|Trichostatin A supplementation ||Histone deacetylase inhibitor Trichostatin A (TSA) extends the lifespan of Drosophila melanogaster by promoting the hsp22 gene transcription, and affecting the chromatin morphology at the locus of hsp22 gene along the polytene chromosome .
hsp70 and hsp22 RNA levels are higher in long-lived than in short-lived fly lines. The HDAC inhibitor TSA causes a higher expression of hsp22 and hsp70, and strikingly influences the lifespan in both long and short-lived lines, with variable degrees (up to 25%) . ||Fly ||+25 ||— ||— |
|DATS treatment ||Treatment with 5-10 μM DATS increases lifespan even when treatment is started during young adulthood. DATS increases the lifespan of daf-2 and daf-16 mutants, but not that of eat-2 mutants.
DATS treatment leads to the induction of the skn-1 target gene gst-4 and this induction is dependent on skn-1. DATS effect on lifespan is dependent on skn-1 activity in both intestine and ASI neurons . ||Fly ||— ||— ||— |
|THC treatment ||Tetrahydocurcumin extends the lifespan and reduces oxidative stress in male and female fruit flies. THC extends lifespan of Drosophila and inhibits the oxidative stress response by regulating *FOXO* and *Sir2* . ||Fly ||— ||— ||— |
|(R)-N-(2-heptyl)-N-methylpropargylamine treatment ||Addition of 0.66 ng/fly/day (R)-N-(2-heptyl)-N-methylpropargylamine to a sucrose-based diet resulted in no significant effect on lifespan, but lifespan reduction due to galactose feeding is partially suppressed by supplementation with (R)-deprenyl or (R)-N-(2-heptyl)-N-methylpropargylamine . ||Fly ||— ||— ||— |
|Metformin treatment ||In fruit fly feeding metformin to adult s results in robust AMPK activation and reduces lipid stores, but does not increase lifespan in either males or females. Administration of high concentration are even toxic .
||Fly ||— ||— ||— |
|Minocycline treatment ||Treatment with minocycline (0.87mM) prolongs mean, median and maximum lifespan of wild-type (Oregon strain) of both genders. In females mincocycline extend mean and maximum lifespan by 57 and 78%, respectively. In males minocycline results in a mean and maximum lifespan extension by 114 and 28%, respectively . ||Fly ||+57.1 to +114.3 ||— ||+28.1 to +78.3 |
|Resveratrol supplementation ||Supplementation with resveratrol extends the lifespan , but not in always . ||Fly ||— ||— ||— |
|Black rice extract supplementation ||In fruit fly, 30 mg/ml black rice extract prolonges mean lifespan by 14% which is accompanied with mRNA up-regulation of SOD1, SOD2, CAT and Rpn11 Rpn11 and with downregulation of Mth . ||Fly ||+14 ||— ||— |
|D-chiro-inositol supplementation ||D-chiro-inositol supplementation to the diet extends adult longevity in both male and female animals. 20 microMolar dose of D-chiro-inositol extends median lifespan by 16.7 (p < 0.001) for males and 13% (p < 0.001) for females. Lifespan extension by D-chrio-inositol is accompanied by protection against oxidative and starvation stresses, improvement in health span, and not reduction in fecundity. Nuclear localization of foxo increases in D-chiro-inositol-fed animals .
||Fly ||— ||+13 to +16.7 ||— |
|Pinitol supplementation ||Pinitol (a 3-methoxy analogue of D-chiro-inositol) supplementation to the diet. For both males and females, a 20 microMolar dose of pinitol significantly extends median lifespan by 13% (p < 0.05) and 12.5% (p < 0.05), respectively. Lifespan extension by pinitol is accompanied by protection against oxidative and starvation stresses, improvement in health span, and no reduction in fecundity. Pinitol increases organismal lifespan of both in dietary restriction and ad libitum conditions. Nuclear localization of foxo increases in pinitol-fed animals. Pinitol treatment significantly activates JNK and S6K, but not AKT . ||Fly ||— ||+12.5 to +13 ||— |
|Cynomorium songaricum supplementation ||The yang-tonifying herbal medicine cynomorium songaricum Repr. (CS) supplementation to the diet extends both the mean and the maximum lifespan of adult females, but insignificantly that of males. In females, maximum lifespan (determined by the 90th survival percentile) is increased by up to 11.4% with 10 mg/mL CS and 5.7% with both 20 and 30 mg/mL Cs. Mean lifespan is significantly extended by 15, 18 and 11% upon treatment with 10, 20, and 30 mg/mL CS, respectively (all P <0.001). Increased lifespan by CS is correlated with higher resistance to oxidative stress and starvation and lower lipid hydroxyperoxids levels as well as accompanied by beneficial effects, such as improved mating readiness, increased fecundity, and suppresion of age-related learning impairment in aged animals . ||Fly ||+11 to +18 ||— ||+5.7 to +11.4 |
|Black tea extract supplementation ||Supplementation of the diet with black tea extract extends the lifespan by 10% (from 51 to 56 days) and is associated with higher SOD1 and CAT expression . ||Fly ||+10 ||— ||— |
|Apple polyphenol supplementation ||Supplemention of the diet with apple polyphenol significantly extends mean lifespan by 10% and is accompanied by up-regulation of SOD1, SOD2 and CAT as well as downregulation of MTH in aged animals . ||Fly ||+10 ||— ||— |
|Blueberry extract supplementation ||Supplementation of the diet with 5 mg/mL blueberry extract significantly extends the mean lifespan by 10% and is accompanied by an up-regulation of superoxide dismutase (SOD), catalase (CAT), and Rpn11 and down-regulationg of Methuselah (MTH). Lifespan is only extended in Oregon-R wild-type but not in SOD(n108) or Cat(n1) mutant strains . ||Fly ||+10 ||— ||— |
|Oligomycin treatment ||Oligomycin (a specific inhibitor of complex V) feeding exends lifespan on ad libitum and prevents an increase in longevity under DR (started in the adulthood) in males . ||Fly ||— ||— ||— |
|Restriction of yeast ||Restriction of yeast, the major source of protein in the lfy diet, robustly extends lifespan [15186745; 16000018]. ||Fly ||— ||— ||— |
|GGTI-298 treatment ||Treatment with type 1 geranylgeranyl transferase inhibitor GGTI-298 increases lifespan . ||Fly ||— ||— ||— |
|L744832 treatment ||Farnesyl inhibitor L744832 increases lifespan . ||Fly ||— ||— ||— |
|Simvastin treatment ||Treatment with simvastin significantly increases the mean and maximum lifespan and enhances cardiac function in aging animals by significantly reducing heart arrhythmias and increasing the contraction proportion o the contraction/relaxation cycle . ||Fly ||— ||— ||— |
|Low calorie diet with low-sugar content ||A diet with low-calorie and low-sugar content increase the lifespan, but not resistance to acute oxiditive stress  ||Fly ||— ||— ||— |
|High sugar low protein diet ||A high sugar low protein diet increases the lifespan, but not resistance to acute oxidative stress . ||Fly ||— ||— ||— |
|PDTC treatment ||Treatment of Drosophila imago with PDTC increases median (by 11-13%) and maximum (by 11-14%) lifespan in females and males, respectively . ||Fly ||— ||+11 to +13 ||+11 to +14 |
|Rapamycin treatment ||Treatment of Drosophila imago with rapamycin induces increases of median (by 5-6%) lifespan (p < 0.01) in males and females, respectively and increase of maximum lifespan (by 33%) in females (p < 0.01) .
Low dose of LY294002 (5 microM) slightly increase the median and maximum lifespan . ||Fly ||— ||+5 to +6 ||+33 |
|Wortmannin treatment ||Treatment of Drosophila imago with 0.5 micromolar wortmannin increases median (by 5%) and maximum (by 39%) lifespan in males (p < 0.001), but the lifespan differences in females were statistical insignificant (p > 0.05) .
Low dose of wortmannin (5 microM) slightly increase the median and maximum lifespan . ||Fly ||— ||+5 ||+39 |
|LY294002 treatment ||Treatment of Drosophila imago with 5 micromolar LY294002 increases median (by 14%) and maximum (by 16-22%) lifespan (p<0.001) in females and males, respectively .
Low dose of LY294002 (5 microM) slightly increase the median and maximum lifespan . ||Fly ||— ||+14 ||+16 to +22 |