| HSC80 | — | Deletion of HSC82 has no effect on replicative lifespan, but shortens chronological lifespan [11361336]. | Budding yeast |
| RAD27 | — | Deletion of RAD27 results in signs of premature aging and approximately 60% reduction in mean replicative lifespan [12024027]. | Budding yeast |
| SUN4 | — | Disruption of SUN4 shortens mean (87.5% of normal), but not maximum, replicative lifespan in BKY1-14c [Austriaco, N.R. (1996) âUTH1 and the Genetic Control of Aging in the Yeast, Saccharomyces cerevisiae.â Ph.D. Thesis, Massachusetts Institute of Technology; 8810036]
SUN4 mutation causes failure of daughter cells to completely detach and results in a multi-budded morphology [10683261]. | Budding yeast |
| YBR225W | — | Deletion of YBR225W decreases replicative lifespan by 40% in the alpha strain [19030232]. | Budding yeast |
| RRG1 | — | Deletion of RRG1 decreases replicative lifespan by 50% in the alpha strain [19030232]. | Budding yeast |
| YDR089W | — | Deletion of YDR089W decreases replicative lifespan by 30% in the alpha strain [19030232]. | Budding yeast |
| YDR132C | — | Deletion of YDR132C decreases replicative lifespan by 35% in the alpha strain [19030232]. | Budding yeast |
| YDR262W | — | Deletion of YDR262W decreases replicative lifespan by 30% in the alpha strain [19030232]. | Budding yeast |
| YGR071C | — | Deletion of YGR071C decreases replicative lifespan by 60% in the alpha strain [19030232]. | Budding yeast |
| ANS1 | — | Deletion of ANS1 decreases replicative lifespan by 25% in the alpha strain [19030232]. | Budding yeast |
| YKL069W | — | Deletion of YKL069W increases sensitivity to oxidative stress and decreases replicative lifespan [19049972]. | Budding yeast |
| YLR460C | — | Deletion of YLR460C decreases replicative lifespan by 30% in the alpha strain [19030232]. | Budding yeast |
| YMR166C | — | Deletion of YMR166C decreases replicative lifespan by 20% in the alpha strain [18340043]. | Budding yeast |
| YNR066C | — | Deletion of YNR066C decrease replicative lifespan by 50% in the alpha strain [19030232]. | Budding yeast |
| YPR011C | — | Deletion of YPR011V decreases replicative lifespan by 20% in the a strain [18340043]. | Budding yeast |
| ESA1 | — | esa1-531 mutant has an even shorter chronological lifespan than PKA1 deletion mutant in both 2% glucose (ad libitum) and water (extreme DR) at 30 degree Celsius, a semipermissive temperature. At the permissive temperature (25 degree Celsius) there is little difference [19303850]. | Budding yeast |
| CYT2 | — | Mutation of CYT2 decreases replicative lifespan by 25% in the alpha strain [18340043; 19030232].
| Budding yeast |
| MPT5 | — | Overexpression of MPT5 from the ADH promoter extends replicative lifespan by about 20% in W303R [11805047] and by 25% in PSY142 [9150138], whereas the deletion of MPT5 shortens lifespan by about 50% [9150138; 7859289]. MPT5 deletion decreases average chronological lifespan by 50%, which is rescued to the wild-type level by PKC1 overexpression [17172436].
MPT5 mutants are temperature sensitive [7845352], hypersensitive to mating pheromone [9154842], and null mutants exhibit increased silencing at telomeres and decreased rDNA silencing [9584615]. Deletion of MPT5 is synthetical lethal with mutation of either SWI4, SWI6, or CCR4 in an ssd1-d background [11805047]. MPT5 overexpression suppresses the temperature phenotype of POP2 mutant [9504907]. MPT5 is required for relocalization of the SIR complex to the nucleolus in sir4-42 strain [7859289]. | Budding yeast |
| YIA6 | — | Deletion of YIA6 decreases replicative lifespan by 30% in the a strain [18340043]. | Budding yeast |
| YOL092W | — | Deletion of YOL092W decreases mean and maximum replicative lifespan by 36 and 21%, respectively. Lifespan of YOL092Y deletion mutants is extended by 0.5% glucose restriction [22912585]. | Budding yeast |
| TLC1 | — | Overexpression of a truncated allele of TLC1 abrogates telomere silencing [7545955], shortened telomeres and extends replicative lifespan approximately by 20% [9275199]. Deletion of TLC1 might decrease replicative lifespan [Nugent et al., 1996].
| Budding yeast |
| YMR018W | — | Deletion of YMR018W increases replicative lifespan by 35% in the alpha strain [18340043]. | Budding yeast |
| LAT1 | — | LAT1 is suggested to play a role in lifespan extension of DR. Deleting LAT1 abolishes replicative lifespan extension induced by 0.5% and 0.05% glucose restriction. In contrast, overexpressing Lat1 extends replicative lifespan, and this lifespan extension was not further increased by 0.5% glucose restriction. Similar to DR, replicative lifespan extension by LAT1 overexpression largely requires mitochondrial respiration [17200108]. Overexpressing LAT1 extends lifespan (20% mean lifespan increase) and this lifespan extension is not further increased by DR. Similar to DR, lifespan extension by Lat1 overexpression largely requires mitochondrial respiration indicating mitochondrial metabolism plays an important role in DR. Interestingly, LAT1 overexpression does not require the Sir2 family to extend lifespan. Lat1 is also a limiting longevity factor in non-dividing cells in that overexpressing LAT1 extends cell survival during prolonged culture at stationary phase. | Budding yeast |
| ISC1 | Inositol phosphoSphingolipid phospholipase C 1 | ISC1 deletion decreases chronological lifespan [21707788; 21707788]. CTA1
overexpression partially suppresses the shortened lifespan by ISC1. Deletion of SIT4 totally rescues the short lifespan of ISC1 mutants [21707788]. HOG1 deletion partially suppresses the premature aging phenotype and short lifespan of ISC1 deletion [22445853]. | Budding yeast |
| RIF1 | RAP1-Interacting Factor 1 | Deletion of RIF1 decrease replicative lifespan by 40% [9275199]. RIF1 deletion increases telomere silencing and length [8319907; 1577274], and therefore likely recruits SIR2 from rDNA to the telomeres which result in lifespan shortening. The sir4-42 allele suppresses the short lifespan of a RIF1 mutant [9275199]. | Budding yeast |
