Authors: Dong, Meng-Qiu; Venable, John D; Au, Nora; Xu, Tao; Park, Sung Kyu; Cociorva, Daniel; Johnson, Jeffrey R; Dillin, Andrew; Yates, John R 3rd
Abstract: DAF-2, an insulin receptor-like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.Keywords: Aging/physiology; Animals; Caenorhabditis elegans/genetics/*metabolism/physiology; Caenorhabditis elegans Proteins/genetics/*metabolism; Calcineurin/metabolism; Carbohydrate Metabolism; Gene Expression Regulation; Genes, Helminth; Longevity/physiology; Mass Spectrometry/methods; Models, Animal; Mutation; Proteomics; RNA Interference; Receptor, Insulin/genetics/*metabolism; *Signal Transduction
Date: Aug. 4, 2007
Select reference article to upload
Dong, Meng-Qiu, Venable, John D, Au, Nora, Xu, Tao, Park, Sung Kyu, Cociorva, Daniel, Johnson, Jeffrey R, Dillin, Andrew, Yates, John R 3rd (2007) Quantitative mass spectrometry identifies insulin signaling targets in C. elegans. Science 317: 660-3.