|magu overexpression ||Adult-specific overexpression of magu increases lifespan by 5-30% and modulates late-age fecundity . ||Fly ||+5 to +30 ||— ||— |
|Loco mutation ||Reduced expression of Loco due to hetero-deficient results in a 17-20% longer mean lifespan for both male and females, besides the fact that the homozygous deficiency of loco is lethal. Several of these long-lived mutants are more resistant to stresses such as starvation, oxidation ad heat. Additional mutant have higher Manganese-containing superoxide dismutase (MnSOD) activity, increased fat content an diminished cAMP levels. Loco RGS domain is required for the regulation of longevity as deletion analysis suggest . ||Fly ||+17 to +20 ||— ||— |
|Lnk mutation ||Loss of Lnk function results in increased median (14% in females and 17.5 in males) and maximum lifespan, reduced female fecundity and improves survival under conditions of oxidative stress and starvation. Heterozygousity does not result in any significant differences in lifespan in either males or females. Moreover, lifespan extension in one of the female homozygous mutant is fully rescued by the introduction of a Lnk genomic rescue construct . ||Fly ||— ||-14 to -17.5 ||— |
|LBR overexpression ||Overexpression of Lamin B receptor in the adult muscle and in the abdominal fat body results in a 54% and 46% reduction of mean lifespan, respectively . ||Fly ||— ||— ||— |
|kuk Overexpression ||Overexpression of kugelkern in the adult muscle results in a 60% reduction of mean lifespan . ||Fly ||-60 ||— ||— |
|kermit disruption ||The disruption of kermit (alias dGIPC) function results in premature loss of locomotor activity and reduced mean lifespan . ||— ||— ||— ||— |
|CG3776 underexpression ||Underexpression of CG3776 (alias Jhebp29) reduces the mean lifespan, where the reduction in males is slightly higher. The lifespan of females and male flies underexpressed CG3776 is reduced by 31.6% and 38.8%, respectively when compared with Oregon R flies ..
||Fly ||-31.6 to -38.8 ||— ||— |
|CG3776 overexpression ||Overexpression of CG3776 (alias Jhebp29) reduces the mean lifespan, where the reduction in males is slightly higher.
The lifespan of female and male flies with overexpressed CG3776 is reduced by 35% and 42.6%, respectively when compared with Oregon R flies .
||Fly ||-35 to -42.6 ||— ||— |
|Ilp2 RNAi ||Ilp2 RNA interference results in a 24% to 47% increase in median lifespan . ||Fly ||— ||+24 to +47 ||— |
|Ablation of median neurosecretary cells ||Flies with an ablation of median neurosecretary cells (which eliminates Ilp2 expression) exhibit a significant increase in mean and maximum lifespan over that of control flies and an increase to oxidative stress and starvation. The mutants also exhibit increased storage of lipid and carbohydrate, reduced fecundity, and reduced tolerance of heat and cold . The median and maximum lifespan of females is increased by 33.5% and 40%, respectively. In males the median and maximum lifespan is increased by 10.5% and 27%, respectively . ||Fly ||— ||+10.5 to +33.5 ||+27 to +40 |
|Hsp22-promoter driven reporter overexpression ||Hsp22-promoter driven reporter overexpression reduces mean and maximum lifespan . ||Fly ||— ||— ||— |
|Hsp22 doxycyline-regulated overexpression ||Doxycyline-regulated overexpression of Hsp22 makes animals more sensitive to heat and oxidative stress as well as reduces the mean lifespan by up to 21%, particularly at higher culture temperature .
||— ||-21 ||— ||— |
|Trichostatin A supplementation ||Histone deacetylase inhibitor Trichostatin A (TSA) extends the lifespan of Drosophila melanogaster by promoting the hsp22 gene transcription, and affecting the chromatin morphology at the locus of hsp22 gene along the polytene chromosome .
hsp70 and hsp22 RNA levels are higher in long-lived than in short-lived fly lines. The HDAC inhibitor TSA causes a higher expression of hsp22 and hsp70, and strikingly influences the lifespan in both long and short-lived lines, with variable degrees (up to 25%) . ||Fly ||+25 ||— ||— |
|Hsc70-3 overexpression ||Overexpression of Hsc70-3 increases average female lifespan by 27% . ||Fly ||+27 ||— ||— |
|hebe overexpression ||Adult-specific overexpression of hebe increases the lifespan by 5-30% and modulates late-age female fecundity. Female and male mean lifespan is up to 11% and 24% higher . ||Fly ||+5 to +30 ||— ||— |
|GstS1 overexpression ||GstS1 overexpression increases the mean lifespan by 33% . ||Fly ||+33 ||— ||— |
|Gr63a overexpression ||Overexpression of Gr63a has modest negative effect on lifespan . ||Fly ||— ||— ||— |
|Gr63a mutation ||Gr63a loss-of-function in female flies leads to 30% extended mean lifespan, increased fat deposition, and enhanced resistance to some (but not all) environmental stresses. Lifespan of males is not extended . ||Fly ||+30 ||— ||— |
|fh overexpression ||Overexpression of fh in the mitochondria of female transgenic animals increases antioxidant capability, resistance to oxidative stress insults, and longevity . ||Fly ||— ||— ||— |
|esc mutation ||Males heterozygous for the null esc4 or the dominant negative esc9 mutation that are progeny of an out-cross to a O-R wild-type strain have median lifespan that is, respectively, 47% and 60% longer than the O-R control. When derived from an out-cross to a longer-lived C-S wild-type strain, heterozygous esc9 flies have a median lifespan that is 43% longer than the C-S control . ||Fly ||+47 to +60 ||— ||+34 |
|elav mutation ||elav mutation significantly decreases the lifespan. Median lifespan in males is 66% lower . ||Fly ||— ||— ||— |
|Edem1 mutation ||The mean lifespan of Edem1 mutants of both male and female is increased by more than 30% . ||Fly ||+30 ||— ||— |
|E(z) mutation ||Flies heterozygous for the protein null E(z)63 or the catalytically inactive E(z)731 mutation that are progeny of an out-cross to an Oregon-R (O-R) wild-type strain exhibit a substantially greater median lifespan than the O-R control (71% and 76%, respectively). When derived from an out-cross to a longer-lived Canton-S (C-S) wild-type strain, the median lifespan of E(z)63 heterozygous is 33% longer than the C-S control . ||Fly ||— ||+33 to +76 ||— |
|dys RNAi ||dys RNAi-mediated knockdown in the mesoderm shortens lifespan . ||Fly ||— ||— ||— |
|dys knockout ||Loss of dys function in the heart leads to an age-dependent disruption of the myofibrillar organization within the myocardium as well as to alterations in cardiac performance. Mesodermal dys knockout results in a morderate maximum lifespan reduction (13%), but not when exclusively targeted to the heart. In contrast, half of the transheteozygous DysExel618/Dyskx43 deficiency flies die at 29 days compared to 63 days in controls. This indicates that a moderate dye loss-of-function in all muscles, but not in just the heart, reduces the normal lifespan . ||Fly ||— ||— ||-13 |