Interventions

  • name effect species mean median maximum
    CIT2 overexpression Overexpression of CIT2 has no effect on replicative lifespan [10224252]. Yeast
    HSP104 overexpression Overexpression of HSP104 driven by GAL promoter is insufficient to extend replicative lifespan [9851879]. Overproduction of HSP104 in wild-type cells has no effect on replicative life span, but suppresses the reduced lifespan of Sir2-deficient cells [17908928]. HSP104 overproduction is sufficient to induce thermotolerance [8643570]. Yeast
    RAS1 overxpression No lifespan extension results from overexpression of RAS1 (in SP1) [8034612]. Yeast
    SCP1 overexpression Overexpression of SCP1 leads to elevuated ROS levels and reduces chronological lifespan [15024029]. Yeast
    SGS1 overexpression Overexpression of SGS1 extends the maximum lifespan of cells lacking SRS2, but not the mean lifespan [11861900]. Yeast
    SOD1 overexpression The overexpression of Sods, mitochondrial Sod2 and cytosolic CuZnSod (Sod1), in combination delays the age-dependent reversible inactivation of mitochondrial aconitase, a superoxide-sensitive enzyme, and extends chronological lifespan by 30% [12586694]. Overexpression of SOD1 with CCS1 levuates the level of Cn, Zn-Sod activity and increased chronological lifespan. However overexpression of SOD1 without high cooper or simultonous overexpression of CCS1 shortened both chronological and replicative lifespan [15659212]. Overexpression of SOD1 has no effect on replicative lifespan [10224252]. Yeast
    SOD2 overexpression Combined overexpression of SOD1 and SOD2 extends chronological lifespan by 30% in EG103 strain [12586694]. Yeast
    IME1 transient overexpression Transient overexpression of IME1 resets the replicative lifespan of old cells back to that of young cells [21700873]. Yeast
    NDT80 transient overexpression Transient overexpression of NDT80 rejuvenates old cells [21700873]. Yeast
    ERG2 overexpression Overexpression of ERG2 with the promoter of ERG6 (Perg6-ERG2) extends replicative lifespan and this effect was overlapping with moderate DR, because DR can not extend the lifespan of this mutant [Tang et al., unpublished]. Yeast
    HST2 overexpression HST2 overexpression extends replicative lifespan. 0.5% glucose restriction does not increase lifespan of sir2;fob1;hst2 triple mutants [16051752]. DR increases lifespan of all four sir2;fob1;hstX(X = sirtuin) triple mutants [16741098; 17129213]. Yeast
    CLN3 overexpression Overexpression shortens chronological lifespan together with age-dependent increases in genome instability and apoptosis. While around 80% of wild-type cells are alive almost non CLN3 overexpressers are alive (under condition that avoids adaptive regrowth) [17710147]. Yeast
    FBP1 overexpression Overexpression of FBP1 shortens chronological lifespan [16199065]. Yeast
    CTA1 overexpression CTA1 overexpression partially suppresses the shortened chronological lifespan by ISC1 mutation [21707788]. Yeast
    MAPK1 overexpression Overexpression of human MAPK1 (alias ERK2) confers resistance to heat shock and oxidative stress extends median chronological lifespan by 24% and was statistically non-addative with cyr1-1 mutation [17662940]. Yeast +24
    MXR1 overexpression Overexpression of MXR1 (alias MsrA) slightly increases the replicative lifespan [15141092]. Yeast
    MXR2 overexpression Overexpression of MXR2 (alias MsrB) has no effect on replicative lifespan under normal growth conditions, but under DR conditions extends replicative lifespan by 120% [15141092]. Yeast
    BMH2 overexpression Overexpressing 14-3-3 protein, Bmh2, significantly extends median chronological lifespan by activating stress response [19805817]. Yeast
    OSH3 overexpression Overexpression of OSH3 with the promoter of VAC8 shortens mean replicative lifespan ad promotes vacuolar fusion [Xia et al. unpublished]. Yeast
    OSH7 overexpression Overexpression of OSH7 extends mean replicative lifespan. PERG6-OSH7 does not extend the maximum lifespan significantly [Xia et al., unpublished]. Yeast
    PEP4 overexpression Overexpression of vacuolar aspartyl protease (PEP4) extends chronological lifespan by increasing cytosolic polyamine and S-adenosylmethionine (SAM) levels. Deletion of PEP4 results in both apoptotic and necrotic cell death during chronological aging [21593793]. Yeast
    NNT1 overexpression Overexpression of NNT1 by 5-fold extends mean and maximum replicative lifespan by 18 and 23%, which is approximately of the same magnitude as the lifespan extension obtained from DR.DR in NNT1 overexpression mutant fails to significantly affect the lifespan and only results in extended mean lifespan by 12% and reduced maximum lifespan by 11%. NNT1 overxpression increases rDNA silencing [12736687]. Yeast +18 +23
    Overexpression of truncated TLC1 Overexpression of a truncated allele of TLC1 abrogates telomere silencing [7545955], shortened telomeres and extends replicative lifespan approximately by 20% [9275199]. Deletion of TLC1 might decrease replicative lifespan [Nugent et al., 1996]. Yeast +20
    LAT1 overexpression In contrast, overexpressing LAT1 extends replicative lifespan, and this lifespan extension was not further increased by 0.5% glucose restriction. Similar to DR, replicative lifespan extension by LAT1 overexpression largely requires mitochondrial respiration [17200108]. Overexpressing Lat1 extends lifespan (20% mean lifespan increase) and this lifespan extension is not further increased by DR. Similar to DR, lifespan extension by LAT1 overexpression largely requires mitochondrial respiration indicating mitochondrial metabolism plays an important role in DR. Interestingly, LAT1 overexpression does not require the Sir2 family to extend lifespan. Lat1 is also a limiting longevity factor in non-dividing cells in that overexpressing LAT1 extends cell survival during prolonged culture at stationary phase. Yeast +20
    MPT5 overexpression Overexpression of MPT5 from the ADH promoter extends replicative lifespan by about 20% in W303R [11805047] and by 25% in PSY142 [9150138]. MPT5 overexpression suppresses the temperature phenotype of POP2 mutant [9504907]. Yeast +20 to +25
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    • 25 of 42 interventions
    Interventions are an extension of GenAge and GenDR.