C. elegans 14-3-3 proteins regulate life span and interact with SIR-2.1 and DAF-16/FOXO.

Authors: Wang Y; Oh SW; Deplancke B; Luo J; Walhout AJ; Tissenbaum HA

Abstract: 14-3-3 proteins are evolutionarily conserved and ubiquitous proteins that function in a wide variety of biological processes. Here we define a new role for C. elegans 14-3-3 proteins in life span regulation. We identify two C. elegans 14-3-3 proteins as interacting proteins of a major life span regulator, the C. elegans SIR2 ortholog, SIR-2.1. Similar to sir-2.1, we find that overexpression of either 14-3-3 protein (PAR-5 or FTT-2) extends life span and that this is dependent on DAF-16, a forkhead transcription factor (FOXO), another important life span regulator in the insulin/IGF-1 signaling pathway. Furthermore, we show that both 14-3-3 proteins are co-expressed with DAF-16 and SIR-2.1 in the tissues critical for life span regulation. Finally, we show that DAF-16/FOXO also physically interacts with the 14-3-3 proteins. These results suggest that C. elegans 14-3-3 proteins can regulate longevity by cooperating with both SIR-2.1 and DAF-16/FOXO.

Keywords: 14-3-3 Proteins/*physiology; Animals; Caenorhabditis elegans/*physiology; Caenorhabditis elegans Proteins/*metabolism/*physiology; Green Fluorescent Proteins/metabolism; Immunoblotting; Longevity; Models, Animal; Organisms, Genetically Modified; RNA Interference; Sirtuins/*metabolism; Transcription Factors/*metabolism
Journal: Mechanisms of ageing and development
Volume: 127
Issue: 9
Pages: 741-7
Date: July 25, 2006
PMID: 16860373
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Wang Y, Oh SW, Deplancke B, Luo J, Walhout AJ, Tissenbaum HA (2006) C. elegans 14-3-3 proteins regulate life span and interact with SIR-2.1 and DAF-16/FOXO. Mechanisms of ageing and development 127: 741-7.

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