| aak-2 constitutive active mutation | A constitutive active mutation of aak-2 is sufficient to cause increase stress resistance as well as to significantly extend lifespan. Both increased stress resistance and extended lifespan is reverted in daf-16 knockdown by RNAi [17900900]. | Worm | — | — | — |
| aak-2 overexpression | Transgenic animals with a higher aak-2 gene dose live on average 13% longer with a maximum lifespan extension on up to 25% [15574588]. | Worm | +13 | — | +25 |
| aakg-2 overexpression | Overexpression of aakg-2 extends mean, median, and maximum lifespan by 47, 45, and 35% [22737090]. | Worm | +47 | +45 | +35 |
| abu-11 overexpression | Overexpression of abu-11 extends mean lifespan by 9% to 28% [16256736]. | Worm | +9 to +28 | — | — |
| Activating let-60 mutation | The let-60(n1046gf) activating mutation greatly reduces lifespan of wild-type, weakly suppresses constitutive dauer diapause in daf-2 and age-1 mutants and extends lifespan induced by mutation of daf-2 [16164423]. | Worm | — | — | — |
| age-1 RNAi | RNAi against age-1 extends lifespan by 30% [8700226; 8608934]. age-1 RNAi increases mean and maximum lifespan by 36-46% and 48-50% [12447374]. RNAi against age-1 increases mean lifespan by 83% [18828672]. age-1(mg44) zygotic null mutants have a mean (99%) and maximum (117%) lifespan extension [18828672]. | Worm | +36 to +99 | — | +48 to +117 |
| aqp-1 overexpression | Overexpression of aqp-1::GFP rescues short lifespan of aqp-1 deletion mutants and partially prevented glucose from shortening lifespan. | Worm | — | — | — |
| cbp-1 overxpression | Overexpression of cbp-1 does not significantly affect lifespan [19924292]. | Worm | — | — | — |
| clk-1 overexpression | Overexpression of clk-1 shortens lifespan and is associated with increased mitochondrial activity [10202142]. | Worm | — | — | — |
| clk-1 overexpression | Overexpression of clk-1 shortens lifespan and is associated with increased mitochondrial activity [10202142]. | Worm | — | — | — |
| Coq7 overexpression | Transgenic overexpression of mouse Coq7 reverts the extended lifespan of clk-1 mutants [11511092]. | Worm | — | — | — |
| cst-1 overexpression | Overexpression of cst-1 extends lifespan and delays aging in a daf-16-dependent manner [16751106]. | Worm | — | — | — |
| cup-4 overexpression | cup-4 overexpession reduces oxidative stress resistance and shortens lifespan of wild-type under AL [19783783]. | Worm | — | — | — |
| daf-16 overexpression | Overexpression of wild-type DAF-16 modestly increases lifespan by 20% [11747825]. | Worm | +20 | — | — |
| daf-18 overexpression | Overexpression increases adult lifespan in individual tissues [16153634]. | Worm | — | — | — |
| faah-1 overexpression | faah-1 overexpression reduces eicosapentaenoyl ethanolamide (EPEA), palmitoleyol ethanolamide, linoleyol ethanolamide, as well as arachidonoyl ethanolamide (AEA) levels, delays development, increases thermal stress resistance, and was associated with mean and maximum adult lifespan extension by 19 and 35%, respectively, in presence of abundant food but not under (two different protocols of) DR. Overexpression in pharynx was largely sufficient for this lifespan extension [21562563]. | Worm | +19 | — | +35 |
| hsf-1 overexpression | Overexpression of hsf-1 extends the mean, median, and maximum lifespan by 37, 35, and 29% [22737090]. | Worm | +37 | +35 | +29 |
| hsp-16 overexpression | Overexpression of the hsp-16 loci enhances stress resistance and extends mean lifespan by 11% at 20 degree Celsius. Lifespan extension by hsp-16 overexpression requires daf-16 [12882326]. | Worm | +11 | — | — |
| hsp-6 overexpression | Overexpression of hsp-6 from a muscle-specific promoter extends lifespan mean and maximum lifespan by 43 and 45% relatively to animals expressing GFP from the same promoter [11959102]. | Worm | +43 | — | +45 |
| INS overexpression | Expression of human insulin under an inducible heat shock promoter increases nematode lifespan by 25% and is also able to enhance the lifespan of daf-2 mutants [11274053]. | Worm | +25 | — | — |
| ins-1 overexpression | Increased dosage of ins-1 under its own promoter as well as a heat shock promoter increases lifespan by 25% and is also able to increase the lifespan of daf-2 mutants. Overexpression of ins-1 also causes an increase in dauer formation and can enhance the dauer formation of daf-2 mutants [11274053]. | Worm | +25 | — | — |
| jnk-1 overexpression | Overexpression of jnk-1 increases lifespan by 40% [15767565; 23097426].
JNK-1 overexpression extends the lifespan in a daf-16-dependent manner. JNK-1 overexpression does not extend the lifespan of animals unable to synthesize miRNAs, i.e. pash-1(mj100) [23097426]. | Worm | +40 | — | — |
| lars-2 mutation | A mutation that impairs mitochondrial function was associated with a longer lifespan. Mutation of lrs-2/lars-2(mg312) extends lifespan and is associated with impaired mitochondrial function. The recessive allele mg312 of lars-2 extends lifespan by 200% at 20 degree Celsius and 30% at 25 degree Celsius. Lifespan extension by mg312 was not dependent on daf-16(mgDf47). Homozygous lars-2(mg312) worms had multiple pleotropies like lower rates of growth, pumping and defecation as well as remain the size of early L4 worms and are sterile, with an arrested gonad that exhibited no germ-cell differentiation lars-2 is ubiquitously express, with prominent expression in body-wall muscle and neurons, with a mitochondrial subcellular localisation. Mitochondria of lars-2 are noticeably disorganized, swollen and sometimes fused. lars-2 animals have lower ATP content and oxygen consumption [12447374]. | Worm | +30 to +200 | — | — |
| lin-14 gain-of-function mutation | A gain-of-function mutation in lin-14 decreases lifespan [16373574]. | Worm | — | — | — |
| lin-4 overexpression | Overexpressing lin-4 extends lifespan [16373574]. | Worm | — | — | — |
GenAge
GenDR
