Authors: Gorbunova V; Seluanov A
Abstract: Mutations in the clk-1 gene of Caenorhabditis elegans extend worm life span and slow down a variety of physiological processes. Here we report that C. elegans CLK-1 as well as its mouse homologue have DNA binding activity that is specific to the O(L) region of mitochondrial DNA. DNA binding activity of CLK-1 is inhibited by ADP, and is altered by mutations that extend nematode life span. Our results suggest that, in addition to its enzymatic function in ubiquinone biosynthesis, CLK-1 is involved in the regulation of mtDNA replication or transcription.Keywords: Adenosine Diphosphate/pharmacology; Animals; Base Sequence; Binding Sites/genetics; Caenorhabditis elegans/genetics/physiology; Caenorhabditis elegans Proteins/chemistry/genetics/*metabolism; DNA, Helminth/chemistry/genetics/*metabolism; DNA, Mitochondrial/chemistry/genetics/*metabolism; DNA-Binding Proteins/chemistry/genetics/metabolism; Genes, Helminth; Helminth Proteins/chemistry/genetics/*metabolism; Longevity/genetics/physiology; Membrane Proteins/chemistry/genetics/metabolism; Mice; Mitochondrial Proteins; Mutation; Nucleic Acid Conformation; Recombinant Proteins/chemistry/genetics/metabolism
Journal: FEBS letters
Date: April 18, 2002
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Gorbunova V, Seluanov A (2002) CLK-1 protein has DNA binding activity specific to O(L) region of mitochondrial DNA. FEBS letters 516: 279-84.