HSPA9 overexpression

Species: Human (Taxid: 9606)
Factor: HSPA9
Manipulation: Gain-of-Function, Overexpression
Effect:

Overexpression of HSPA9 (mortalin) increases the proliferation potential of normal fibroblasts [11959102]. Transfection of normal human fibroblasts with human HSPA9 (or the murine Hspa9) overexpression vectors led to an increase in the number of population doublings the cells could sustain before senescing (increase varying from 32-60%, depending on the exact construct used). Transfected cells retain a youthful morphology longer than the controls cells, and there is an dealy in appearance of senescence associated beta-galactosidase activity [10838077]. Mot-2 overexpressing cells exhibit a reduction in p53 transcriptional activation (as measured by expression from vectors containing either luciferase or beta-glactosidase driven by p53 binding sites) [10838077], which might partially or wholly explain the effects of Mot-2 on proliferative potential. HSPA9 is differentially distributed and/or translated in normal vs. transformed cells [8454632].

References:

11959102: Extended longevity of Caenorhabditis elegans by knocking in extra copies of hsp70F, a homolog of mot-2 (mortalin)/mthsp70/Grp75.

10838077: Inactivation of p53 and life span extension of human diploid fibroblasts by mot-2.

8454632: Identification of a novel member of mouse hsp70 family. Its association with cellular mortal phenotype.

Aging Relevance Analysis/Source:

GenAge

GenDR

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