|wwp-1 RNAi ||RNA interference of wwp-1 decreases median lifespan by 9% in wild-type animals and 24% in daf-2 mutants . Loss of wwp-1 function by RNAi reduces lifespan at 25 degree Celsius, but not 20 degree Celsius. Reduced levels of wwp-1 completely suppress the extended longevity of eat-2 mutants. wwp-1 RNAi does not suppress the extended lifespan of isp-1 mutants and has only minor suppressive effects on lifespan of another mitochondrial mutant, clk-1, and in cyc-1 RNAi treated worms. RNAi depletion of wwp-1 has no effect on long lifespan of daf-2 mutants .
||Worm ||— ||-9 ||— |
|FRE6 deletion ||FRE6 deletion increases mean replicative lifespan by 14% and cancels out the lifespan extending effect of DR . ||Yeast ||+14 ||— ||-2 |
|unc-78 mutation ||Mutation in unc-78 has no effect on lifespan .
unc-78 mutants move slowly . ||Worm ||— ||— ||— |
|hsp-16 overexpression ||Overexpression of the hsp-16 loci enhances stress resistance and extends mean lifespan by 11% at 20 degree Celsius. Lifespan extension by hsp-16 overexpression requires daf-16 . ||Worm ||+11 ||— ||— |
|Replacement of Cebpa by Cebpb ||Replacing the Cebpa gene by Cebpb increases mean lifespan by about 20% . C/ebpalpha(beta/beta) animals consume more food but weight less than controls , and have a slightly elevated body temperature (0.3-0.5 degree Celsius) . ||Mouse ||+20 ||— ||— |
|Ghr knockout ||Ghr knockouts (the so called Laron mice) are dwarfs with significantly extended lifespan by 40-50% . Ghr-/- mice are significantly longer lived as Ghr+/+ or Ghr+/- mice (by 40-50%) in both females and males [10875265; 19370397]. 30% DR fails to affect overall survival, average or median long-lifespan of Growth hormone receptor knockout (GHRKO) mice and increased maximal lifespan only in females. Insulin sensitivity in GHRKO mutants is greater than in wild-type and is not further increased by DR . Intermittent fasting also fails to extend the long lifespan of GHRKO mice .
Lifespan of mice with a deletion in the Ghr gene live almost 5 years . In C57BL/6J this mutation increases life expectancy by 16 to 26% depending on gender  and in mice of mixed genetic background the increases amounted to 36-55% . Serum levels of GH are elevated in mutant mice  and mutants are smaller than wild-type. IGF-1 and IGFBP-3 levels are also reduced in Ghr mutant mice . The age-associated decline in memory retention is delayed in Ghr mutants . ||Mouse ||+16 to +55 ||— ||— |
|phi-44 RNAi ||phi-44 RNAi leads to 46% mean and 50% maximum lifespan extension. Lifespan extension by phi-44 is not suppressed by daf-16.
phi-44 RNAi animals have lower ATP content and oxygene consumption . ||Worm ||+46 ||— ||+50 |
|lars-2 mutation ||A mutation that impairs mitochondrial function was associated with a longer lifespan. Mutation of lrs-2/lars-2(mg312) extends lifespan and is associated with impaired mitochondrial function. The recessive allele mg312 of lars-2 extends lifespan by 200% at 20 degree Celsius and 30% at 25 degree Celsius. Lifespan extension by mg312 was not dependent on daf-16(mgDf47). Homozygous lars-2(mg312) worms had multiple pleotropies like lower rates of growth, pumping and defecation as well as remain the size of early L4 worms and are sterile, with an arrested gonad that exhibited no germ-cell differentiation lars-2 is ubiquitously express, with prominent expression in body-wall muscle and neurons, with a mitochondrial subcellular localisation. Mitochondria of lars-2 are noticeably disorganized, swollen and sometimes fused. lars-2 animals have lower ATP content and oxygen consumption . ||Worm ||+30 to +200 ||— ||— |
|age-1 RNAi ||RNAi against age-1 extends lifespan by 30% [8700226; 8608934]. age-1 RNAi increases mean and maximum lifespan by 36-46% and 48-50% . RNAi against age-1 increases mean lifespan by 83% . age-1(mg44) zygotic null mutants have a mean (99%) and maximum (117%) lifespan extension . ||Worm ||+36 to +99 ||— ||+48 to +117 |
|pex1 mutation ||Mutation of the promoter region of pex1(S4868), results in lower expression levels and increases lifespan in males and females by 16% and 13%, respectively. pex1 mutation increases mean and maximum lifespan and males/females by 27-23/14-19% and 0-15%, respectively . ||Fly ||+14 to +27 ||— ||0 to +15 |