Authors: Rao RP; Yuan C; Allegood JC; Rawat SS; Edwards MB; Wang X; Merrill AH Jr; Acharya U; Acharya JK
Abstract: Ceramide transfer protein (CERT) transfers ceramide from the endoplasmic reticulum to the Golgi complex, a process critical in synthesis and maintenance of normal levels of sphingolipids in mammalian cells. However, how its function is integrated into development and physiology of the animal is less clear. Here, we report the in vivo consequences of loss of functional CERT protein. We generated Drosophila melanogaster mutant flies lacking a functional CERT (Dcert) protein using chemical mutagenesis and a Western blot-based genetic screen. The mutant flies die early between days 10 and 30, whereas controls lived between 75 and 90 days. They display >70% decrease in ceramide phosphoethanolamine (the sphingomyelin analog in Drosophila) and ceramide. These changes resulted in increased plasma membrane fluidity that renders them susceptible to reactive oxygen species and results in enhanced oxidative damage to cellular proteins. Consequently, the flies showed reduced thermal tolerance that was exacerbated with aging and metabolic compromise such as decreasing ATP and increasing glucose levels, reminiscent of premature aging. Our studies demonstrate that maintenance of physiological levels of ceramide phosphoethanolamine by CERT in vivo is required to prevent oxidative damages to cellular components that are critical for viability and normal lifespan of the animal.
Keywords: Animals; Animals, Genetically Modified; Carrier Proteins/*physiology; Ceramides/*metabolism; Drosophila Proteins/deficiency/genetics/*physiology; Drosophila melanogaster/genetics/growth & development/*physiology; Longevity/genetics/*physiology; Oxidative Stress/genetics/*physiology|
Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 104 Issue: 27 Pages: 11364-9 Date: June 27, 2007 PMID: 17592126 |
Rao RP, Yuan C, Allegood JC, Rawat SS, Edwards MB, Wang X, Merrill AH Jr, Acharya U, Acharya JK (2007) Ceramide transfer protein function is essential for normal oxidative stress response and lifespan. Proceedings of the National Academy of Sciences of the United States of America 104: 11364-9.
Comment on This Data Unit