|Moderate DR ||Moderate DR is the restriction of glucose concentration from 2% (*ad libitum*) to 0.5%, which extends the mean, median and maximum replicative lifespan by 45 - 52%, 43 - 50% and 50 - 52%, respectively 
Moderate DR increases vacuolar acidity in young cells and prevents the decline of vacuolar acidity in aging cells. DR also suppresses mitochondrial dysfunciton of aged cells (21 divisions) in a V-ATPase-dependent manner .
Constitutively activating PKA signaling by deleting the Ras GTPase-activating protein IRA2 reduces vacuolar acidity and accelerates the development of mitochondrial dysfunction in aging cells and prevents DR-mediated enhancement of vacuolar acidity and suppression of mitochondrial dysfunction .
Lifespan extension by DR is prevented in a strain lacking V-ATPase activity . ||Yeast ||+45.2 to +51.7 ||+42.9 to +50.0 ||+50.0 to +52.0 |
|Methionine restriction ||Restriction of the methionine content in the culture extends mean and maximum lifespan by up to 29 and 16% (1/10 methionine content) . ||Yeast ||+29 ||— ||+16 |
|Methionine restriction ||A diet with reduced methionine content extends lifespan and increases body fat . ||Mouse ||— ||— ||— |
|Restriction of yeast ||Restriction of yeast, the major source of protein in the lfy diet, robustly extends lifespan [15186745; 16000018]. ||Fly ||— ||— ||— |
|Low calorie diet with low-sugar content ||A diet with low-calorie and low-sugar content increase the lifespan, but not resistance to acute oxiditive stress  ||Fly ||— ||— ||— |
|High sugar low protein diet ||A high sugar low protein diet increases the lifespan, but not resistance to acute oxidative stress . ||Fly ||— ||— ||— |
|Dietary restriction by 1% yeast medium ||Dietary restriction by be reducing yeast content in medium to 1% extends the lifespan . ||Fly ||— ||— ||— |
|Intermittent fasting ||Intemittent fasting diet increases survivorship and improves insuli sensitivity of normal males, but fails to affect either parameter in GHRKO mice . ||Mouse ||— ||— ||— |
|30% Dietary restriction ||30% dietary restriction starting at 2 months of age increases overall, average, median and maximal lifespan. Knockout of Ghr failed to respond with lifespan extension to this regimen . ||Mouse ||— ||— ||— |
|Dietary restriction on high-fat diet ||Dietary restriction on a high-fat diet increases both mean and maximum lifespan by 36% compared to the high-fate diet control group . ||Mouse ||+36 ||— ||+36 |
|Dietary restriction on low-fat diet ||DR under a low-fat diet increases mean and maximum lifespan by 20% and 25%, respectively . ||Mouse ||+20 ||— ||+25 |