Name: abnormal DAuer Formation 2
Alias: abnormal DAuer Formation DAF-2
Entrez gene ID: 175410
Ensembl gene ID: Y55D5A.5
Species: Worm (Taxid: 6239)
An insulin receptor-like protein which regulates metabolism, controls longevity and prevents developmental arrest at the dauer stage. Binding of INS family members may either stimulate, or antagonise, association of the receptor with downstream mediators such as pdk-1 and age-1. Required for the response to environmental stimuli such as food, pheromone, and temperature. Role in immune function and pathogen resistance. [UniProt]
daf-2 mutants live more than twice as long as controls. daf-2(sa189) mutation extends mean and maximum lifespan by 133 and 129%, respectively, when shifted to 20 degree Celsius. The daf-2(e1370) mutation extends mean and maximum lifespan by 32 and 119%, respectively, when shifted to 25 degree Celsius and by 110 and 145%, respectively, at 20 degree Celsius. daf-2(sa189) mutation extends mean lifespan by 67% as well as maximum lifespan . This lifespan extension requires the activity of daf-16 . The lifespan extension of daf-2(e1370) mutants is cancelled out by daf-16(m26) mutation. daf-2 mutants still exhibit a long lifespan after ablation of the gonad and germ cells. . daf-2(e1370) increases mean (95-118%) and maximum (165%) lifespan . RNAi against daf-2 extends mean and maximum lifespan by 47 and 65% . daf-2 mutation extends lifespan of wild-type and eat-2 mutants . Long lifespan of daf-2 insulin receptor mutation is further extended by sDR. However, daf-2 mutation is not a null mutation, therefore it is still possible that part of sDR-induced increase in lifespan might depend on insulin receptor pathway . DR by bacterial dilution extends lifespan of daf-2 mutants . IF does not markedly extend lifespan of daf-2 mutants . 2% glucose reduce fractions of animals that become dauers at 22.5 degree Celsius in daf-2 mutants. Glucose almost completely suppresses lifespan extension of daf-2 ligand binding domain and tyrosine kinase mutants back to wild-type levels . daf-2 mutation increases average lifespan by 157%. Under AL daf-2 mutation increases lifespan by 30%. bDR increases lifespan by 65%. daf-2 mutation further increases lifespan under bDR by 40%. Resistance to oxidative stress is reduced daf-2 mutation . daf-2 RNAi increases mean lifesapn by 89% . daf-2(m577) mutation increases mean and maximum lifespan by 33 and 29%, respectively, while daf-2(e1370) mutation increases mean and maximum lifespan by 101 and 181%, respectively . DR from eat-2(ad465) mutation has an addative effect on lifespan of daf-2(e1370) adults, but not on lifespan of daf-2(e1368) adults . Mutation in daf-2 in combination with mutation of daf-12 results in nearly 300% increase in lifespan . daf-2 mutants are dauer constitutive  and exhibit reduced brood size [9504918; 9725835]. daf-2 mutants synergize with germ line ablation for lifespan extension  and also exhibit synergy with clk-1 mutation for lifespan prolongation . All the phenotypes of daf-2 mutants are suppressed by mutation of daf-16 [8247153; 8601482; 7789761; 9725835; 9504918]. Mutation of daf-2 increases expression of sod-3 . daf-2(e1370) increases mean lifespan by 146% .
Reducing expression of daf-2 in the adult stage alone extends lifespan .
Assays: Organismal Lifespan
Mean: +32 to +146
Maximum: +119 to +165
Aging Relevance Analysis/Source:
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