Authors: Ogburn CE; Carlberg K; Ottinger MA; Holmes DJ; Martin GM; Austad SN
Abstract: Previous studies indicated that renal tubular epithelial cells from some long-lived avian species exhibit robust and/or unique protective mechanisms against oxidative stress relative to murine cells. Here we extend these studies to investigate the response of primary embryonic fibroblast-like cells to oxidative challenge in long- and short-lived avian species (budgerigar, Melopsittacus undulatus, longevity up to 20 years, vs Japanese quail, Coturnix coturnix japonica, longevity up to 5 years) and short- and long-lived mammalian species (house mouse, Mus musculus, longevity up to 4 years vs humans, Homo sapiens, longevity up to 122 years). Under the conditions of our assay, the oxidative-damage resistance phenotype appears to be associated with exceptional longevity in avian species, but not in mammals. Furthermore, the extreme oxidative damage resistance phenotype observed in a long-lived bird requires active gene transcription and translation, suggesting that specific gene products may have evolved in long-lived birds to facilitate resistance to oxidative stress.
Keywords: Aging/genetics/metabolism; Amanitins/pharmacology; Animals; Biological Evolution; Birds/*genetics/*metabolism; Cells, Cultured; Coturnix/genetics/metabolism; Cycloheximide/pharmacology; Dactinomycin/pharmacology; Embryo, Mammalian/cytology/metabolism; Embryo, Nonmammalian; Fibroblasts/metabolism; *Gene Expression/drug effects; Humans; Hydrogen Peroxide/toxicity; Longevity/*genetics/*physiology; Mice; *Oxidative Stress; Parrots/genetics/metabolism; Phenotype; Species Specificity; Superoxides/toxicity|
Journal: The journals of gerontology. Series A, Biological sciences and medical sciences Volume: 56 Issue: 11 Pages: B468-74 Date: Oct. 30, 2001 PMID: 11682567 |
Ogburn CE, Carlberg K, Ottinger MA, Holmes DJ, Martin GM, Austad SN (2001) Exceptional cellular resistance to oxidative damage in long-lived birds requires active gene expression. The journals of gerontology. Series A, Biological sciences and medical sciences 56: B468-74.
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