SOD2 functions downstream of Sch9 to extend longevity in yeast.

Authors: Fabrizio P; Liou LL; Moy VN; Diaspro A; Valentine JS; Gralla EB; Longo VD

Abstract: Signal transduction pathways inactivated during periods of starvation are implicated in the regulation of longevity in organisms ranging from yeast to mammals, but the mechanisms responsible for life-span extension are poorly understood. Chronological life-span extension in S. cerevisiae cyr1 and sch9 mutants is mediated by the stress-resistance proteins Msn2/Msn4 and Rim15. Here we show that mitochondrial superoxide dismutase (Sod2) is required for survival extension in yeast. Deletion of SOD2 abolishes life-span extension in sch9Delta mutants and decreases survival in cyr1:mTn mutants. The overexpression of Sods--mitochondrial Sod2 and cytosolic CuZnSod (Sod1)--delays the age-dependent reversible inactivation of mitochondrial aconitase, a superoxide-sensitive enzyme, and extends survival by 30%. Deletion of the RAS2 gene, which functions upstream of CYR1, also doubles the mean life span by a mechanism that requires Msn2/4 and Sod2. These findings link mutations that extend chronological life span in S. cerevisiae to superoxide dismutases and suggest that the induction of other stress-resistance genes regulated by Msn2/4 and Rim15 is required for maximum longevity extension.

Keywords: Aconitate Hydratase/metabolism; DNA-Binding Proteins/metabolism; *Fungal Proteins; Mutation; Protein Kinases/*metabolism; Saccharomyces cerevisiae/enzymology/growth & development/*metabolism; *Saccharomyces cerevisiae Proteins; Superoxide Dismutase/*metabolism; Transcription Factors/metabolism; ras Proteins/genetics/metabolism
Journal: Genetics
Volume: 163
Issue: 1
Pages: 35-46
Date: Feb. 15, 2003
PMID: 12586694
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Fabrizio P, Liou LL, Moy VN, Diaspro A, Valentine JS, Gralla EB, Longo VD (2003) SOD2 functions downstream of Sch9 to extend longevity in yeast. Genetics 163: 35-46.

Study Lifespan Factors:
  • SOD2 SuperOxide Dismutase 2

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