| DNApol-gamma35 overexpression | Overexpression of DNApol-gamma35 (DNA polymerase gamma) in the nervous system results in a decrease in the median lifespan ranging from 39% to 52% [17999718]. | Fly | -39 to -52 | — | — |
| dys knockout | Loss of dys function in the heart leads to an age-dependent disruption of the myofibrillar organization within the myocardium as well as to alterations in cardiac performance. Mesodermal dys knockout results in a morderate maximum lifespan reduction (13%), but not when exclusively targeted to the heart. In contrast, half of the transheteozygous DysExel618/Dyskx43 deficiency flies die at 29 days compared to 63 days in controls. This indicates that a moderate dye loss-of-function in all muscles, but not in just the heart, reduces the normal lifespan [18221418]. | Fly | — | — | -13 |
| dys RNAi | dys RNAi-mediated knockdown in the mesoderm shortens lifespan [18221418]. | Fly | — | — | — |
| puc mutation | Heterozygous loss-of-function mutations in puc (either pucA241.1 or pucE69) significantly extend median and maximum lifespan and increase resistance to oxidative stress. Heterzyogosity for puc only modestly extends lifespan in animals carrying a hypomorphic allele of the JNK kinase hep [14602080].
puc heterzyogotes do not differ signficantly from wild-type for body size, reproductive activity or developmental timing, but exhibit increased resistance to oxidative stress and starvation [14602080]. | Fly | — | — | — |
| Edem1 mutation | The mean lifespan of Edem1 mutants of both male and female is increased by more than 30% [19302370]. | Fly | +30 | — | — |
| DHEA treatment | DHEA treatment increases the lifespan of female fruit flies [Li et al., 2000]. | Fly | — | — | — |
| elav mutation | elav mutation significantly decreases the lifespan. Median lifespan in males is 66% lower [20589912]. | Fly | — | — | — |
| esc mutation | Males heterozygous for the null esc4 or the dominant negative esc9 mutation that are progeny of an out-cross to a O-R wild-type strain have median lifespan that is, respectively, 47% and 60% longer than the O-R control. When derived from an out-cross to a longer-lived C-S wild-type strain, heterozygous esc9 flies have a median lifespan that is 43% longer than the C-S control [20018689]. | Fly | +47 to +60 | — | +34 |
| fh overexpression | Overexpression of fh in the mitochondria of female transgenic animals increases antioxidant capability, resistance to oxidative stress insults, and longevity [18258192]. | Fly | — | — | — |
| Gr63a mutation | Gr63a loss-of-function in female flies leads to 30% extended mean lifespan, increased fat deposition, and enhanced resistance to some (but not all) environmental stresses. Lifespan of males is not extended [20422037]. | Fly | +30 | — | — |
| Gr63a overexpression | Overexpression of Gr63a has modest negative effect on lifespan [20422037]. | Fly | — | — | — |
| GstS1 overexpression | GstS1 overexpression increases the mean lifespan by 33% [18059160]. | Fly | +33 | — | — |
| hebe overexpression | Adult-specific overexpression of hebe increases the lifespan by 5-30% and modulates late-age female fecundity. Female and male mean lifespan is up to 11% and 24% higher [19011900]. | Fly | +5 to +30 | — | — |
| Hsc70-3 overexpression | Overexpression of Hsc70-3 increases average female lifespan by 27% [18059160]. | Fly | +27 | — | — |
| Trichostatin A supplementation | Histone deacetylase inhibitor Trichostatin A (TSA) extends the lifespan of Drosophila melanogaster by promoting the hsp22 gene transcription, and affecting the chromatin morphology at the locus of hsp22 gene along the polytene chromosome [15346199].
hsp70 and hsp22 RNA levels are higher in long-lived than in short-lived fly lines. The HDAC inhibitor TSA causes a higher expression of hsp22 and hsp70, and strikingly influences the lifespan in both long and short-lived lines, with variable degrees (up to 25%) [15695762]. | Fly | +25 | — | — |
| Hsp22-promoter driven reporter overexpression | Hsp22-promoter driven reporter overexpression reduces mean and maximum lifespan [19420297]. | Fly | — | — | — |
| aPKC transposition | Insertion of a P-based vectors in the structural part of aPKC increase male and female lifespan [22661237]. | Fly | — | — | — |
| Gadd45 overexpression | Gadd45 overexpression in the nervous system leads to a significant increase of lifespan without a decrease in fecundity and locomotor activity. The lifespan extension effect is more pronounced in males than in females. Additional maximum lifespan is also extended. The maximum lifespan is increased by 50% and 59% for females and males, respectively. The median lifespan is extended by 46 and 77% for females and males, respectively [22661237]. | Fly | — | — | — |
| Rdh overexpression | Overexpression of Rdh from a doxycycline-inducible promoter results in a 6-17% increase in mean lifespan [12620118].
Rdh is an open reading frame in the first intron of the encore gene [12620118]. | Fly | +6 to +17 | — | — |
| CG3776 overexpression | Overexpression of CG3776 (alias Jhebp29) reduces the mean lifespan, where the reduction in males is slightly higher.
The lifespan of female and male flies with overexpressed CG3776 is reduced by 35% and 42.6%, respectively when compared with Oregon R flies [18275960].
| Fly | -35 to -42.6 | — | — |
| esg transposition | Disruption of esg by insertion of the P{GT1} vector 300 bp downstream of its structural part increases male and female lifespan [22661237]. | Fly | — | — | — |
| CG3776 underexpression | Underexpression of CG3776 (alias Jhebp29) reduces the mean lifespan, where the reduction in males is slightly higher. The lifespan of females and male flies underexpressed CG3776 is reduced by 31.6% and 38.8%, respectively when compared with Oregon R flies [18275960]..
| Fly | -31.6 to -38.8 | — | — |
| insc transposition | insc disruption through an insertion of the P{EPgy2} vector in ts structural part prolongs female lifespan [22661237]. | Fly | — | — | — |
| sgg transposition | Several insertions of P-based vectors in the structural part of sgg are associated with alterations of male and female lifespan [22661237]. | Fly | — | — | — |
| kuk Overexpression | Overexpression of kugelkern in the adult muscle results in a 60% reduction of mean lifespan [18494863]. | Fly | -60 | — | — |
GenAge
GenDR
