|daf-16 ||Abnormal DAuer Formation DAF-16, fork head-related transcription factor (daf-16) ||Mutations in daf-16 suppresses life-extension caused by mutations in daf-2 . daf-16 is required for lifespan extension by mutation of daf-2 or age-1 . RNAi against daf-16 decreases lifespan of wild-type, daf-2 or glp-1 mutants [22509016; 16530050]. Loss of function alleles of daf-16 shorten lifespan, but some alleles have lifespan equal to wild-type . daf-16 mutation significantly reduces lifespan under AL (-20%), but does not prevent lifespan extension by sDR. In another experiment daf-16 mutation totally suppresses lifespan extension by sDR . sDR does not stimulate DAF-16 translocation to the nucleus, but daf-16 mutation cancels out the ability of sDR to extend lifespan and to delay the decline in locomotor activity . DR by bacterial dilution extends lifespan of daf-16 mutants . daf-16 mutation decreases lifespan under AL, but fails to prevent bDR to further extend lifespan . IF-induced lifespan-extension by either 24h/48h/72h per 4 days is significantly diminished in null mutants of daf-16. All these regimens extend lifespan of daf-16 to a lesser extent than that of wild-type. daf-16 partially mediates IF-induced longevity . Glucose or glycerol does not shorten lifespan of daf-16 mutants . daf-16 mutation cancels out the lifespan extension effect of sDR and PD, regardless of the concentration of bacteria or peptones. bDR significantly extends lifespan of daf-16 mutants, but to a lesser extent than that of wild-type. eat-2 mutation extends the lifespan of daf-16 mutants to the same extent than that of wild-type. Resveratrol extends lifespan of daf-16 mutants . daf-16 RNAi completely blocks the lifespan extension by daf-2 mutation, but only partially by bDR. daf-16 RNAi attenuates protection against oxidative stress by bDR. daf-16 expression is induced by bDR . Knockdown of daf-16 decreases mean and maximum lifespan by 50% and 54%, respectively . DAF-16 reduces expression of rsks-1 and daf-15 [15253933; 22560223]. daf-16(mgDf47) decreases mean (18-37%) and maximum (29%) lifespan . Overexpression of wild-type DAF-16 modestly increases lifespan by 20% , while overexpression of constitutive nuclear forms of DAF-16 increases lifespan only slightly . daf-16(mu86) mutation decreases mean (44%) and maximum (18%) lifespan . daf-16(mgDf47) decreases mean (18-37%) and maximum (29%) lifespan . daf-16 mutants are dauer defective  and completely suppress all the phenotypes of daf-2 and age-1 mutations, including lifespan extension, dauer arrest, reduced fertility, and viability defects [8247153; 7789761; 9504918; 7789761]. Mutations in daf-16 also suppress lifespan extension of animals that have a germ line ablation . Sex-specific lifespan potential requires daf-16 .
daf-16 mutation suppresses enhanced UV resistance as well as increase longevity of daf-2, daf-23, spe-26, and clk-1 mutants. Mutation in daf-16 does not alter the reduced fertility in spe-26. daf-16 mutants are more fertile than wild-type . ||Nematode |