The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms.

Authors: Kaeberlein M; McVey M; Guarente L
Year: 1999
Journal: Genes & development
Abstract: The SIR genes are determinants of life span in yeast mother cells. Here we show that life span regulation by the Sir proteins is independent of their role in nonhomologous end joining. The short life span of a sir3 or sir4 mutant is due to the simultaneous expression of a and alpha mating-type information, which indirectly causes an increase in rDNA recombination and likely increases the production of extrachromosomal rDNA circles. The short life span of a sir2 mutant also reveals a direct failure to repress recombination generated by the Fob1p-mediated replication block in the rDNA. Sir2p is a limiting component in promoting yeast longevity, and increasing the gene dosage extends the life span in wild-type cells. A possible role of the conserved SIR2 in mammalian aging is discussed.
Reference

Integration:

Created on Nov. 5, 2012, 4:46 p.m.
Not linked
Integrated: False

No notes
Species: Budding yeast

Experiments: 0
Interventions:
  • DNL4 deletion
  • YKU70 deletion
  • YKU80 deletion
  • Mating type heterozygosity

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