Figure 6: Deletion of AFG3 reduces cytoplasmic translation and extends life span by a Sir2-independent, Gcn4-dependent mechanism

Study: Stress profiling of longevity mutants identifies Afg3 as a mitochondrial determinant of cytoplasmic mRNA translation and aging.
Species: Budding yeast
Assay: RLS
Data: genotype treatment mean median max num pvalue pvalue pvalue
#temperature=30

fob1 - 32.7 35 52 39 - - -
afg3;fob1 - 38.3 40 60 40 2.29E-02 - -

wt - 20.3 19 38 80 - - -
afg3 - 25.5 24 60 180 1.15E-03 - -
sir2;fob1 - 19.7 20 40 39 9.29E-01 1.25E-02 -
sir2;fob1;afg3 - 29.1 30 45 40 1.43E-06 1.42E-02 2.95E-05

sir2;fob1 - 30.8 34 46 20
sir2;fob1;afg3 - 46.0 44 78 20 3.60E-02 - -
sir2;fob1 DR 42.9 50 64 20 1.67E-02 9.14E-01 -
sir2;fob1;afg3 DR 45.3 48 67 20 1.48E-03 8.82E-01 7.56E-01

wt - 21.2 20 38 98 - - -
afg3 - 26.7 26 53 260 2.95E-07 - -
gcn4 - 22.7 21 42 80 2.24E-01 8.08E-04 -
afg3;gcn4 - 18.0 17 50 119 4.10E-03 7.59E-13 4.82E-04

wt - 19.8 19 33 39
afg3 - 24.2 24 45 100 4.77E-03 - -
rpl20b - 33.8 36 60 59 1.41E-08 4.34E-08 -
afg3rpl20b - 30.3 32 52 40 2.23E-05 1.70E-03 1.24E-01
Measurments:
  • fob1_30_32_35_52_39
  • afg3;fob1_30_38_40_60_40
  • wild-type_30_20_19_38_80
  • afg3_30_25_24_60_180
  • sir2;fob1_30_19_20_40_39
  • sir2;fob1;afg3_30_29_30_45_40
  • sir2;fob1_30_30_34_46_20
  • sir2;fob1;afg3_30_46_44_78_20
  • sir2;fob1_30_42_50_64_20
  • sir2;fob1;afg3_30_45_48_67_20
  • wild-type_30_21_20_38_98
  • afg3_30_26_26_53_260
  • gcn4_30_22_21_42_80
  • afg3;gcn4_30_18_17_50_119
  • wild-type_30_19_19_33_39
  • afg3_30_24_24_45_100
  • rpl20b_30_33_36_60_59
  • afg3rpl20b_30_30_32_52_40
  • Comparisons:
  • afg3;fob1 vs. fob1
  • afg3 vs. wild-type
  • sir2;fob1 vs. wild-type
  • sir2;fob1;afg3 vs. wild-type
  • sir2;fob1;afg3 vs. sir2;fob1
  • sir2;fob1 DR vs. sir2;fob1
  • sir2;fob1;afg3 DR vs. sir2;fob1
  • afg3 vs. wild-type
  • gcn4 vs. wild-type
  • afg3;gcn4 vs. wild-type
  • afg3 vs. wild-type
  • rpl20b vs. wild-type
  • afg3rpl20b vs. wild-type

  • ID exp ctr Manipulation intervention mean median max pvalue T Gender epistasis
    90 afg3;fob1_30_38_40_60_40 fob1_30_32_35_52_39 - 17.125382263 14.2857142857 15.3846153846 30°C
    91 afg3_30_25_24_60_180 wild-type_30_20_19_38_80 - AFG3 deletion 25.6157635468 26.3157894737 57.8947368421 30°C
    92 sir2;fob1_30_19_20_40_39 wild-type_30_20_19_38_80 - -2.95566502463 5.26315789474 5.26315789474 30°C
    93 sir2;fob1;afg3_30_29_30_45_40 wild-type_30_20_19_38_80 - 43.3497536946 57.8947368421 18.4210526316 2.95e-05 30°C
    94 sir2;fob1;afg3_30_46_44_78_20 sir2;fob1_30_30_34_46_20 - 49.3506493506 29.4117647059 69.5652173913 30°C
    95 sir2;fob1_30_42_50_64_20 sir2;fob1_30_30_34_46_20 DR 39.2857142857 47.0588235294 39.1304347826 30°C
    96 sir2;fob1;afg3_30_45_48_67_20 sir2;fob1_30_30_34_46_20 DR 47.0779220779 41.1764705882 45.652173913 0.756 30°C
    97 afg3_30_26_26_53_260 wild-type_30_21_20_38_98 - AFG3 deletion 25.9433962264 30.0 39.4736842105 30°C
    98 gcn4_30_22_21_42_80 wild-type_30_21_20_38_98 - 7.07547169811 5.0 10.5263157895 30°C
    99 afg3;gcn4_30_18_17_50_119 wild-type_30_21_20_38_98 - -15.0943396226 -15.0 31.5789473684 0.000482 30°C
    100 afg3_30_24_24_45_100 wild-type_30_19_19_33_39 - AFG3 deletion 22.2222222222 26.3157894737 36.3636363636 30°C
    101 rpl20b_30_33_36_60_59 wild-type_30_19_19_33_39 - 70.7070707071 89.4736842105 81.8181818182 30°C
    102 afg3rpl20b_30_30_32_52_40 wild-type_30_19_19_33_39 - 53.0303030303 68.4210526316 57.5757575758 0.124 30°C
    • 13 comparisons

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