A deuterohemin peptide extends lifespan and increases stress resistance in Caenorhabditis elegans.

Authors: Guan S; Li P; Luo J; Li Y; Huang L; Wang G; Zhu L; Fan H; Li W; Wang L

Abstract: This group has invented a novel deuterohemin containing peptide deuterohemin-AlaHisThrValGluLys (DhHP-6), which has various biological activities including protection of murine ischemia reperfusion injury, improving cell survival and preventing apoptosis. It was hypothesized that DhHP-6 is beneficial on the lifespan of Caenorhabditis elegans (C. elegans) and increases their resistance to heat and oxidative stress. C. elegans were treated with different concentrations of DhHP-6. Survival time and sensitivity to heat and paraquat were investigated. The data demonstrated that the mean survival time of C. elegans was significantly increased (p < 0.05) in the DhHP-6 treated group compared with the control group. The maximum lifespan was not affected by DhHP-6 treatment. DhHP-6 improved the survival rate of C. elegans in the acute heat stress (35 degrees C) and rescued the C. elegans' sensitivity to paraquat in acute oxidative stress. Superoxide dismutase 3 (SOD-3) protein was up-regulated by DhHP-6 treatment. It was further demonstrated that stress resistance genes such as hsp-16.1, hsp-16.49 and sir-2.1 were regulated by DhHP-6. DAF-16 and SIR-2.1 genes are essential for the beneficial effect of DhHP-6. Therefore, the investigation into the beneficial effect of DhHP-6 on C. elegans' lifespan has the potential to develop novel drugs to prevent ageing.

Keywords: Aging/*drug effects/genetics; Animals; Animals, Genetically Modified; Caenorhabditis elegans/*drug effects/genetics/physiology; Caenorhabditis elegans Proteins/biosynthesis/genetics/physiology; Drug Evaluation, Preclinical; Gene Expression Profiling; Gene Knockdown Techniques; Genes, Helminth; Hemin/*analogs & derivatives/pharmacology; Hot Temperature; Longevity/drug effects/genetics; Oligopeptides/*pharmacology; Oxidative Stress/*drug effects; Paraquat/toxicity; Sirtuins/biosynthesis/deficiency/genetics/physiology; Stress, Physiological/*drug effects; Succinate Dehydrogenase/deficiency/physiology; Transcription Factors/deficiency/genetics/physiology
Journal: Free radical research
Volume: 44
Issue: 7
Pages: 813-20
Date: June 10, 2010
PMID: 20528576
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Citation:

Guan S, Li P, Luo J, Li Y, Huang L, Wang G, Zhu L, Fan H, Li W, Wang L (2010) A deuterohemin peptide extends lifespan and increases stress resistance in Caenorhabditis elegans. Free radical research 44: 813-20.


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