did not obviously influence nematode lifespan. Mutations in ttx-3 and ceh-10 were associated closely with lower pumping rates at adult day 8 and caused a more rapid accumulated intestinal autofluorescence than wild-type N2. Mutations in ceh-10 remarkably affected fertility and egg number in the uterus. The regulation of ttx-3 and ceh-10 on longevity was not temperature-dependent, and ttx-3, and ceh-10 mutants all formed very few dauers at 27 degrees C. The shortened lifespan of the ttx-3 or ceh-10 mutants was completely or largely rescued by expression of TTX-3 or CEH-10 in AIY interneurons. Moreover, the long-lived phenotype of the daf-2 mutant could be suppressed by both the ttx-3 and the ceh-10 mutations. Furthermore, ablation of AIY interneurons shortened the longevity of wild-type and the daf-2 mutant. Therefore, ttx-3 and ceh-10 regulate the longevity through influencing the insulin/IGF signaling pathway in C. elegans.
Keywords: Animals; Caenorhabditis elegans/genetics/*physiology; Caenorhabditis elegans Proteins/genetics/*physiology; Gene Expression; Homeodomain Proteins/genetics/physiology; Insulin/metabolism; Interneurons/*physiology; Intestines/physiology; Longevity/*genetics; Mutation; Neuropeptides/genetics/physiology; Pharynx/physiology; Receptor, Insulin/genetics/physiology; Receptors, G-Protein-Coupled/genetics/physiology|
Journal: Mechanisms of ageing and development Volume: 131 Issue: 11-12 Pages: 732-8 Date: Nov. 9, 2010 PMID: 21055415 |
Shen L, Hu Y, Cai T, Lin X, Wang D (2010) Regulation of longevity by genes required for the functions of AIY interneuron in nematode Caenorhabditis elegans. Mechanisms of ageing and development 131: 732-8.
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