| 16 | Yeast Dilution DR | ydDR | The reduction in brewer's yeast (e.g. from 6 to 1%) is an effective regimen for DR studies in Drosophila [17921418], which produces longevity effects [19968629]. See also Giannakou et al. 2012 [18241326]. |
| 13 | Solid plate-based dietary restriction | sDR | Solid plate-based DR with (mostly) live or dead E. coli started at day 5 of adulthood increases lifespan by 18-35% and decreases fertility [Greer et al., 2007]. |
| 15 | Liquid media/culture DR | lDR | Restriction of live E. coli in liquid + solid media (antibiotics) started usally at L4/young adulthood increases lifespan by 28% and decreases fertility [Bishop & Guarente, 2007]. |
| 19 | Ad libitum | AL | Medium: Solid
Source of food: Live E. coli |
| 3 | eat-2 mutation | eat-2 | Loss of function alleles of eat-2 (encoding a non-alpha-nicotinic acetylcholine receptor subunit ), increase lifespan by 0-57% while decreasing fertility, are considered as a genetic forms of dietary restriction [9789046].
Lakowski & Hekimi, 1998
Panowski et al ., 2007
Kaeberlein et al ., 2006
Lee et al., 2006
Hansen et al., 2007
Houthoofd et al ., 2002b |
| 9 | Water starvation | WS | Incubation in water represents a form of starvation/extreme dietary restriction, which similar to the reduction of glucose content in the growth medium, promotes lifespan extension [7,39,44,49 in Fabrizio et al., 2010]. |
| 5 | Bacterial dilution | bDR | Dilution of the live bacteria (antibiotics) on liquid media usually started at day 2 of adulthood increase lifespan by 60-70% and decrease fertility at the same time.
Panowski et al., 2007
Houthoofd et al., 2003
Klass, 1977 |
| 17 | Food dilution DR | fdDR | Dilution of the food as described by Giannakou et al., 2008 [18241326]. |
| 14 | Peptone Dilution | PD/DP | Dilution of peptone on solid media with live E. coli. applied at birth extends the lifespan by 33% and increases fertility [Hosono et al., 1989]. |
| 11 | Dietary/bacterial depletion/deprivation | DD/BD | Bacterial deprivation, the complete removal of food (E. coli) on solid media, during early adulthood (day 2) extends lifespan by 42-50% with unknown effect on fertility [17081160;17096674;18457595;18083317; Kaeberlein et al., 2006; Lee et al ., 2006; Steinkraus et al., 2008]. |
| 22 | Resveratrol | RES | Application of DR-mimetic resveratrol on solid live or dead E. coli started at birth extends the lifespan by 6-14% with no effect on fertility [Wood et al., 2004; Viswannathan et al., 2005; Bass et al., 2007]. |
| 20 | Axenic media | Axenic | A liquid defined chemical broth starting at Larval day 4 (L4) increases lifespan by 80-150% and decreases fertility [Houthoofd et al., 2002b; Houthoofd et al., 2003; Houthoofd et al., 2002a]. |
| 21 | Chemical defined liquid media | CDML | A chemical defined liquid birth applied at birth increases lifespan by 80% and decreases fertility [Szewczyk et al., 2006]. |
| 1 | 0.05% Glucose restriction | severe DR | — |
| 2 | 0.5% Glucose restriction | moderate DR | — |
| 4 | Intermittent Fasting | IF | — |
| 6 | Glucose restriction | GR | — |
| 7 | Dietary restriction | DR | — |
| 8 | Amino acid restriction | AAR | — |
| 10 | Genetic DR mimetic | GDRM | — |
| 12 | Minimal Medium | MM | — |
