|symbol ||name ||observation ||species |
|Sirt1 ||sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae) ||Whole-body deletion of Sirt1 in the adulthood results in mice which are seemingly normal in every way. When mice were given low doses of resveratrol after Sirt1 was disabled, there were no discernible improvement in mitochondrial function or any parameter, while mice with normal Sirt1 function given reservatrol showed dramatic increases in energy, mitochondrial biogenesis and function, AMPK activation and increased NAD+ levels in skeletal muscle. When mice lacking Sirt1 were given low doses of reserveratrol, AMPK was unaffected. When doses were significantly increased in these mice, AMPK was activated in a SIRT1-indepent manner, but still no benefit to mitochondrial function resulted . Sirt1 overexpression mimicks the effect on reservatrol on mitochondrial function, but failed to extend lifespan .
SIRT1 knock-out mouse embryonic fibroblasts (MEFs) have a significant greater replicative capacity in culture. p19ARF levels are significantly reduced in SIRT1 knock-out MEFs .
Sirt1-null mice do not exhibit lifespan extension upon Dietary Restriction .
Sirt1 is required for high-magnitude circadian transcription of several core clock genes. It deacetylates Per2, Arntl and histones of clock-controlled genes .
SIRT1 directly  and indirectly  prevents telomere shortening. ||House mouse |
|daf-16 ||Abnormal DAuer Formation DAF-16, fork head-related transcription factor (daf-16) ||Mutations in daf-16 suppresses life-extension caused by mutations in daf-2 . daf-16 is required for lifespan extension by mutation of daf-2 or age-1 . RNAi against daf-16 decreases lifespan of wild-type, daf-2 or glp-1 mutants [22509016; 16530050]. Loss of function alleles of daf-16 shorten lifespan, but some alleles have lifespan equal to wild-type . daf-16 mutation significantly reduces lifespan under AL (-20%), but does not prevent lifespan extension by sDR. In another experiment daf-16 mutation totally suppresses lifespan extension by sDR . sDR does not stimulate DAF-16 translocation to the nucleus, but daf-16 mutation cancels out the ability of sDR to extend lifespan and to delay the decline in locomotor activity . DR by bacterial dilution extends lifespan of daf-16 mutants . daf-16 mutation decreases lifespan under AL, but fails to prevent bDR to further extend lifespan . IF-induced lifespan-extension by either 24h/48h/72h per 4 days is significantly diminished in null mutants of daf-16. All these regimens extend lifespan of daf-16 to a lesser extent than that of wild-type. daf-16 partially mediates IF-induced longevity . Glucose or glycerol does not shorten lifespan of daf-16 mutants . daf-16 mutation cancels out the lifespan extension effect of sDR and PD, regardless of the concentration of bacteria or peptones. bDR significantly extends lifespan of daf-16 mutants, but to a lesser extent than that of wild-type. eat-2 mutation extends the lifespan of daf-16 mutants to the same extent than that of wild-type. Resveratrol extends lifespan of daf-16 mutants . daf-16 RNAi completely blocks the lifespan extension by daf-2 mutation, but only partially by bDR. daf-16 RNAi attenuates protection against oxidative stress by bDR. daf-16 expression is induced by bDR . Knockdown of daf-16 decreases mean and maximum lifespan by 50% and 54%, respectively . DAF-16 reduces expression of rsks-1 and daf-15 [15253933; 22560223]. daf-16(mgDf47) decreases mean (18-37%) and maximum (29%) lifespan . Overexpression of wild-type DAF-16 modestly increases lifespan by 20% , while overexpression of constitutive nuclear forms of DAF-16 increases lifespan only slightly . daf-16(mu86) mutation decreases mean (44%) and maximum (18%) lifespan . daf-16(mgDf47) decreases mean (18-37%) and maximum (29%) lifespan . daf-16 mutants are dauer defective  and completely suppress all the phenotypes of daf-2 and age-1 mutations, including lifespan extension, dauer arrest, reduced fertility, and viability defects [8247153; 7789761; 9504918; 7789761]. Mutations in daf-16 also suppress lifespan extension of animals that have a germ line ablation . Sex-specific lifespan potential requires daf-16 .
daf-16 mutation suppresses enhanced UV resistance as well as increase longevity of daf-2, daf-23, spe-26, and clk-1 mutants. Mutation in daf-16 does not alter the reduced fertility in spe-26. daf-16 mutants are more fertile than wild-type . ||Nematode |