[DNA degradation during standard alkaline of thermal denaturation].

Authors: Drozhdeniuk AP; Sulimova GE; Vaniushin BF

Abstract: Essential degradation 8 DNA (up to 10 per cent) with liberation of acid-soluble fragments takes place on the standard alkaline (0,01 M sodium phosphate, pH 12, 60 degrees, 15 min) or thermal (0.06 M sodium phosphate buffer, pH 6.8, 102 degrees C, 15 min) denaturation. This degradation is more or less selective: fraction of low molecular weight fragments, isolated by hydroxyapatite cromatography and eluted by 0.06 M sodium phosphate buffer, pH 6.8 is rich in adenine and thymine and contains about 2 times less 5-methylcytosine than the total wheat germ DNA. The degree of degradation of DNA on thermal denaturation is higher than on alkaline degradation. Therefore while studying reassociation of various DNA, one and the same standard method of DNA denaturation should be used. Besides, both the level of DNA degradation and the nature of the resulting products (fragments) should be taken into account.

Keywords: Alkalies; Chemical Phenomena; Chemistry; *DNA; Hot Temperature; *Nucleic Acid Denaturation
Journal: Biokhimiia (Moscow, Russia)
Volume: 41
Issue: 7
Pages: 1250-5
Date: Jan. 1, 1976
PMID: 999984
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Citation:

Drozhdeniuk AP, Sulimova GE, Vaniushin BF (1976) [DNA degradation during standard alkaline of thermal denaturation]. Biokhimiia (Moscow, Russia) 41: 1250-5.



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